Silk Fibroin Films for Corneal Endothelial Regeneration: Transplant in a Rabbit Descemet Membrane Endothelial Keratoplasty

Vázquez, Natalia; Rodríguez-Barrientos, Carlos A.; Aznar-Cervantes, Salvador D.; Chacón, Manuel; Cenis, J. L.; Riestra, Ana Cristina; Sánchez-Ávila, Ronald Mauricio; Persinal, Mairobi; Brea-Pastor, Agustín; Cueto, Luis Fernández-Vega; Meana, Álvaro; Merayo‐Lloves, Jesús

doi:10.1167/iovs.17-21797

Cited by 51 publications

(51 citation statements)

References 59 publications

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“…); therefore, foreign body responses to silk-based implants and sutures might be expected. Various studies performed in rodents and large animals to evaluate silk fibroin biocompatibility across many different formats, including meshes ( 52 ), thin films ( 19 , 20 ), hydrogels ( 27 , 45 , 62 ), nanoparticles ( 63 – 65 ) and liquid solutions ( 23 , 25 ), have confirmed that silk fibroin induces no significant adverse responses, thereby substantiating the claims that silk fibroin is a biocompatible biopolymer. More importantly, these observations translate well to humans ( 1 ).…”

Section: Introductionmentioning

confidence: 72%

“… Vazquez et al . ( 20 ) B. mori ; 30 min; water-annealed under vacuum 24 h; sterilized 70% EtOH; silk film Descemet membrane replacement; substrate for human and rabbit corneal endothelial cells In vitro and in vivo , rabbits; tested transparency, gene expression, cell growth, integration and proliferation; no signs of immune rejection. Applegate et al .…”

Section: Introductionmentioning

confidence: 99%

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A Review of the Emerging Role of Silk for the Treatment of the Eye

2018

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Silk is a remarkable biopolymer with a long history of medical use. Silk fabrications have a robust track record for load-bearing applications, including surgical threads and meshes, which are clinically approved for use in humans. The progression of top-down and bottom-up engineering approaches using silk as the basis of a drug delivery or cell-loaded matrix helped to re-ignite interest in this ancient material. This review comprehensively summarises the current applications of silk for tissue engineering and drug delivery, with specific reference to the eye. Additionally, the review also covers emerging trends for the use of silk as a biologically active biopolymer for the treatment of eye disorders. The review concludes with future capabilities of silk to contribute to advanced, electronically-enhanced ocular drug delivery concepts.

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Section: Introductionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

A Review of the Emerging Role of Silk for the Treatment of the Eye

2018

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“…Although previous studies have revealed the favourable biocompatibility of silk fibroin from B. mori and its excellent properties for use as a biomaterial in tissue engineering applications 39 – 41 , no cytocompatibility or biocompatibility studies are available for Tl- S, nor for any silk derived from mites. To test Tl- S cytocompatibility, we performed a study with silk films and nanoparticles of T. lintearius using the murine fibroblast cell line L929, which is commonly used in the evaluation of cytocompatibility and cytotoxicity of potential biomedical devices 42 .…”

Section: Resultsmentioning

confidence: 99%

The silk of gorse spider mite Tetranychus lintearius represents a novel natural source of nanoparticles and biomaterials

Lozano-Pérez¹,

Pagán²,

Zhurov

et al. 2020

Sci Rep

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Spider mites constitute an assemblage of well-known pests in agriculture, but are less known for their ability to spin silk of nanoscale diameters and high Young’s moduli. Here, we characterize silk of the gorse spider mite Tetranychus lintearius, which produces copious amounts of silk with nano-dimensions. We determined biophysical characteristics of the silk fibres and manufactured nanoparticles and biofilm derived from native silk. We determined silk structure using attenuated total reflectance Fourier transform infrared spectroscopy, and characterized silk nanoparticles using field emission scanning electron microscopy. Comparative studies using T. lintearius and silkworm silk nanoparticles and biofilm demonstrated that spider mite silk supports mammalian cell growth in vitro and that fluorescently labelled nanoparticles can enter cell cytoplasm. The potential for cytocompatibility demonstrated by this study, together with the prospect of recombinant silk production, opens a new avenue for biomedical application of this little-known silk.

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“…The study demonstrated good support for the growth of human and rabbit corneal endothelial cells with the appropriate morphology and in vivo functionality after surgical implantation. [48] A fully synthetic hydrogel based on poly(ethylene glycol) and poly(caprolactone) (PCL) that are cross-linked with sebacic acid (Figure 7) has been studied as a substrate for corneal endothelial cell transplantation. [49] It has been shown to support excellent primary sheep corneal endothelial cell growth and monolayer formation with functional behavior after 7 d. Films of 50 µm thickness could be produced which had sufficient mechanical properties for surgical implantation into sheep eyes and this preliminary in vivo study demonstrated no adverse inflammatory response to the films with or without cells after 28 d. A similar in vitro response has been observed using a synthetic peptide based on poly(ε-lysine) cross-linked with nonanedioic acid with primary pig corneal endothelial cells (Figure 8).…”

Section: Endotheliummentioning

confidence: 99%

Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

Williams

Lace

Kennedy

et al. 2018

Adv Healthcare Materials

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The role of biomaterials in tissue engineering and regenerative medicine strategies to treat vision loss associated with damage to tissues in the anterior segment of the eye has been studied for several years. This has mostly involved replacement and support for the cornea and conjunctiva. These are complex tissues with specific functional requirements for different parts of the tissue. Amniotic membrane (AM) is used in clinical practice to transplant autologous or allogenic cells to the corneal surface. Fibrin gels have also progressed to clinical use under specific conditions. Alternatives to AM such as collagen gels, other natural materials, for example keratin and silks, and synthetic polymers have received considerable attention in laboratory and animal studies. This experience is building a body of evidence to demonstrate the potential of tissue engineering and regenerative medicine in corneal and conjunctival reconstruction and can also lead to other applications in the anterior segment of the eye, for example, the trabecular meshwork. There is a real clinical need for new procedures to overcome vision loss but there are also opportunities for developments in ocular applications to lead to biomaterials innovations for use in other clinical areas.

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Silk Fibroin Films for Corneal Endothelial Regeneration: Transplant in a Rabbit Descemet Membrane Endothelial Keratoplasty

Cited by 51 publications

References 59 publications

A Review of the Emerging Role of Silk for the Treatment of the Eye

A Review of the Emerging Role of Silk for the Treatment of the Eye

The silk of gorse spider mite Tetranychus lintearius represents a novel natural source of nanoparticles and biomaterials

Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

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