Silicon and agar on in vitro development of cockscomb (Amaranthaceae)

Abstract

The objective of this work was to evaluate the effect of silicon and agar concentrations on cockscomb (Celosia cristata) (Amaranthaceae) development in vitro. Phytotechnical, anatomical, and ultrastructural analyses were carried out in a completely randomized design, in a 4x3 factorial arrangment, with four silicic acid concentrations (0.0, 0.5, 1.0, and 1.5 g L-1) and three agar concentrations (5.5, 8.0, and 10.5 g L-1), totaling 12 treatments with seven replicates. Celosia cristata explants were inoculated in vitro in a Murashige & Skoog (MS) culture medium, with 30 g L-1 sucrose. The analyses were conducted 30 days after inoculation. Phytotechnical traits improve with agar concentration of 8.0 g L-1, and the association of this concentration with 1.0 g L-1 silicon also contributes to the improvement of anatomical attributes, especially of leaf blade width and thickness of the palisade and spongy parenchyma, which contributes to the development of C. cristata. None of the evaluated combinations of agar and silicon contributes to Si deposition on the foliar epidermis of this ornamental species.

Silicon in the Anatomy and Physiology of Banana Plant Leaves Under Temporary Immersion Bioreactors

“…(Asmar et al, 2013), anthurium (Anthurium adreaenum cv. Rubi) (Dias et al, 2014),cape gooseberry (Physalis peruviana L.) (Rezende et al, 2018), cockscomb (Celosia cristata) (Assis et al, 2018) and bromeliad (Aechmea blanchetiana) (Martins et al, 2019).…”

Impact of saline solution on growth and photosystem II during in vitro cultivation of Bromelia antiacantha (Bromeliaceae)

“…This aspect needs to be observed in micropropagation programs, since plants need to absorb nutrients from the culture medium until they can be transplanted to ex vitro conditions (Akin et al 2017;Poothong et al 2017). The benefits of optimizing the nutrients in culture media are well documented for a wide range of species and applications (Hand & Reed 2014;Tavares et al 2015Tavares et al , 2017Wada et al 2015;Leljak-Levanic et al 2016;Akin et al 2017;Dias et al 2017;Poothong et al 2017;Silva et al 2017b;Assis et al 2018;Pérez-Alonso et al 2018). The success of micropropagation can depend on the nature and concentration of mineral nutrients in the medium, and this can vary and have different effects depending on the species, genotype or technique employed (Akin et al 2017;Poothong et al 2017).…”