Silicon Fertilizer and Microbial Agents Changed the Bacterial Community in the Consecutive Replant Soil of Lilies

Abstract

Crop replanting leads to soil degradation and soil productivity reduction, which is a challenge for sustainable agricultural development. We previously found that silicon fertilizers combined with additional microbial agents are an effective means to alleviate problems that occur in a variety of Chinese lily during replanting, but little is known about the changes in microbial structure during this process. In the present study, we applied four treatments: CK (control), SF (silicon fertilizer), MF (microbial agents), and SMF (combination of silicon fertilizer and microbial agents). We treated the soil constantly for three years and investigated the bacterial community structure and some specific microbial groups in the soil of the lily root zone using 16S rRNA high-throughput sequencing analysis. The results showed that silicon fertilizer and microbial agent treatment significantly improved the growth status of the plants and changed the diversity and structure of the bacterial community in the soil. The genus Pseudomonas significantly increased in the SF treatment, and the phylum Actinobacteria and the genera Nordella, Devosia, and Rhodoplanes significantly increased in the SMF treatment. The genera Nordella, Pedomicrobium, and Chthoniobacter correlated with the seedling index or available silicon content. In addition, the two genera Gaiella and Nocardioides were the key species linking the bacterial community in the soil. The soil physicochemical properties played an important role in restoring the soil bacterial community structures. In conclusion, silicon fertilizer and microbial agents changed the diversity and structure of the bacterial community. Under the fertilizer supplement model, the enrichment of the phylum Actinobacteria and the genus Pseudomonas played an important role in improving soil health and alleviating CRPs in lilies. In addition, organic matter, available phosphorus, available potassium, and available silicon were found to be the most important factors that have a great impact on the restoration of bacterial community structures.

Two Bacillus spp. Strains Improve the Structure and Diversity of the Rhizosphere Soil Microbial Community of Lilium brownii var. viridulum

“…These findings are consistent with a previous study that integrated biological control methods and effectively suppressed tobacco bacterial wilt by regulating the soil physicochemical properties, and promoting beneficial and antagonistic bacteria in the rhizosphere soil [52]. The longer arrows in the RDA ordinations (Figure 1) indicated that pH had a stronger effect on bacteria and fungi than the other physicochemical properties, which is consistent with the findings of Shi et al [53] and Yu et al [54]. A redundancy analysis was performed to examine the relationship between the soil edaphic properties and the diversity of microbial communities in the four groups of soil samples.…”

Effects of grazing in different intensities and 10‐year grazing exclusion on soil bacterial community composition and interaction complexity in alpine grasslands of the Qinghai‐Tibet Plateau

“…Actinobacteria has important functions in improving soil conditions through debris decomposition, and in promoting microbial growth via secondary metabolites and enzyme production (Selim et al, 2021). While the Actinobacteria was less abundant with higher grazing intensity (Ling et al, 2022; Yu et al, 2022). These findings suggested that the extreme habitat induced by heavy grazing severely influenced the soil bacterial structure and the associated functions.…”

“…Bacteroidota and Firmicutes which are intestinal microorganisms, were also abundant in grazing areas, possibly due to fecal excretion (Fan & Pedersen, 2021). However, Proteobacteria is known for the ability to decompose recalcitrant carbon, but was unexpectedly to decrease with increasing grazing intensity (He et al, 2022;Yu et al, 2022). Actinobacteria has important functions in improving soil conditions through debris decomposition, and in promoting microbial growth via secondary metabolites and enzyme production (Selim et al, 2021).…”

Divergent taxonomic responses of below-ground microbial communities to silicate fertilizer and biofertilizer amendments in two rice ecotypes

“…We observed that bacterial phyla, including Proteobacteria, Acidobacteria, Actinobacteria, and two fungal phyla Ascomycota and Basidiomycota are the most prevalent groups that were consistently enriched in that perspective order, irrespective of the cultivar type and treatment (Figure 1). A recent study on the bacterial communities has shown the dominant trends of these phyla, that were affected under longterm fertilization and both silicate and microbial agent applications (Li et al, 2021;Yu et al, 2022). Both Ascomycota and Basidiomycota, are the two most dominant fungal phyla that participate in degradation processes (decomposers) (Morrison et al, 2016;Egidi et al, 2019).…”