Silk fibroin mediated delivery of liposomal emodin to breast cancer cells

Cheema, Sangeeta K.; Gobin, Andrea S.; Rhea, Robyn; López-Berestein, Gabriel; Newman, Robert A.; Mathur, Anshu B.

doi:10.1016/j.ijpharm.2007.03.043

Cited by 65 publications

(37 citation statements)

References 41 publications

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“…In this study, the higher SF content (10% w/v) resulted in higher uptake and intracellular residence time for curcumin, which also increased efficacy against breast cancer cells. While the SF coating of emodin in the study by Cheema and colleagues was 0.1% w/v SF, 14 we found that increasing the SF coating amount from 0.1% w/v to 10% w/v increased the curcumin entrapment and cellular uptake significantly, thereby affecting efficacy.…”

Section: Dovepresscontrasting

confidence: 45%

“…Since curcumin is known to act through many pathways in various cancer cells, 2 there may be a down modulation of more than one pathway in breast cancer cells due to longer availability of curcumin via SF nanoparticles. 14 One of the limitations of this study was the minor drug loss during the transfer of nanoparticles from the glass slides. Although the use of the same procedure to transfer the nanoparticles should have minimized this effect when comparing groups.…”

Section: Dovepressmentioning

confidence: 94%

“…Silk microspheres, 10,11 nanolayers, 12 and coatings on drug-loaded liposomes 13,14 were previously used for the controlled release of various other drugs. Previously, silk fibroin (SF)-coated liposomal emodin was shown to have higher efficacy against breast cancer cells as compared to the uncoated liposomal emodin due to increased retention of emodin in the presence of SF.…”

Section: Dovepressmentioning

confidence: 99%

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Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy

Gupta

Aseh

Ríos

et al. 2009

IJN

246

118

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Biologically derived nanoparticles (100 nm) were fabricated for local and sustained therapeutic curcumin delivery to cancer cells. Silk fibroin (SF) and chitosan (CS) polymers were blended noncovalently to encapsulate curcumin in various proportions of SF and CS (75:25, 50:50, and 25:75 SF:CS) or pure SF at two concentrations (0.1% w/v and 10% w/v) using the devised capillary-microdot technique. Curcumin-polymer conjugates were frozen, lyophilized, crystallized, suspended in phosphate-buffered saline for characterization, and tested for efficacy against breast cancer cells. All nanoparticle formulations except 0.1% w/v 50:50 SFCS were less than 100 nm in size as determined with the transmission electron microscopy. The entrapment and release of curcumin over eight days was highest for SF-derived nanoparticles as compared to all SFCS blends. The uptake and efficacy of SF-coated curcumin was significantly higher (p  0.001) than SFCS-coated curcumin in both low and high Her2/neu expressing breast cancer cells. Interestingly, the uptake of curcumin was highest for the high Her2/neu expressing breast cancer cells when delivered with a 10% w/v SF coating as compared to other formulations. In conclusion, SF-derived curcumin nanoparticles show higher efficacy against breast cancer cells and have the potential to treat in vivo breast tumors by local, sustained, and long-term therapeutic delivery as a biodegradable system.

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Section: Dovepresscontrasting

confidence: 45%

Section: Dovepressmentioning

confidence: 94%

Section: Dovepressmentioning

confidence: 99%

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Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy

Gupta

Aseh

Ríos

et al. 2009

IJN

246

118

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“…As a consequence, neither fibroin nor its byproducts produce an inflammatory reaction or an adverse effect. 14 Besides, fibroin is not only an inert drug carrier. The peptides resulting from its hydrolysis have shown therapeutic potential, for instance, in the stimulation of glucose transport in normal and insulin-resistant 3T3-L1 adipocytes 15 or in the proliferation of fibroblasts in wound healing models.…”

mentioning

confidence: 99%

Silk fibroin nanoparticles constitute a vector for controlled release of resveratrol in an experimental model of inflammatory bowel disease in rats

Lozano-Pérez¹,

Rodríguez-Nogales²,

Ortiz-Cullera³

et al. 2014

IJN

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Purpose We aimed to evaluate the intestinal anti-inflammatory properties of silk fibroin nanoparticles, around 100 nm in size, when loaded with the stilbene compound resveratrol, in an experimental model of rat colitis. Methods Nanoparticles were loaded with resveratrol by adsorption. The biological effects of the resveratrol-loaded nanoparticles were tested both in vitro, in a cell culture of RAW 264.7 cells (mouse macrophages), and in vivo, in the trinitrobenzenesulfonic acid model of rat colitis, when administered intracolonically. Results The resveratrol liberation in 1× phosphate-buffered saline (PBS; pH 7.4) was characterized by fast liberation, reaching the solubility limit in 3 hours, which was maintained over a period of 80 hours. The in vitro assays revealed immunomodulatory properties exerted by these resveratrol-loaded nanoparticles since they promoted macrophage activity in basal conditions and inhibited this activity when stimulated with lipopolysaccharide. The in vivo experiments showed that after evaluation of the macroscopic symptoms, inflammatory markers, and intestinal barrier function, the fibroin nanoparticles loaded with resveratrol had a better effect than the single treatments, being similar to that produced by the glucocorticoid dexamethasone. Conclusion Silk fibroin nanoparticles constitute an attractive strategy for the controlled release of resveratrol, showing immunomodulatory properties and intestinal anti-inflammatory effects.

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“…Because of the controlled release of a drug by liposomes, therapeutic effects can be achieved in low doses with the encapsulated drugs. In this way, toxic effects can be either reduced or discarded, and the half-life of a drug can be prolonged (3)(4)(5)(6).…”

mentioning

confidence: 99%

Release Pattern of Liposomal Bupivacaine in Artificial Cerebrospinal Fluid

Duzlu¹,

Günaydın²,

Şüküroğlu³

et al. 2016

Turk J Anaesth Reanim

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Amaç: İn vitro koşullarda beyin omurilik sıvısı (BOS) içinde multilameller lipozomal bupivakain formülasyonlarının kontrollü salınım profilini, lipozomsuz bupivakain formlarıyla karşılaştırmayı amaçladık. Methods: Liposome formulations were prepared using a dry-film hydration method. Then, an artificial CSF-buffered solution was prepared. Bupivacaine base with liposomal bupivacaine base, bupivacaine HCl with liposomal bupivacaine HCl and bupivacaine HCl were added in a Franz diffusion cell. These solutions were kept in a hot water bath for 24 h. The samples were taken at 0.5, 1, 3, 6, 12 and 24 h (1 st series of experiment). Solutions of bupivacaine base with liposomal bupivacaine base and bupivacaine HCl with liposomal bupivacaine HCl were centrifuged to obtain liposomal bupivacaine base and liposomal bupivacaine HCl. Afterwards, liposomal bupivacaine base and liposomal bupivacaine HCl were added in a Franz diffusion cell. After keeping these solutions in a hot water bath for 24 h as well, the samples were taken at the same time intervals (2 nd series of experiment). All samples (54 from the 1st experiment and 36 from the 2 nd experiment) were analysed with high-performance liquid chromatography and ultra-performance liquid chromatography and their chromatograms were obtained. Results:After obtaining calibration curves for bupivacaine base and HCl, release patterns of these formulations were plotted. A markedly controlled slow-release pattern was observed for multilamellar liposomal bupivacaine than for non-liposomal bupivacaine in artificial CSF. Conclusion:Demonstration of controlled slow-release profile for mutilamellar liposomal bupivacaine in artificial CSF in vitro might support intrathecal use of liposomal bupivacaine in vivo in animal studies.Keywords: Liposome, bupivacaine, cerebrospinal fluid, controlled slow release Abstract / Öz

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Silk fibroin mediated delivery of liposomal emodin to breast cancer cells

Cited by 65 publications

References 41 publications

Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy

Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy

Silk fibroin nanoparticles constitute a vector for controlled release of resveratrol in an experimental model of inflammatory bowel disease in rats

Release Pattern of Liposomal Bupivacaine in Artificial Cerebrospinal Fluid

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Silk fibroin mediated delivery of liposomal emodin to breast cancer cells

Cited by 65 publications

References 41 publications

Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy

Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy

Silk fibroin nanoparticles constitute a&nbsp;vector for controlled release of resveratrol in&nbsp;an&nbsp;experimental model of inflammatory bowel&nbsp;disease in rats

Release Pattern of Liposomal Bupivacaine in Artificial Cerebrospinal Fluid

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Silk fibroin nanoparticles constitute a vector for controlled release of resveratrol in an experimental model of inflammatory bowel disease in rats