Silencing of the Host Factor eIF(iso)4E Gene Confers Plum Pox Virus Resistance in Plum

Wang, Xinhua; Kohalmi, Susanne E.; Svircev, A. M.; Wang, Aiming; Sanfaçon, Hélène; Tian, Lining

doi:10.1371/journal.pone.0050627

Cited by 81 publications

(79 citation statements)

References 71 publications

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“…They are based on either directly targeting the PPV RNA genome through an RNA silencing approach (126) or inactivating host genes that are indispensable for the virus (147). However, such strategies rely on genetically modified plants whose production is, at present, neither fully accepted by the public nor fully effective because only a few accessions of European and Japanese plums are reproducibly transformed (126,139).…”

Section: Breeding Tools and The Selection Of Plum Pox Virus-resistantmentioning

confidence: 99%

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Rimbaud

Dallot

Gottwald

et al. 2015

Annu. Rev. Phytopathol.

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Many plant epidemics that cause major economic losses cannot be controlled with pesticides. Among them, sharka epidemics severely affect prunus trees worldwide. Its causal agent, Plum pox virus (PPV; genus Potyvirus), has been classified as a quarantine pathogen in numerous countries. As a result, various management strategies have been implemented in different regions of the world, depending on the epidemiological context and on the objective (i.e., eradication, suppression, containment, or resilience). These strategies have exploited virus-free planting material, varietal improvement, surveillance and removal of trees in orchards, and statistical models. Variations on these management options lead to contrasted outcomes, from successful eradication to widespread presence of PPV in orchards. Here, we present management strategies in the light of sharka epidemiology to gain insights from this worldwide experience. Although focused on sharka, this review highlights more general levers and promising approaches to optimize disease control in perennial plants.

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Section: Breeding Tools and The Selection Of Plum Pox Virus-resistantmentioning

confidence: 99%

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Rimbaud

Dallot

Gottwald

et al. 2015

Annu. Rev. Phytopathol.

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show abstract

“…In particular, the advent of new technologies such as Targeting-Induced Local Lesions IN Genome (TILLING), EcoTILLING, high-resolution melting (HRM), KeyPoint and next-generation sequencing, may boost the identification of target gene mutants from artificially induced mutant libraries or natural populations, especially in agronomically important crops (Nieto et al, 2007; Hofinger et al, 2009; Rigola et al, 2009; Ibiza et al, 2010; Piron et al, 2010). Genetic resistance can also be generated by silencing or overexpressing the candidate genes depending on the beneficial or the detrimental role of the cellular protein involved in virus long-distance movement (Wang and Krishnaswamy, 2012; Wang et al, 2013). However, these strategies may be of limited use as they may also strongly affect plant macromolecule transport and consequently plant development.…”

Section: Discussionmentioning

confidence: 99%

Viral and Cellular Factors Involved in Phloem Transport of Plant Viruses

Hipper¹,

Brault²,

et al. 2013

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Phloem transport of plant viruses is an essential step in the setting-up of a complete infection of a host plant. After an initial replication step in the first cells, viruses spread from cell-to-cell through mesophyll cells, until they reach the vasculature where they rapidly move to distant sites in order to establish the infection of the whole plant. This last step is referred to as systemic transport, or long-distance movement, and involves virus crossings through several cellular barriers: bundle sheath, vascular parenchyma, and companion cells for virus loading into sieve elements (SE). Viruses are then passively transported within the source-to-sink flow of photoassimilates and are unloaded from SE into sink tissues. However, the molecular mechanisms governing virus long-distance movement are far from being understood. While most viruses seem to move systemically as virus particles, some viruses are transported in SE as viral ribonucleoprotein complexes (RNP). The nature of the cellular and viral factors constituting these RNPs is still poorly known. The topic of this review will mainly focus on the host and viral factors that facilitate or restrict virus long-distance movement.

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“…Recently, eIF4E has been found to provide resistance against Chrysanthemum virus B in chrysanthemum . Also, transgenic plants harbouring a silencing construct of eIF4E and eIF(iso)4E exhibit resistance against Plum pox virus (Wang et al 2013), the virus causing the most economically-devastating viral disease in Prunus species.…”

Section: Roles Of Translation Initiation System In Plantsmentioning

confidence: 99%

Translation initiation in plants: roles and implications beyond protein synthesis

et al. 2015

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Protein synthesis is a ubiquitous and essential process in all organisms, including plants. It is primarily regulated at translation initiation stage which is mediated through a number of translation initiation factors (eIFs). It is now becoming more apparent that in addition to synthesis of proteins, eIFs also regulate various aspects of plant development and their interaction with environment. Translation initiation factors, such as eIF3, eIF4A, eIF4E, eIF4G, and eIF5A affect different processes during vegetative and reproductive growth like embryogenesis, xylogenesis, flowering, sporogenesis, pollen germination, etc. On the contrary, eIF1A, eIF2, eIF4, and eIF5A are associated with interaction of plants with different abiotic stresses, such as high temperature, salinity, oxidative stress, etc. Similarly, eIF4E and eIF4G have roles in interaction with many viruses. Therefore, the translation initiation factors are important candidates for improving plant performance and adaptation. A large number of genes encoding eIFs can functionally be validated and utilized through genetic engineering approaches for better adaptability and performance of plants by inhibiting/minimizing or increasing expression of desired eIF(s).

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Silencing of the Host Factor eIF(iso)4E Gene Confers Plum Pox Virus Resistance in Plum

Cited by 81 publications

References 71 publications

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Viral and Cellular Factors Involved in Phloem Transport of Plant Viruses

Translation initiation in plants: roles and implications beyond protein synthesis

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