Silica‐immobilized enzymes for multi‐step synthesis in microfluidic devices

Luckarift, Heather R.; Ku, Bosung; Dordick, Jonathan S.; Spain, Jim C.

doi:10.1002/bit.21447

Cited by 71 publications

(48 citation statements)

References 40 publications

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“…Further investigations using R5 for biosilica formation have led to immobilization of a variety of enzymes for sensor capabilities, catalytic applications, and novel biomaterial production. [29][30][31][32][33][34][35][36][37][38][39] In a few cases, the applicable enzymes themselves catalyze biomineralization reactions, which result in their self-immobilization and retention of activity within the inorganic matrix without addition of a separate biomineralizing peptide. 40,41 Whether part of a physiological function or of man-made design, many peptides that catalyze biomineralization reactions (e.g., histidine-rich proteins, [42][43][44] noble metal nanoparticle-forming peptides, 45,46 silaffins, silicatiens, and other lysine-rich proteins 47 ) have an overall positive charge and are amphiphilic in nature; characteristics that are also shared with many AMPs.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Bioinorganic Antimicrobial Peptide Nanoparticles with Potential Therapeutic Properties

2008

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Amphiphilicity and cationicity are properties shared between antimicrobial peptides and proteins that catalyze biomineralization reactions. Merging these two functionalities, we demonstrate a reaction where a cationic antimicrobial peptide catalyzes self-biomineralization within inorganic matrices. The resultant antimicrobial peptide nanoparticles retain biocidal activity, protect the peptide from proteolytic degradation, and facilitate a continuous release of the antibiotic over time. Taken together, these properties demonstrate the therapeutic potential of selfsynthesizing biomaterials that retain the biocidal properties of antimicrobial peptides.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Bioinorganic Antimicrobial Peptide Nanoparticles with Potential Therapeutic Properties

2008

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“…Microfabricated flow reactor systems with immobilized enzymes are of great recent importance in the biopharmaceutical industry, namely, in the production of specific antibiotics, highly toxic antitumor drugs [41,42], or drug delivery agents [43]. The main applications of continuous-flow biochemical microreactor systems utilizing immobilized enzymes for bioconversion are summarized in Table 1.…”

Section: Continuous-flow Biochemical Microreactors For Biocatalysis Amentioning

confidence: 99%

Continuous-Flow Biochemical Reactors: Biocatalysis, Bioconversion, and Bioanalytical Applications Utilizing Immobilized Microfluidic Enzyme Reactors

Hajba

Guttman

2016

J Flow Chem

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The utilization of continuous-flow biochemical reactors, including biocatalysis, biotransformation, and biochemical interaction based flow-analytical systems, and enzyme reactors are recently the focus of attention to produce fine biochemicals and also show great potential in bioanalytical applications. Continuous-flow biochemical processes implemented in microstructured reactors enable short development time to production scale utilizing enzymatic processes to efficiently fulfill the current needs of the fine biochemical and pharmaceutical industry. Immobilization of the enzymes is preferable because it usually enhances their stability, and in some instances, immobilized enzymes can even be reused multiple times. In this review on the continuous-flow biochemical reactors, first the enzyme immobilization strategies will be briefly discussed followed by summarizing the recent developments in the field of immobilized enzyme microflow reactors for biocatalysis, bioconversion and bioanalytical purposes.

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“…The application of enzyme-immobilized microreactor for multistep synthesis has also been demonstrated (Luckarift et al, 2007). Three separated microfluidic devices, which possess metallic zinc, silica-immobilized hydroxyaminobenzene mutase, and silica-immobilized peroxidase within a microchannel, were prepared and connected sequentially.…”

Section: Enzyme-immobilized Microreactor For Processingmentioning

confidence: 99%

Enzymatic Processing in Microfluidic Reactors

Miyazaki

Honda

Yamaguchi

et al. 2008

Biotechnology and Genetic Engineering Reviews

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Microreaction technology is an interdisciplinary area of science and engineering. It has attracted the attention of researchers from different fields in the past few years and consequently, several microreactors have been developed. Enzymes are organic catalysts used for the production useful substances in an environmentally friendly way, and have high potential for analytical applications. However, relatively few enzymatic processes have been commercialized because of problems in the stability of enzyme molecule, and the cost and efficiency of the reactions. Thus, there have been demands for innovation in process engineering particularly for enzymatic reactions, and microreaction devices can serve as efficient tools for the development of enzyme processes. In this review, we summarize the recent advances of microchannel reaction technologies and focus our discussion on enzyme microreactors. We discuss the manufacturing process of microreaction devices and the advantages of microreactors compared with the conventional reactors. Fundamental techniques for enzyme microreactors and important applications of this multidisciplinary technology in chemical processing are also included in our topics. Biotechnology and Genetic Engineering Reviews -Vol. 25, 405-428 (2008) *To whom correspondence may be addressed (m.miyazaki@aist.go.jp or maeda-h@aist.go.jp) #Present address: Department of Pharmacology, Yamaguchi University Graduate School of Medicine, Yamaguchi 755-8505, Japan Abbreviations: NTA, N,N-bis(carboxymethyl)glycine, which binds Nickel (II) ion, and usually applied for identification/purification of histidine tag of the engineered enzyme molecule; LIGA, Lithography, Electroplating, and Molding; PEEK, poly(ether ether ketone).

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Silica‐immobilized enzymes for multi‐step synthesis in microfluidic devices

Cited by 71 publications

References 40 publications

Synthesis of Bioinorganic Antimicrobial Peptide Nanoparticles with Potential Therapeutic Properties

Synthesis of Bioinorganic Antimicrobial Peptide Nanoparticles with Potential Therapeutic Properties

Continuous-Flow Biochemical Reactors: Biocatalysis, Bioconversion, and Bioanalytical Applications Utilizing Immobilized Microfluidic Enzyme Reactors

Enzymatic Processing in Microfluidic Reactors

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