Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

Bioactive Scaffolds for the Controlled Formation of Complex Skeletal Tissues

“…These interactions trigger signaling cascades that could result in the observed capacity of hyaluronan to instruct tissue neoformation and regeneration. For illustration, hyaluronan has shown pro-proliferative properties (Zou et al, 2004), capacity to enhance unspecific ECM deposition (Garcia-Fuentes et al, 2009), to induce cartilage (Allemann et al, 2001;Williams et al, 2003;Yamane et al, 2005) and ligament (Cristino et al, 2005) formation, capacity to maintain embryonic stem cells in undifferentiated state (Gerecht et al, 2007), and capacity to modulate inflammatory and catabolic markers Homandberg et al, 2004). Hyaluronan biological properties, though, seem to be very dependent on its molecular weight, and the presence of hyaluronan oligosaccharides have been connected to chondrocyte-driven chondrolysis (Knudson et al, 2000).…”

Bioreactor Design for Tendon/Ligament Engineering

“…In the past decade, promising results have been achieved using mesenchymal stem cells (MSCs) in tendon/ligament engineering. [16][17][18][19][20][21][22][23] However, the potential for MSCs to also differentiate into osteoblastic cells leading to ectopic bone formation raises concerns for their application in tendon repair. Likewise, bone marrow-derived MSCs that have been used in various studies [24][25][26][27][28] also pose a potential risk of ectopic bone formation.…”

Effect of Starch on Property of Silk Fibroin/Keratin Blend Films

“…Silk component, fibroin, has been reported as an important biomaterial which can be constructed in various forms [4]. In addition, silk fibroin has been blended with other materials to obtain novel materials such as collagen [5] PLGA and alginate [6] or hyaluronan [7]. Keratin, a kind of high cystein content fibrous protein, has been applied in various fields include tissue engineering [8].…”