Silk Fibroin as an Additive for Cell-Free Protein Synthesis

Lee, Marilyn S.; Hung, Chia-Suei; Phillips, Daniel A.; Buck, Chelsea C.; Gupta, Maneesh K.; Lux, Matthew W.

doi:10.1101/2020.05.28.121616

Cited by 2 publications

(2 citation statements)

References 48 publications

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“…The majority of methods complete the extract preparation by aliquoting, flash-freezing in liquid nitrogen, and storage at −80 °C. However, recent studies have explored lyophilization as a storage method, including optimizing lyophilization protocols [ 25 , 128 ], separate processing of extract and reagent mix [ [129] , [130] , [131] ], use of cryoprotectants [ 129 , 131 , 132 ], and even as a combination storage and lysis step [ 14 ]. Robust performance after storage at elevated temperatures of 37 °C for several months has been shown [ 129 ].…”

Section: Methods Of Extract Preparation For E Colimentioning

confidence: 99%

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Cole¹,

Miklos²,

Chiao³

et al. 2020

Synthetic and Systems Biotechnology

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Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review of cell-extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the diversity of Escherichia coli genetic strains available and their corresponding utility. We then trace the history of cell-extract methodology over the past 20 years, showing key improvements that lower the entry level for new researchers. Next, we survey the rise of new prokaryotic cell-free systems, with associated methods, and the opportunities provided. Finally, we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible.

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Section: Methods Of Extract Preparation For E Colimentioning

confidence: 99%

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Cole¹,

Miklos²,

Chiao³

et al. 2020

Synthetic and Systems Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…EG needs in modern society It has increased to an unprecedented level, and the continuous combination of modern BT and the environment has played a very important role in my country's EG and protection work, and has gradually developed into an important service and support method that my country's ecological field relies on. On the basis of existing EG and protection measures, a new type of environmental information technology is formed, which lays a foundation for EG and protection, and shows irreplaceable EV [5][6].…”

Section: The Environmental Governance (Eg) Value Of Modern Btmentioning

confidence: 99%

Ecological Environment and Pollution Control under the Influence of Modern Biotechnology Development

2022

AJEB

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Modern biotechnology (BT) has EV. On the one hand, by giving full play to the role of technology, the relationship between man and nature can be better handled and the two can coexist harmoniously; on the other hand, if problems have arisen in the ecological environment, it is necessary to use existing technologies to prevent ecological imbalances phenomenon to repair, thereby making sustainable development possible. The main purpose of this paper is to analyze and study the impact of the development of modern BT on the ecological environment and pollution control. This paper will focus on the EV of modern BT from three aspects: ecological manufacturing, EG and environmental restoration. In this paper, the screened biological indicators (Neu%, Eos%, Eos#, Lym%, IL-6, IL-10 and CSF) were calculated by comprehensive index. The experimental results found that no matter the average, maximum or minimum values, there were the comprehensive index of biological samples in the high pollution exposure group > the comprehensive index of biological samples in the low pollution exposure group > the comprehensive index of biological samples in the no pollution exposure group.

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Silk Fibroin as an Additive for Cell-Free Protein Synthesis

Cited by 2 publications

References 48 publications

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Ecological Environment and Pollution Control under the Influence of Modern Biotechnology Development

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