Silk fibroin-based vascular repairing sheet with angiogenic-promoting activity of SVVYGLR peptide regenerated the damaged vascular in rats

Abstract

Medical sheets are useful in surgically repair vascular disease. To avoid long-term side effects, they are to be replaced with regenerated tissue after implantation. Silk fibroin is a fibrous protein secreted by silkworm. The advantage of silk fibroin is its biocompatibility and has been used as regenerative artificial materials. The problem of its biodegradability is that the effect is time consuming. In this study, SVVYGLR peptide was used to expect promoting cell migration and accelerating the biodegradation of silk fibroin. Silk fibroin and polyurethane-based medical sheets with or without SVVYGLR peptide were implanted in rat abdominal aorta (silk fibroin/polyurethane/SVVYGLR peptide versus silk fibroin/polyurethane). The result of histological evaluation indicated that the new cell layer created under both sheets was composed of endothelial cells, smooth muscle, and fibroin in both sheets and similar to a native vessel. Both sheets did not show any excessive inflammation or calcification, and moderate biodegradability was observed. The decrease of silk fibroin indicated the biodegradability of all sheets. Silk fibroin/polyurethane/SVVYGLR peptide had many small vessels in the regenerated tissue than silk fibroin/polyurethane. This appearance indicated that SVVYGLR peptide promoted the angiogenesis in the regenerative tissue. This study suggested that SVVYGLR peptide could give the angiogenic-promoting activity to silk fibroin-based vascular repairing sheet.

Applications of Silk Fibroin in Human and Veterinary Medicine

“…To compare the ability of the SVVYGLR peptide to induce angiogenesis, a transplant was also performed using only SF with polyurethane. Despite similar cellular composition in both cases, more advanced angiogenesis was found in the presence of the SVVYGLR peptide [69].…”

“…Reducing the time needed for biodegradation makes it possible to prevent the occurrence of side effects of the transplant, such as an inflammatory reaction. The migration of epithelial cells as well as smooth muscle cells occurs faster, which contributes to a faster similarity of the graft to a natural blood vessel [69].…”

“…Protein/Polymer Advantages bone collagen and decellularised ECM good environment for bone cells proliferation obtained by low temperature print [41,43] collagen, HA and recombinant human erythropoietin gradual degradation and fostering of the osteoblasts proliferation in scaffolds obtained by the low-temperature print [20] poly(ε-caprolactone) and chitosan as two-layers mats enhanced biocompatible and antibacterial properties, sufficient hydrophilicity, high tear strength and other mechanical properties improved [59] chitosan as film regulation of cytokine and GF secretion and stimulation of cell proliferation and differentiation [58] skin sericin enhancement of cell proliferation and antimicrobial properties [29] antibiotics (e.g., polymyxin) limit of microbial activity [12] blood vessels Sil-MA as hydrogel high patency rate of small diameter vessels [43] melanin nanoparticles improvement of printed material quality [43] PET promising material for grafts for small diameter blood vessels [68] polyurethane and SVVYGLR peptide angiogenic-promoting activity [69] In conclusion, silk, which has historically held an undisputed position in the textile industry, can now find many other applications. Its biocompatibility, strength and versatility make silk a valuable material for a variety of medical applications, such as implantology, tissue engineering, wound dressings and drug delivery, all of which are discussed in this article.…”

Hydrogel Tissue Bioengineered Scaffolds in Bone Repair: A Review

“…SF is one of the most popular biopolymers for tissue bioengineering scaffolds and holds great promise for tissue engineering applications. According to a previous study, the incorporation of SF can promote the wound repair ability of wound dressings, cell proliferation, and angiogenesis in damaged tissues [150]. These advantages of SF are also good improvements for repairing bone defects.…”

Clinical Application for Tissue Engineering Focused on Materials

“…When a nonwoven fabric made by mixing silk fibroin and PU with SVVYGLR peptides with angiogenesis ability was buried in the rat abdominal aorta, many minute neovascular vessels were confirmed, in addition to the decomposition of materials and the formation of endothelial and smooth muscle layers, in which tissue infiltration and vascular neogenesis were similar to native blood vessels. These strategies affect the immediate effect and the longer-term performance [ 44 , 45 ].…”