Silencing of SRA1 Regulates ER Expression and Attenuates the Growth of Stromal Cells in Ovarian Endometriosis

Lin, Kejian; Zhan, Hong; Ma, Junyan; Xu, Kang; Wu, Renyi; Zhou, Caiyun; Lin, Jizhen

doi:10.1177/1933719116670036

Cited by 30 publications

(22 citation statements)

References 43 publications

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“…Although EMT is a frequently occurring benign disease, it is characterized by malignant behaviors including recurrence and metastasis ( 68 , 69 ). At present, various pharmacotherapies and other methods have been proposed to treat EMT ( 70 , 71 ). However, their usage has been limited due to strong and obvious adverse effects ( 65 , 72 ).…”

Section: Ccn1/cyr61 and The Female Reproductive Systemmentioning

confidence: 99%

Biological functions and role of CCN1/Cyr61 in embryogenesis and tumorigenesis in the female reproductive system (Review)

Yang

Chen

2017

Mol Med Report

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Cysteine-rich angiogenic inducer 61 (CCN1/Cyr61) is a prompt response transcription product activated by growth factors. As a member of the CCN family, it mediates cell survival, proliferation, differentiation, migration, adhesion and synthesis of the extracellular matrix by binding directly to the integrins and heparin sulfate proteoglycans or activating multiple signaling transduction pathways. It has previously been demonstrated that CCN1/Cyr61 exhibits an important role in the female reproductive system during embryogenesis and tumorigenesis. However, the functions of CCN1/Cyr61 in the female reproductive system have not been systematically investigated, therefore, the primary aim of the present review is to introduce the role and function of CCN1/Cyr61 in the female reproductive system. The current review presents the molecular structure and biological function of CCN1/Cyr61 and provides detailed data on its expression pattern and contribution to the female reproductive system, including the role in embryogenesis and tumorigenesis.

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Section: Ccn1/cyr61 and The Female Reproductive Systemmentioning

confidence: 99%

Biological functions and role of CCN1/Cyr61 in embryogenesis and tumorigenesis in the female reproductive system (Review)

Yang

Chen

2017

Mol Med Report

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“…Wang et al [ 8 ] reported that lncRNA AC002454.1 can participate in the pathogenesis of endometriosis by regulating CDK6 to disrupt the cell cycle. Expression of lncRNA SRA has been found to be associated with expression of estrogen receptor alpha and proliferation of endometriotic stromal cells [ 11 ]. LncRNA H19 expression has recently been shown to decrease in eutopic endometrial tissues, and the restoration of H19 expression in endometriotic stromal cells can promote cell proliferation [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

miR-200c suppresses endometriosis by targeting MALAT1 in vitro and in vivo

et al. 2017

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BackgroundEndometriosis is a common, benign, and estrogen-dependent disease characterized by pelvic pain and infertility. To date, the pathogenesis of endometriosis remains unclear. Recent studies have demonstrated that noncoding RNAs, including microRNAs and long noncoding RNAs, play important roles in the development of endometriosis.MethodsExpression profiling of miRNAs in endometrial tissue was characterized using microarrays. The most differentially expressed miRNAs were confirmed using quantitative reverse transcriptase-polymerase chain reaction analysis in additional ectopic endometrial (n = 27) and normal endometrial (n = 12) tissues. For in-vitro functional studies, 5-ethynyl-2′-deoxyuridine incorporation assay, Transwell assay, and dual-luciferase reporter assay were used to measure the proliferation, migration, and luciferase activity of miR-200c and the predicted targets of miR-200c in primary endometrial stromal cells (HESCs) derived from human endometrial biopsies, respectively. For in-vivo therapeutic interventions, polymeric nanoparticles of polyethylenimine–polyethylene glycol–arginine–glycine–aspartic acid were used for delivery of miR-200c mimic and inhibitor to determine the therapeutic effect of miR-200c in a rat model of endometriosis.ResultsExogenous overexpression of miR-200c inhibited the proliferation and migration of HESCs, which were mainly regulated by metastasis-associated lung adenocarcinoma transcript 1 (MALAT1). In contrast, inhibition of miR-200c promoted the proliferation and migration of HESCs, while the simultaneous silencing of MALAT1 expression exerted the opposite effects. We demonstrated that expression of MALAT1 in ectopic endometrial specimens was negatively correlated with that of miR-200c and that MALAT1 knockdown increased the level of miR-200c in HESCs. Moreover, the transfection of endometrial stromal cells with the miR-200c mimic or MALAT1 siRNAs decreased the protein levels of mesenchymal markers ZEB1, ZEB2, and N-cadherin and increased the protein levels of the epithelial marker E-cadherin. Furthermore, using a rat endometriosis model, we showed that local delivery of the miR-200c mimic significantly inhibited the growth of ectopic endometriotic lesions.ConclusionsThe MALAT1/miR-200c sponge may be a potential therapeutic target for endometriosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0706-z) contains supplementary material, which is available to authorized users.

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“…They are the most common noncoding RNAs and are involved in cell proliferation, differentiation, and apoptosis; all processes central in the pathobiology of endometriosis [21]. Some lnc-RNAs proposed as diagnostic markers of endometriosis include: H19 [22], CHL1-AS2 microRNA and Overcoming the Challenges of Their Use in the Diagnosis of Endometriosis DOI: http://dx.doi.org/10.5772/intechopen.91324 [23,24], AC002454.1 [25], lncRNA SRA (steroid receptor RNA activator) [26], MALAT-1 [27], and LINC01279 [28]. Results of a recent study revealed the lnc-RNA are carried in circulating extracellular vesicles in women with endometriosis [29].…”

Section: Candidate Clinical Markers Of Endometriosismentioning

confidence: 99%

microRNA and Overcoming the Challenges of Their Use in the Diagnosis of Endometriosis

Turpin¹,

Леонова²,

Agarwal³

et al. 2021

Endometriosis

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Endometriosis is a common estrogen dependent and inflammatory disease affecting approximately 176 million women worldwide. Currently, the time between onset of symptoms and a definitive diagnosis has been reported by several international studies to range from 6 to 12 years. Presently, laparoscopic surgery followed by histopathological confirmation of lesions remains the gold standard for diagnosis. In part because of cost and invasiveness, current trends favor reduced laparoscopic surgeries in preference of the non-surgical diagnosis of endometriosis. However, the search for a clinical marker or markers of endometriosis that provide equal or similar sensitivity and specificity to laparoscopy has remained elusive. Thus, the search for a diagnostic test for the diagnosis of endometriosis continues to be a high priority research and clinical issue. Recent studies have reported favorable results with microRNA; however, lack of replication and absence of validation suggest that circulating miRNA may not be reliable for clinical use. Use of different screening platforms together with divergent methods may account for some of the lack or reproducibility in the literature. Herein we critically assess the recent literature and explore sources for discrepant findings. We suggest that prospective studies using validated reference miRNA to normalize results together with improved study design may yet reveal a suitable diagnostic marker or panel of markers for the diagnosis of endometriosis.

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Silencing of SRA1 Regulates ER Expression and Attenuates the Growth of Stromal Cells in Ovarian Endometriosis

Cited by 30 publications

References 43 publications

Biological functions and role of CCN1/Cyr61 in embryogenesis and tumorigenesis in the female reproductive system (Review)

Biological functions and role of CCN1/Cyr61 in embryogenesis and tumorigenesis in the female reproductive system (Review)

miR-200c suppresses endometriosis by targeting MALAT1 in vitro and in vivo

microRNA and Overcoming the Challenges of Their Use in the Diagnosis of Endometriosis

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