Silent Circulation of Ross River Virus in French Polynesia

Aubry, Maïté; Finke, Jérôme; Teissier, Anita; Roche, Claudine; Broult, Julien; Paulous, Sylvie; Desprès, Philippe; Cao-Lormeau, Van-Mai; Musso, Didier

doi:10.1016/j.ijid.2015.06.005

Cited by 49 publications

(57 citation statements)

References 32 publications

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“…Until 2013, DENV was the only arbovirus detected in French Polynesia, causing multiple outbreaks since the 1960s (39,(136)(137)(138). However, a retrospective serosurvey of serum samples collected from 2011 to 2013 supported the existence of silent autochthonous circulation of Ross River virus (RRV) (139).…”

Section: Emergence Of Zikv In the Pacificmentioning

confidence: 99%

Zika Virus

2016

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SUMMARYZika virus (ZIKV) is an arthropod-borne virus (arbovirus) in the genusFlavivirusand the familyFlaviviridae. ZIKV was first isolated from a nonhuman primate in 1947 and from mosquitoes in 1948 in Africa, and ZIKV infections in humans were sporadic for half a century before emerging in the Pacific and the Americas. ZIKV is usually transmitted by the bite of infected mosquitoes. The clinical presentation of Zika fever is nonspecific and can be misdiagnosed as other infectious diseases, especially those due to arboviruses such as dengue and chikungunya. ZIKV infection was associated with only mild illness prior to the large French Polynesian outbreak in 2013 and 2014, when severe neurological complications were reported, and the emergence in Brazil of a dramatic increase in severe congenital malformations (microcephaly) suspected to be associated with ZIKV. Laboratory diagnosis of Zika fever relies on virus isolation or detection of ZIKV-specific RNA. Serological diagnosis is complicated by cross-reactivity among members of theFlavivirusgenus. The adaptation of ZIKV to an urban cycle involving humans and domestic mosquito vectors in tropical areas where dengue is endemic suggests that the incidence of ZIKV infections may be underestimated. There is a high potential for ZIKV emergence in urban centers in the tropics that are infested with competent mosquito vectors such asAedes aegyptiandAedes albopictus.

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Section: Emergence Of Zikv In the Pacificmentioning

confidence: 99%

Zika Virus

2016

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“…The observation that blood samples collected from 2011 to 2013 had only 0.8% seropositivity to Zika virus suggests that the introduction to Polynesia was not long before the identification of the index case (Aubry et al, 2015). The scale of the Polynesian outbreak was unprecedented, with 28000 infections recorded in the first 4 months.…”

Section: Phylogenetics Evolution and Epidemiologymentioning

confidence: 99%

“…This complicates differential diagnosis. Serological testing can, however, distinguish Zika virus infection from that of dengue and chikungunya (Aubry et al, 2015). The virus remained one of the many neglected curiosities of tropical medicine, and no efforts were made to develop a vaccine or treatment in view of its low case numbers and low clinical impact relative to other arboviruses.…”

Section: Introductionmentioning

confidence: 99%

Zika virus: a previously slow pandemic spreads rapidly through the Americas

Gatherer

Kohl

2016

Journal of General Virology

271

208

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“…Fiji, New Caledonia, Samoa and Cook Islands (Kay and Aaskov, 1989). Detection of RRV specific IgG antibodies from blood donors supports the existence of autochthonous RRV transmission and silent circulation in French Polynesia (Aubry et al, 2015).…”

Section: Introductionmentioning

confidence: 73%

Development of a SYBR green I-based quantitative RT-PCR for Ross River virus: Application in vector competence studies and antiviral drug evaluation

Dash

Agarwal

Sharma

et al. 2016

Journal of Virological Methods

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Ross River virus (RRV) is an emerging Alphavirus and is presently endemic in many parts of Oceania. Keeping in mind its emergence, we developed a molecular detection system and utilized it to study vector competence and evaluate activity of antiviral compounds against RRV. A SYBR Green I-based quantitative RT-PCR for detection of RRV was developed targeting the E2 gene, with a detection limit of 100 RNA copies/reaction. The specificity was confirmed with closely related Alphaviruses and Flaviviruses. The assay was applied to study the vector competence of Indian Aedes aegypti for RRV, which revealed 100% infection and dissemination rate with 75% transmission rate. Viral RNA was found in saliva as early as 3day post infection (dpi). Further application of the assay in antiviral drug evaluation revealed the superior in vitro activity of ribavirin compared to chloroquine in Vero cells. Successful demonstration of this assay to detect RRV in low titre mosquito samples makes it a sensitive tool in vector surveillance. This study also showed that Indian Ae. aegypti are well competent to transmit RRV highlighting the risk of its introduction to naïve territories across continents. Further validation of this assay, revealed its utility in screening of potential antivirals against RRV.

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Silent Circulation of Ross River Virus in French Polynesia

Abstract: Our results support the existence of autochthonous RRV transmission and suggest that this pathogen has silently circulated in French Polynesia. These findings raise the question of possible undetected circulation of RRV in other Pacific Island Countries and Territories.

Cited by 49 publications

References 32 publications

Zika Virus

Zika Virus

Zika virus: a previously slow pandemic spreads rapidly through the Americas

Development of a SYBR green I-based quantitative RT-PCR for Ross River virus: Application in vector competence studies and antiviral drug evaluation

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