A scalable
method for the assembly of oriented bacterial cellulose
(BC) films is presented based on using wrinkled thin silicone substrates
of meter-square size as templates during biotechnological syntheses
of BC. Control samples, including flat templated and template-free
bacterial cellulose, along with the oriented BC, are morphologically
characterized using scanning electron microscopy (SEM). Multiple functional
properties including wettability, birefringence, mechanical strength,
crystallinity, water retention, thermal stability, etc., are being characterized for the BC samples, where the wrinkling-induced
in situ BC alignment not only significantly improved material mechanical
properties (both strength and toughness) but also endowed unique material
surface characteristics such as wettability, crystallinity, and thermal
stability. Owing to the enhanced properties observed, potential applications
of wrinkle templated BC in printing and cell culture are being demonstrated
as a proof of concept, which renders their approach promising for
various biomedical and packaging applications.