Silica-alginate-fungi biocomposites for remediation of polluted water

Perullini, Mercedes; Jobbágy, Matı́as; Mouso, Nora; Forchiassin, Flavia; Bilmes, Sara A.

doi:10.1039/c0jm01144d

Cited by 62 publications

(51 citation statements)

References 50 publications

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“…Ca 2+ -ions are easily removed by chelating agents (e.g., phosphates and citrates) and monovalent ions such as Na + and K + , as found in usual biological buffers and media. 22,24,25 Thereby the cross-linking of the Ca-alginate gel is decreased, which results in destabilization of the alginate matrix. This causes osmotic swelling of the gel matrix leading to leakage of the immobilized cells and even to the complete disintegration of the alginate gel network.…”

Section: -12mentioning

confidence: 99%

“…Thus, pure Ca-alginate carriers cannot be maintained for longer periods in aqueous solutions. 24,25 Several strategies have been explored to increase the stability of the alginate matrix, such as the use of divalent cations other than Ca 2+ for cross-linking (e.g., Ba 2+ or Sr 2+ ) 22 or reinforcement by additional cross-linking using polycations, such as poly-Llysine or poly(ethyleneimine).…”

Section: -12mentioning

confidence: 99%

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Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Pannier

Soltmann

et al. 2014

J. Mater. Chem. B

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Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hybrid materials showed improved stability in salt-containing solutions compared to alginate gels gelled by traditional methods using Ca 2+ -ions. Cell arrays were patterned by printing nanolitre-scale drops of sodium alginate/cell suspension using a noncontact micro-dosage system which allows the printing of solutions of high viscosity. Cultures of the green microalga Chlorella vulgaris were immobilized within the newly developed alginate/silica hydrogels in order to demonstrate the potential of the hybrid matrix for the design of cell-based detection systems. The herbicide atrazine as well as copper ions have been used as model toxicants.Short-term toxicity tests (exposure time: 1 h) have been carried out using atrazine and changes in chlorophyll a (Chl a) fluorescence were measured by imaging pulse amplitude modulated-fluorometry (Imaging-PAM). C. vulgaris cells immobilized within alginate/silica hydrogels demonstrated a highly reproducible response pattern and compared well to freely suspended cells. Activity and response sensitivity of immobilized cells to atrazine was largely maintained for up to 8 weeks, especially when stored under cool conditions in the dark. Furthermore, immobilized cells could be repeatingly used for short-term toxicity tests as atrazine produces a reversible inhibition of photosynthesis.

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Section: -12mentioning

confidence: 99%

Section: -12mentioning

confidence: 99%

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Pannier

Soltmann

et al. 2014

J. Mater. Chem. B

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“…Before the immobilization procedure, the filtered mycelium was processed using a commercial homogenizer (Taurus, Portugal). Figure 1 shows the encapsulation procedure used for fungi immobilization, which was adapted from Perullini et al (2010) and Duarte et al (2012). First, P. sajor caju mycelium was encapsulated in calcium alginate by dropping a suspension of mycelium containing 0.50 % of fungus (dry weight) and 1.25 % (w/v) sodium alginate into a 0.10 M CaCl 2 aqueous solution (needle diameter of 1.2 mm).…”

Section: Biological Materials and Culture Conditionsmentioning

confidence: 99%

“…In the use of aerobic treatments regarding fungi, there is still some constrains arising from the microorganismenvironment interactions such as: (1) growth imbalance caused the introduction of foreign organisms that can be more resilient than the natives; (2) microorganisms metabolism hindered by the presence of large amounts of pollutants (Perullini et al 2010). These drawbacks can be overcome by the use of immobilized microorganisms in a matrix such as silica since it allows: (1) the protection of cells or microorganisms from harmful environments; (2) the control of their surrounding environment and of their concentration (Kato et al 2005;Meunier et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Removal of phenolic compounds in olive mill wastewater by silica–alginate–fungi biocomposites

Duarte

Justino

Panteleitchouk

et al. 2013

Int. J. Environ. Sci. Technol.

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This study aims to attempt a treatment strategy based on fungi immobilized on silica-alginate (biocomposites) for removal of phenolic compounds in olive oil mill wastewater (OMW), OMW supplemented (OMWS) with phenolic compounds and water supplemented (WS) with phenolic compounds, thus decreasing its potential impact in the receiving waters. Active (alive) or inactive (death by sterilization) Pleurotus sajor caju was encapsulated in alginate beads. Five beads containing active and inactive fungus were placed in a mold and filled with silica hydrogel (biocomposites). The biocomposites were added to batch reactors containing the OMW, OMWS and WS. The treatment of OMW, OMWS and WS by active and inactive biocomposites was performed throughout 28 days at 25°C. The efficiency of treatment was evaluated by measuring the removal of targeted organic compounds, chemical oxygen demand (COD) and relative absorbance ratio along the time. Active P. sajor caju biocomposites were able to remove 64.6-88.4 % of phenolic compounds from OMW and OMWS and 91.8-97.5 % in water. Furthermore, in the case of OMW there was also a removal of 30.0-38.1 % of fatty acids, 68.7 % of the sterol and 35 % of COD. The silica-alginate-fungi biocomposites showed a high removal of phenolic compounds from OMW and water. Furthermore, in the application of biocomposites to the treatment of OMW it was observed also a decrease on the concentration of fatty acids and sterols as well as a reduction on the COD.

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“…5,6 Additionally, with the emerging advances in nanotechnology, innovative approaches such as the integration of nanomaterials and microorganisms provide access to novel functional materials. Some of these studies focus on interfacing nanomaterials with biological cells to detect biocomponents or to investigate biological phenomena, 7 in particular bioremediation, [8][9][10] the synthesis of bio-templated materials for various novel applications such as supercapacitors, 11 surface enhanced Raman scattering (SERS) substrates, 12 and lithium storage, 13 or for immobilization purposes.…”

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confidence: 99%

Microencapsulation of bacterial strains in graphene oxide nano-sheets using vortex fluidics

et al. 2015

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Wrapping bacterial cells with graphene oxide sheets using a vortex fluidic device (VFD) effectively limits cellular growth for a certain time period whilst sustaining biological activity. This simple and benign method in preparing such a composite material relies on the shear within the film in the device without compromising the cellular viability. In principle, the process is scalable for large volumes, for operating the VFD(s) under continuous flow mode. Moreover, acquiring SEM images was possible without precoating the composite material with a metallic film, with limited charging effects. This establishes the potential for interfacing material with graphene oxide, which could be extended to more conductive graphene layers, as an effective approach for simplifying characterization using SEM.

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Silica-alginate-fungi biocomposites for remediation of polluted water

Cited by 62 publications

References 50 publications

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Removal of phenolic compounds in olive mill wastewater by silica–alginate–fungi biocomposites

Microencapsulation of bacterial strains in graphene oxide nano-sheets using vortex fluidics

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