Silicon supported lipid–DNA thin film structures at varying temperature studied by energy dispersive X-ray diffraction and neutron reflectivity

Domenici, Fabio; Castellano, Carlo; Dell'Unto, F; Albinati, Alberto; Congiu, A.

doi:10.1016/j.colsurfb.2011.07.026

Cited by 4 publications

(6 citation statements)

References 45 publications

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“…In particular, the hydrophobic interactions occurring at the lipid interface characteristic of skin is expected to accelerate their applications. 20 Progress is being made to resolve the complex structure of biological membrane, for example Neutron Reflectivity (NR) studies of supported multi-layer lipid structures and their structural change as a result of inclusion of DNA have been reported 21 . Other novel techniques such as combination of Molecular Dynamic simulations and Neutron Reflectivity for characterization of lipid bilayers have also been recently reported 22 .…”

Section: Introductionmentioning

confidence: 99%

Interaction of thermal responsive NIPAM nanogels with model lipid monolayers at the air-water interface

Sun

Resmini

Zarbakhsh

2018

Journal of Colloid and Interface Science

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Understanding the interaction of nanoparticles (NP) with ceramide lipids is important in developing strategies to overcome the formidable obstacle that is skin. This paper presents studies of interactions between N-isopropylacrylamide nanogels, crosslinked with 30% N,N'-methylenebisacrylamide, and model ceramide lipid monolayers at the air-water interface as a function of temperature. In the case of the mixed ceramide/cholesterol/behenic acid monolayer, the interaction of nanogels with the ceramide was strongly mediated by the fatty acids. This interaction between nanogels and monolayer components is dominated by hydrophobic-hydrophobic binding. The data show the important intermediary role of the fatty acid in facilitating transmembrane transport. For a pure ceramide lipid monolayer, the neutron reflectivity (NR), Brewster angle microscopy (BAM) and surface pressure results showed a lipid-nanogel complex formation and the subsequent depletion/solubilisation of the lipids from the interface when the area per molecule for the lipid was increased from 42 to 44 Å.

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Section: Introductionmentioning

confidence: 99%

Interaction of thermal responsive NIPAM nanogels with model lipid monolayers at the air-water interface

Sun

Resmini

Zarbakhsh

2018

Journal of Colloid and Interface Science

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“…Generally speaking, the shape and stability of lipoplexes depend largely on the chemical composition and physical properties of the lipids constituting the complex. 24 And the DNA delivery efficiency of lipoplexes is found to be mainly influenced by the features of the involved lipids. 25,26 Thus, studies regarding the lipid matrix could be crucial to find optimal lipid phases for transfection 27 and may even lead to overcome the efficiency limit of nonviral DNA delivery pathways.…”

Section: Introductionmentioning

confidence: 99%

“…25,26 Thus, studies regarding the lipid matrix could be crucial to find optimal lipid phases for transfection 27 and may even lead to overcome the efficiency limit of nonviral DNA delivery pathways. 24 For the lipid matrix, some important questions such as the structure, phase stability, phase separation, and domain or defect formation may arise. There have been several recent attempts aiming at elucidating the structure and phase state of the binary lipid mixtures.…”

Section: Introductionmentioning

confidence: 99%

Demixing and crystallization of DODAB in DPPC–DODAB binary mixtures

Sun

et al. 2014

Phys. Chem. Chem. Phys.

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The crystallization mechanism of one lipid component within multicomponent lipid mixtures remains unclear. To shed light on this issue, we studied the demixing and crystallization behaviors of a binary lipid system using neutral dipalmitoylphosphatidylcholine (DPPC) and cationic dioctadecyldimethylammonium bromide (DODAB) as model molecules. The results indicate that when DODAB is no more than equimolar (e.g., DPPC/DODAB = 2/1 and 1/1), DPPC is miscible with DODAB and hinders the crystallization of DODAB, and the samples undergo reversible gel-fluid phase transitions upon heating and cooling. However, when DODAB is dominant in the mixture (DPPC/DODAB = 1/2), cooling of the mixed fluid phase results in the formation of a DODAB-rich gel domain and a DPPC-DODAB mixed gel domain. Such phase-separated mixed gels can undergo further demixing and crystallization, producing a DODAB-rich crystalline domain and a DPPC-rich tilted gel domain upon prolonged (or plus low-temperature) incubation. Besides, evidence has been given that the crystallized DODAB-rich domain remains in the same lipid bilayer as the DPPC-rich domain. All the three binary lipid mixtures can hold large amounts of water in the lipid interlamellar regions, allowing the incorporation of a large number of water-soluble substances such as DNA or proteins, which can be used for the fabrication of functional biofilms and biomaterials. Influences of water content and salt concentration on the phase structures (e.g., repeat distances) of the binary mixtures have also been studied.

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“…85,90,92,95,96 Figure 1E points to a study by Domenici et al in which neutron and X-ray techniques are combined in a complementary manner. 16 Small angle neutron/X-ray scattering (SANS, SAXS) are related techniques where the elastic scattering by a sample which has inhomogeneities in the nanometer range is recorded at angles between, typically, 0.1 and 10°. SANS/SAXS yield information about the size, shape, and orientation of structures in the sample.…”

Section: ■ Methods Overviewmentioning

confidence: 99%

Section: Methods Overviewmentioning

confidence: 99%

Instrumental Methods to Characterize Molecular Phospholipid Films on Solid Supports

Gözen

Jesorka

2012

Anal. Chem.

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Silicon supported lipid–DNA thin film structures at varying temperature studied by energy dispersive X-ray diffraction and neutron reflectivity

Cited by 4 publications

References 45 publications

Interaction of thermal responsive NIPAM nanogels with model lipid monolayers at the air-water interface

Interaction of thermal responsive NIPAM nanogels with model lipid monolayers at the air-water interface

Demixing and crystallization of DODAB in DPPC–DODAB binary mixtures

Instrumental Methods to Characterize Molecular Phospholipid Films on Solid Supports

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