Silica-Iron Oxide Magnetic Nanoparticles Modified for Gene Delivery: A Search for Optimum and Quantitative Criteria

Abstract

Surface decoration of the silica-iron oxide nanoparticles with a PEI-to-iron w/w ratio of 10-12% yields stable aqueous suspensions, allows for efficient viral gene delivery and labeled cell detection by MRI.

Human cellular CYBA UTR sequences increase mRNA translation without affecting the half-life of recombinant RNA transcripts

“…Magnetofection was performed by using SoMag5 magnetic nanoparticles. Further information related to the composition and characterization of these magnetic nanoparticles can be found elsewhere49. These particles were used at a ratio of 1 μg mRNA per 0.5 μg Fe.…”

Manipulation of Schwann cell migration across the astrocyte boundary by polysialyltransferase-loaded superparamagnetic nanoparticles under magnetic field

“…39,40 The surface of SPIONs was further modified through the attachment of a cationic polymer, such as PEI, rendering it positively charged and thus apt for cellular internalization. [41][42][43] PEI-SPIONs could effectively absorb negatively charged PST plasmids via electrostatic attraction. 44,45 After magnetofection, the PEI-SPIONs/PST complex was readily internalized into cells by magnetic force, as confirmed by TEM, which revealed the presence of SPIONs within cells.…”

Nanomagnetic Activation as a Way to Control the Efficacy of Nucleic Acid Delivery

“…These particles associate efficiently with such nucleic acids as pDNA, siRNA, and mRNA in duplexes and triplexes with different enhancers and different viral vectors used for efficient delivery into cells in vitro, ex vivo, and in vivo (16,22). For non-specific cell labeling, we used SO-Mag5 iron oxide nanoparticles (17) and antibody-modified magnetic microbeads for specific cell labeling. Transfections were performed with non- Fig.…”

“…Core/shell-type iron oxide magnetic nanoparticles (MNPs) were synthesized by precipitating Fe(II)/Fe(III) hydroxide from an aqueous salt solution, followed by transformation into magnetite in an oxygen-free atmosphere, with immediate spontaneous adsorption of the shell components, as described elsewhere (16,17). For formulation of the magnetic lipoplexes, the particles with a surface coating of 25-kDA branched polyethylenimine (PEI-25 Br ) combined with the fluorinated surfactant ZONYL FSA (lithium 3-[2-(perfluoroalkyl) ethylthio] propionate) were used, hereafter referred to as PEI-Mag2 (18).…”

“…For formulation of the magnetic lipoplexes, the particles with a surface coating of 25-kDA branched polyethylenimine (PEI-25 Br ) combined with the fluorinated surfactant ZONYL FSA (lithium 3-[2-(perfluoroalkyl) ethylthio] propionate) were used, hereafter referred to as PEI-Mag2 (18). For non-specific magnetic cell labeling, the MNPs with a coating resulting from the condensation of tetraethyl orthosilicate (TEOS) and 3-(trihydroxysilyl) propylmethylphosphonate (THPMP), yielding a silicon oxide layer with surface phosphonate groups, were used and will hereafter be referred to as SO-Mag5 (17). The aqueous MNP suspensions were sterilized using 60 Co gamma-irradiation at a dose of 25 kGy.…”