Silica Nanoparticles Encapsulated Cichorium Pumilum as a Promising Photosensitizer for Osteosarcoma Photodynamic Therapy: In-vitro study

Makhadmeh, Ghaseb N.; Abuelsamen, Abdulsalam; Al-Akhras, M-Ali H.; Aziz, Azlan Abdul

doi:10.1016/j.pdpdt.2022.102801

Cited by 9 publications

(7 citation statements)

References 29 publications

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“…The process commenced by blending 200 mL of pure water, a minuscule quantity of ammonia (0.1 mL), and 5.5 g of Tween 80, which were constantly mixed for roughly 15 min. Next, the pH level was gauged and regulated to 9.0, and subsequently, 1-butanol was added, and its mixture was again stirred for an extra 5 min [35]. The solution was then transferred to a reactor enclosed by aluminum foil, and 15 mL of SF was blended in and mixed at 320 rpm and 27 • C for one hour.…”

Section: Methodsmentioning

confidence: 99%

“…The culture was then left overnight to allow cell growth. They were then washed three times with PBS and incubated with 5 mL of fresh DMEM medium containing SF-encapsulated SiNPs in all concentrations [35]. All the samples were exposed to the light source (intensity ~110 mW/cm 2 red laser, with a 40 cm distance between the sample and the laser source) for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…After undergoing PDT, the ratio of living cells in the second sample was calculated as a percentage compared to the untreated sample to determine cell viability [35]. The cytotoxicity of MCF-7 cells with encapsulated SF-SiNPs was assessed by comparing the number of untreated control cells to those treated with SF-SiNPs and incubated for 7 h without exposure to light in a controlled environment.…”

Section: Methodsmentioning

confidence: 99%

“…As a result, the concentration efficacy was calculated to be 50% using the efficacy equation. Nevertheless, the cell death percentage rose as the concentration of encapsulated and naked SF increased during light exposure due to the production of singlet oxygen or ROS resulting from SF's exposure to light [35]. Furthermore, the cytotoxicity generated in the absence of light was elevated as the concentration of SF increased.…”

Section: The Optimal Concentration Of Encapsulated and Naked Sfmentioning

confidence: 99%

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Advancing Photodynamic Therapy Efficiency on MCF-7 Breast Cancer Cells through Silica Nanoparticles-Safranin Encapsulation: In-Vitro Evaluation

et al. 2023

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Efficient drug delivery to target tissue is a major challenge in many cancer treatment modalities. Silica nanoparticles (SiNPs) have been identified as an ideal drug carrier due to their unique properties. In Photodynamic therapy (PDT), one of the key challenges in utilizing photosensitizers (PS) lies in effectively delivering the PS to the targeted tissue. Using Silica nanoparticles encapsulation will effectively prevent the leakage of entrapped PS from the particles, protects against reduction by the retinal endothelial system, and reduces PS toxicity. In this study, Silica nanoparticles (SiNPs) were used as carriers for Safranin (SF) as a photosensitizer agent to treat MCF-7 breast cancer cells in vitro. The SiNPs nanoparticles were synthesized, and their size and shape were measured using Transmission Electron Microscopy (TEM). Cytotoxicity was evaluated for different concentrations of encapsulated and naked SF. The optimal concentrations and exposure times required to eliminate the MCF-7 under light (Intensity ~110 mW/cm2, red laser) were determined. The results indicated that encapsulated SF by SiNPs exhibited higher efficacy than naked SF with a +50% concentration efficacy and +78% exposure time efficacy. This confirmed the superior ability of encapsulated SF to eliminate MCF-7 cells compared to naked SF. The use of synthesized silica nanoparticles loaded with SF improved photodynamic therapy by increasing the bioavailability of SF in the target cells. Our results demonstrate that SiNP encapsulation significantly improves the efficacy of SF in eliminating MCF-7 cells compared to bare SF. This study underscores the potential of SiNPs as a drug delivery system for photodynamic therapy and could pave the way for developing more effective cancer treatments.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: The Optimal Concentration Of Encapsulated and Naked Sfmentioning

confidence: 99%

See 2 more Smart Citations

Advancing Photodynamic Therapy Efficiency on MCF-7 Breast Cancer Cells through Silica Nanoparticles-Safranin Encapsulation: In-Vitro Evaluation

et al. 2023

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“…In this context, superparamagnetic iron oxide nanoparticles (SPIONs) have attracted significant attention from researchers for their exceptional characteristics, such as high relaxivity, superior magnetic properties, non-toxicity, and the ability to target specific cells or tissues. Compared to Gd-based contrast agents, SPIONs have proved to be promising nanomaterials for a wide range of biomedical applications [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

A Novel One-Pot Synthesis of PVP-Coated Iron Oxide Nanoparticles as Biocompatible Contrast Agents for Enhanced T2-Weighted MRI

et al. 2023

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A contrast agent with specific characteristics is essential for high-quality magnetic resonance imaging (MRI). It plays a crucial role in enhancing the visibility of certain tissues and structures, making it imperative for diagnostic procedures. Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as a promising alternative to traditional contrast agents for MRI due to their non-toxicity and superior magnetic properties. However, a suitable surface coating strategy is needed to produce polymer-coated SPIONs with controllable sizes in order to enhance their stability and biocompatibility. This study presents a novel one-pot synthesis method for the production of highly stable polyvinylpyrrolidone (PVP)-coated SPIONs. By systematically manipulating the physicochemical properties of SPIONs, the effect of different molecular weights of PVP was studied. The results showed that SPIONs coated with PVP with molecular wight 40,000 g/mol (40 K) exhibited a high magnetization (Ms = 48.4 emu/g), an average size distribution (11.61 nm), and excellent stability. The relaxivity of coated and uncoated SPIONs was investigated using MRI images. The results revealed that the (r2/r1) ratio of PVP40K-SPIONs was 72.55, compared to 55.72 for the bare SPIONs, making them a highly promising T2-contrast agent for future development of MRI applications. This study opens new avenues for the development of biocompatible and stable SPIONs for improved medical diagnostic and imaging.

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Phytochemistry, pharmacological applications, and therapeutic effects of green synthesized nanomaterials using Cichorium species—a comprehensive review

Sishu,

Selvaraj

2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Silica Nanoparticles Encapsulated Cichorium Pumilum as a Promising Photosensitizer for Osteosarcoma Photodynamic Therapy: In-vitro study

Cited by 9 publications

References 29 publications

Advancing Photodynamic Therapy Efficiency on MCF-7 Breast Cancer Cells through Silica Nanoparticles-Safranin Encapsulation: In-Vitro Evaluation

Advancing Photodynamic Therapy Efficiency on MCF-7 Breast Cancer Cells through Silica Nanoparticles-Safranin Encapsulation: In-Vitro Evaluation

A Novel One-Pot Synthesis of PVP-Coated Iron Oxide Nanoparticles as Biocompatible Contrast Agents for Enhanced T2-Weighted MRI

Phytochemistry, pharmacological applications, and therapeutic effects of green synthesized nanomaterials using Cichorium species—a comprehensive review

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