Silicon surface modification with a mixed silanes layer to immobilize proteins for biosensor with imaging ellipsometry

Abstract

One kind of surface modification method on silicon wafer was presented in this paper. A mixed silanes layer was used to modify silicon surface and rendered the surface medium hydrophobic. The mixed silanes layer contained two kinds of compounds, aminopropyltriethoxysilane (APTES) and methyltriethoxysilane (MTES). A few of APTES molecules in the layer was used to immobilize covalently human immunoglobulin G (IgG) on the silicon surface. The human IgG molecules immobilized covalently on the modified surface could retain their structures well and bind more antibody molecules than that on silicon surface modified with only APTES. This kind of surface modification method effectively improved the sensitivity of the biosensor with imaging ellipsometry.

Suppressing H2Evolution by Silicon Powder Dispersions

“…The hydrolysable part of the silane molecule is able to chemically react with hydroxyl groups present at inorganic surfaces to form a (] -O -Si -R 1 -X) hydrophobic barrier that prevents the transport of water to the coated surface [14]. The degree of hydrophobicity imparted by a particular silane to a coated surface naturally depends on the nature of the alkyl substituents [11].…”

“…They have been proposed as corrosion inhibitors for metals [10] and silicon wafers [11]. Silane coupling agents have the general structure X -R 1 -Si -(OR 2 ) 3 where -OR 2 represents a hydrolysable group capable of reacting with inorganic materials and R 1 is an alkyl chain that (optionally) is terminated by an organoreactive group X [10,12,13].…”

Nanoplasmonic Sensing using Metal Nanoparticles

“…The surface chemistry should enable an immobilization of the biorecognition elements without affecting the functionality and at the same time be resistant to nonspecific adsorption. Much work has been invested into surface chemistry development, both with 2D-structures based on for example thiols 124,125 or silanes 126 and 3D-structures like hydrogels composed of various polymers (e.g.…”

Silane-modified surfaces in specific antibody-mediated cell recognition

“…Among these technologies, silanization with organosilanes carrying chemically active www.fhc.viamedica.pl groups [8,9], such as (3-aminopropyl)triethoxysilane (APTES) ( Figure 1A), is one of the most common and simple methods. Antibodies are immobilized on modified silica surfaces by forming covalent bonds between protein functional groups and the complementary coupling groups of organosilane [10,11]. APTES can accommodate these molecules in an appropriate orientation so that their functionality and binding activity are essentially retained [8,12].…”