Silica microspheres functionalized with the iminodiacetic acid/copper(II) complex as a peroxidase mimic for use in metal affinity chromatography-based colorimetric determination of histidine-tagged proteins

Gökçal, Burcu; Kip, Çiğdem; Şahinbaş, Dilek; Çelik, Eda; Tuncel, Alï

doi:10.1007/s00604-019-4087-0

Cited by 22 publications

(11 citation statements)

References 36 publications

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“…Today, inorganic materials such as silica, carbon-based materials, magnetic nanoparticles, and metal-organic frameworks (MOFs) are widely used as support materials in the synthesis of IMAC sorbents. Several silica-based sorbents both in the magnetic and non-magnetic forms were successfully tried for the enrichment of phosphorylated proteins [44,47,54,[60][61][62]. Silica-based supports have several advantages such as good mechanical stability, rigid 3D structure, tunable pore size, large surface area, and facile derivatization [63].…”

Section: Techniques Advantages Disadvantagesmentioning

confidence: 99%

“…The schematic illustration of His-tagged protein purification by using Cu(II) immobilized-SiO 2 based magnetic sorbent [60] samples [62]. The equilibrium adsorption capacity of the synthesized IMAC sorbent was calculated as 1046.5 mg/g for β-casein and 93.5 mg/g for BSA [62].…”

Section: F I G U R Ementioning

confidence: 99%

“…GFP was isolated with isolation yields up to 68% and high purity (>95%) [65]. Magnetic silica microspheres functionalized with the iminodiacetic acid/copper(II) complex were also synthesized and their selectivity for His‐tagged proteins was evaluated [60]. The purification of GFP from E. coli extract was performed by the developed sorbent.…”

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

“…The purification of GFP from E. coli extract was performed by the developed sorbent. On the other hand, the same sorbent was also utilized as a nanozyme with peroxidase‐like activity, working based on IMAC interaction for colorimetric determination of His‐tagged proteins [60]. The schematic illustration of the affinity capture of GFP as a typical His‐tagged protein by the immobilized Cu(II) ions on a silica (SiO 2 ) based magnetic sorbent is shown in Figure 3 [60].…”

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

“…In the last decade, there has been an increasing trend in the enrichment of biomarkers with IMAC in various biotechnological and biomedical applications before mass spectrometric analysis for their detection in biological samples [7, 9, 42–44, 47–50, 14,15,51–60]. IMAC has become an important technique in the purification of histidine‐tagged proteins and phosphoproteins in recent years.…”

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

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Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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Section: Techniques Advantages Disadvantagesmentioning

confidence: 99%

Section: F I G U R Ementioning

confidence: 99%

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

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Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Protein‐based metal bio‐cleaner for detoxification of wastewater

Sisila

Janeena

Suresh

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND Copper is an essential redox‐active transition metal accessible in the very least amount in healthy living cells. Exposure to heavy metals through contaminated soil, the food chain and drinking of contaminated groundwater affects cellular metabolism in the human body. Hence, developing innovative next‐generation approaches with a dual function such as efficient detection (sensor) and removal of heavy metals (Cu2+) in the environment is of paramount importance. Our study deals with an engineered biologically compatible green fluorescent protein (GFP) with metal‐binding amino acid (3‐aminotyrosine) for effective sensing and removal. RESULTS The fluorescent‐based sensing of congener protein (amGFP) with heavy metal Cu2+ was extensively explored through fluorescence, circular dichroism spectroscopic and computational simulation approaches. The amGFP exhibits a low dissociation constant value of 5.25 μmol L−1. In addition, the engineered congener protein was used as a bio‐cleaner to remove Cu2+ from environmental samples. The copper binding capacity of engineered protein (amGFP, 346.46 μg mL−1) showed a 1.6‐fold increase compared with native protein (GFP, 214 μg mL−1). GFP and amGFP immobilized on ethylenediamine‐functionalized granular activated carbon (FGAC) were applied to environmental samples; FGAC‐immobilized amGFP exhibited Cu2+ removal of about 85%, which was twofold higher than that of FGAC‐immobilized GFP. CONCLUSIONS The results prove that amGFP was able to bind more copper than native GFP. This is an early attempt to develop a genetically encoded fluorescent protein that could be used to effectively remove Cu2+ from wastewater. © 2022 Society of Chemical Industry (SCI).

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