Silicon–hydroxyapatite bioactive coatings (Si–HA) from diatomaceous earth and silica. Study of adhesion and proliferation of osteoblast-like cells

Abstract

The aim of this study consisted on investigating the influence of silicon substituted hydroxyapatite (Si-HA) coatings over the human osteoblast-like cell line (SaOS-2) behaviour. Diatomaceous earth and silica, together with commercial hydroxyapatite were respectively the silicon and HA sources used to produce the Si-HA coatings. HA coatings with 0 wt% of silicon were used as control of the experiment. Pulsed laser deposition (PLD) was the selected technique to deposit the coatings. The Si-HA thin films were characterized by Fourier Transformed Infrared Spectroscopy (FTIR) demonstrating the efficient transfer of Si to the HA structure. The in vitro cell culture was established to assess the cell attachment, proliferation and osteoblastic activity respectively by, Scanning Electron Microscopy (SEM), DNA and alkaline phosphatase (ALP) quantification. The SEM analysis demonstrated a similar adhesion behaviour of the cells on the tested materials and the maintenance of the typical osteoblastic morphology along the time of culture. The Si-HA coatings did not evidence any type of cytotoxic behaviour when compared with HA coatings. Moreover, both the proliferation rate and osteoblastic activity results showed a slightly better performance on the Si-HA coatings from diatoms than on the Si-HA from silica.

Growth performance parameters and selected quality traits of meat and femoral bone of broiler chickens fed diet supplemented with amorphous diatomaceous earth

“…Negative effects of a silicon-poor diet were demonstrated by Carlisle (1972) -deficiency of this element caused skeleton deformation and incorrect joint development in chickens. The effect of diatomaceous earth on the improvement of skeleton resistance and condition was also confirmed in studies conducted by Calomme et al (2002) and López-Álvarez et al (2009). Feed supplementation with silicon may, additionally, significantly affect the appropriate development of young organisms, which was proved by Carlisle (1972) -one-day cockerels fed a diet based on amino acids were later characterized by inhibited growth and development, whereas those administered a feed mixture supplemented with silicon were characterized by a 50% higher growth rate and normal development.…”

Novel selenium‐doped hydroxyapatite coatings for biomedical applications

“…2 Among them, PLD has proven to be a very versatile technique that allows a finer control in the composition and morphology of the deposited material with improved adherence. [2][3][4][5][6] New requirements of HA coatings with higher functionality have led to the development of new high standard materials with compositions more similar to the natural bone apatites. It is well known that biological apatite contains additionally significant amounts of ionic substitutions, such as potassium, sodium or carbonate groups, that contribute to different metabolic routes affecting directly to the functionality of the bone tissue.…”

Effects of acid hydrolysis on microstructure of cured urea‐formaldehyde resins using atomic force microscopy

“…Different methods frequently used to modify metallic surfaces, as plasma‐spraying, magnetron sputtering, ion beam coating, electrophoretic deposition or pulsed laser deposition (PLD), are being intensively investigated especially focused in the obtaining of HA bioactive coatings 2. Among them, PLD has proven to be a very versatile technique that allows a finer control in the composition and morphology of the deposited material with improved adherence 2–6…”