Silica-based colloid centrifugation enhances sperm quality in cockerel semen

Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol

“…In the present study, the working solution used for SP centrifugation was actually the glycerol removal solution from our previous study ( Lin et al, 2020 , 2023 a). A mixture of 36% Percoll and 80 mM sucrose was prepared in phosphate buffered saline (PBS), and 2 mL of the SP mixture was transferred into a 15 mL centrifuge tube.…”