Silicate entrapped columns — new columns designed for capillary electrochromatography

Chirica, Gabriela S.; Remcho, Vincent T.

doi:10.1002/(sici)1522-2683(19990101)20:1<50::aid-elps50>3.0.co;2-o

Cited by 145 publications

(73 citation statements)

References 18 publications

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“…This phase showed high chiral recognition ability for phenylalanine, but was weak for tyrosine and phenylglycine. Chirica and Remcho [542] prepared a monolithic column by flushing a capillary packed with a polymer, containing l-Dns-phenylalanine as an imprint molecule, with an aqueous potassium silicate solution followed by heating from 40 to 1608C for several days.…”

Section: Monolithic Cspsmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

225

135

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This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.

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Section: Monolithic Cspsmentioning

confidence: 99%

Chiral separation by chromatographic and electromigration techniques. A Review

Gübitz

Schmid

2001

Biopharm & Drug Disp

225

135

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“…MIPs have shown promising features as novel stationary phases in liquid chromatography (LC) [11] and recently also in CEC [12,13]. MIP based stationary phases in CEC have been prepared in situ as superporous monoliths [14][15][16], particles entrapped in different matrixes [17,18] and as wall coatings in open tubular capillaries [19,20].…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted microparticles for capillary electrochromatography: Studies on microparticle synthesis and electrolyte composition

2001

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The use of molecularly imprinted polymer (MIP) microparticles in a partial filling application of capillary electrochromatography (CEC) has previously been shown successful for the enantiomer separation of propranolol. In this investigation, the influence of some important parameters in the preparation protocol, i.e., template to monomer ratio, type of cross-linker and functional monomers, and the effect of separation condition, i.e., organic modifier content, pH and the temperature of the column, on the electrochromatographic behavior of the MIP microparticles were studied. It was found that ethyleneglycol dimethacrylate (EDMA), having two reactive double bonds, was superior in terms CEC performance to trimethylpropane trimethacrylate (TRIM) and pentaerythritol tetraacrylate (PETEA) having three and four double bonds, respectively. The use of weak functional monomers, i.e., monomers lacking a strong interaction with the template, was shown to increase the separation efficiency. It was found that the template to functional monomer ratio had a pronounced influence on the MIP microparticle partial filling CEC performance as well as the size of the obtained microparticles. The use of a partial filling technique realizes the use of a new MIP phase in every new separation as well as the ability of altering the selectivity of the separation column and length of the MIP without the need for column switching.

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“…We produced 20.6 grams of wet biomass from three 24 L cultures, whereas De Jong et al (1976) To compare the rate of dissolution of biogenic calcite to that of reagent grade calcite we opted to use calcium carbonate. We vacuum packed our column using a modified technique described by Lynen et al (2005) and Chirica et al (1999). Simply, the column is opened at both ends and a membrane is placed at one end of the column and sealed with a capillary fitting.…”

Section: Discussionmentioning

confidence: 99%