Silk fibers assisted long-term 3D culture of human primary urinary stem cells via inhibition of senescence-associated genes: Potential use in the assessment of chronic mitochondrial toxicity

Ding, Huifen; George, Sunil; Leng, Xiaoyan; Ihnat, Michael A.; Ma, Jian-Xing; Jiang, Guochun; Dumond, Julie B.; Zhang, Yuanyuan

doi:10.1016/j.mtadv.2022.100261

Cited by 10 publications

(9 citation statements)

References 96 publications

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“…[1][2][3] These perovskite solar cells have been mainly fabricated via solution processes, ranging from lab-scale spin-coating 4 to large-scale slot-die coating, 5 exhibiting a record power conversion efficiency of 25.7% and a T80 lifetime of over 2000 hours. [6][7][8][9] However, challenges remain for solution-processed perovskite solar cells, particularly during large-scale production, including precise control of precursor solutions, minimizing the toxicity of solvents as well as understanding the complexity of crystalline processes. [10][11][12] Along with solution processes, vapor deposition has the advantages of high potential for large-area modules, facile integration with tandem solar cells, and compatibility with existing industrial manufacturing techniques, benecial for industrial-scale fabrication.…”

Section: Introductionmentioning

confidence: 99%

Advancing vapor-deposited perovskite solar cellsviamachine learning

Wang

Zheng

et al. 2023

J. Mater. Chem. A

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Section: Introductionmentioning

confidence: 99%

Advancing vapor-deposited perovskite solar cellsviamachine learning

Wang

Zheng

et al. 2023

J. Mater. Chem. A

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“…Specifically, 10% MTT-containing medium was used for the 2D dish culture, whereas 10% CCK8-containing medium was used for evaluating the cell viability in 3D hydrogel or spheroids. [6,57,58] For MTT analysis, after 4 h incubation, half of the supernatant was removed from each well, and an equivalent volume of DMSO was added. Following an additional 10 min of incubation, absorbance was measured at 570 nm.…”

Section: Methodsmentioning

confidence: 99%

Self‐Healing Hydrogel Containing Decellularized Liver Matrix and Endothelial Cell‐Covered Hepatocyte Spheroids for Rescue of Injured Hepatocytes

Chou,

Cheng,

Yin

et al. 2024

Macromolecular Bioscience

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Liver fibrosis occurs in many chronic liver diseases, while severe fibrosis can lead to liver failure. A chitosan‐phenol based self‐healing hydrogel (CP) integrated with decellularized liver matrix (DLM) is proposed in this study as a 3D gel matrix to carry hepatocytes for possible therapy of liver fibrosis. To mimic the physiological liver microenvironment, DLM is extracted from pigs and mixed with CP hydrogel to generate DLM‐CP self‐healing hydrogel. Hepatocyte spheroids coated with endothelial cells (ECs) are fabricated using a customized method and embedded in the hydrogel. Hepatocytes injured by exposure to CCl4‐containing medium are used as the in vitro toxin‐mediated liver fibrosis model, where the EC‐covered hepatocyte spheroids embedded in the hydrogel are co‐cultured with the injured hepatocytes. The urea synthesis of the injured hepatocytes reaches 91% of the normal level after 7 days of co‐culture, indicating that the hepatic function of injured hepatocytes is rescued by the hybrid spheroid‐laden DLM‐CP hydrogel. Moreover, the relative lactate dehydrogenase activity of the injured hepatocytes is decreased 49% by the hybrid spheroid‐laden DLM‐CP hydrogel after 7 days of co‐culture, suggesting reduced damage in the injured hepatocytes. The combination of hepatocyte/EC hybrid spheroids and DLM‐CP hydrogel presents a promising therapeutic strategy for hepatic fibrosis.

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“…Precision nephrotoxicity testing aims to overcome these limitations by leveraging cutting-edge technologies such as genomics, proteomics, metabolomics and imaging to develop more accurate and sensitive biomarkers. In this review, we discuss precision nephrotoxicity testing using primary human renal cells in 3D in vitro models as a method for predicting druginduced kidney injury [6][7][8][9].…”

Section: Precision Nephrotoxicity Testingmentioning

confidence: 99%

“…3D models more closely mirror cellular architecture [9,11], gene expression patterns [12], oxygen and nutrient diffusion dynamics [13], than 2D culture models. Drugs known to affect microarchitecture, mitochondrial function, and alter gene expression, (i.e., oxygen consumption rate/extracellular acidification rate [OCR/ECAR], mitochondrial membrane potential [MMP], adenosine triphosphate [ATP] production, complex I-V expression and activity, mitochondrial morphology, DNA content-Mitochondrial [mtDNA] content, apoptosis, and reactive oxygen species [ROS]) demonstrate better physiological toxicity in 3D vs 2D models [8,9]. These provide a more realistic representation of in vivo physiology and better predict drug toxicity compared to 2D cultures.…”

Section: Precision Nephrotoxicity Testingmentioning

confidence: 99%

“…USCs form functional tubular structures in 3D cultures. These properties make USCs a valuable cell source for the development of 3D in vitro models for evaluating drug-induced nephrotoxicity [6,7] and renal mitochondrial toxicity [8,9]. This review specifically focuses on precision nephrotoxicity testing using human renal cells, particularly USCs, in 3D in vitro models.…”

Section: Precision Nephrotoxicity Testingmentioning

confidence: 99%

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Precision nephrotoxicity testing using 3D in vitro models

Yu,

Zhu,

Bosholm

et al. 2023

Cell Biosci

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Nephrotoxicity is a significant concern during the development of new drugs or when assessing the safety of chemicals in consumer products. Traditional methods for testing nephrotoxicity involve animal models or 2D in vitro cell cultures, the latter of which lack the complexity and functionality of the human kidney. 3D in vitro models are created by culturing human primary kidney cells derived from urine in a 3D microenvironment that mimics the fluid shear stresses of the kidney. Thus, 3D in vitro models provide more accurate and reliable predictions of human nephrotoxicity compared to existing 2D models. In this review, we focus on precision nephrotoxicity testing using 3D in vitro models with human autologous urine-derived kidney cells as a promising approach for evaluating drug safety.

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Silk fibers assisted long-term 3D culture of human primary urinary stem cells via inhibition of senescence-associated genes: Potential use in the assessment of chronic mitochondrial toxicity

Cited by 10 publications

References 96 publications

Advancing vapor-deposited perovskite solar cellsviamachine learning

Advancing vapor-deposited perovskite solar cellsviamachine learning

Self‐Healing Hydrogel Containing Decellularized Liver Matrix and Endothelial Cell‐Covered Hepatocyte Spheroids for Rescue of Injured Hepatocytes

Precision nephrotoxicity testing using 3D in vitro models

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