Silk fibroin/calcium alginate composite modifying supermacroporous molecularly imprinted membrane synthesis for high performance on recognizing bovine hemoglobin

Abstract

Bio‐macromolecule hemoglobin (Hb) is a kind of iron‐rich metalloprotein that abundant in erythrocytes with various physiological properties. Separating Hb from bio‐matrix with high efficacy is crucial to Hb application but quite challenging. In this study, a supermacroporous molecularly imprinted membrane (MIM) was prepared with high performance on recognizing bovine hemoglobin (BHb). By using the silk fibroin and calcium alginate as mechanical modifiers, the mechanical strength of the prepared membrane was significantly improved to at around 0.25 MPa, and the supermacroporous structure in MIM was also formed. The MIM adsorption capacity of BHb was as high as 121.8 mg g−1 at the initial concentration of 0.5 mg mL−1. The MIM separation factor of BHb from BHb/BSA mixtures was as high as 7.34, and the relative imprinting factor was at around 4.0. The prepared MIM demonstrated the superiority and the valuable application prospect on specific separation of BHb.

Selective Extraction and Quantification of Hemoglobin Based on a Novel Molecularly Imprinted Nanopolymeric Structure of Poly(acrylamide-vinyl imidazole)

“…The detection of Hb from blood samples using molecularly imprinted polymers (MIPs) involves the creation of selective and specific binding sites within the polymer matrix that are designed to recognize and capture Hb molecules. − MIPs can be intricately designed to distinctly identify and attach to Hb, thereby reducing interference from other constituents in the blood sample. ,, MIPs demonstrate the advantageous quality of reusability, allowing them to be employed for numerous detection cycles . This characteristic contributes to cost-effectiveness and resource efficiency . MIPs commonly showcase robust stability, rendering them well-suited for practical applications.…”

“… 51 This characteristic contributes to cost-effectiveness and resource efficiency. 52 MIPs commonly showcase robust stability, rendering them well-suited for practical applications. This inherent stability enhances the reliability and longevity of MIPs in the context of Hb detection, further solidifying their utility in various analytical settings.…”