Silk fibroin photo-lyogels containing microchannels as a biomaterial platform for <i>in situ</i> tissue engineering

Baptista, Marissa; Joukhdar, Habib; Alcala-Orozco, Cesar R; Lau, Kieran; Jiang, Shouyuan; Cui, Xiaolin; He, Steven; Tang, Fengying; Heu, Céline; Woodfield, Tim B. F.; Lim, Khoon S.; Rnjak‐Kovacina, Jelena

doi:10.1039/d0bm01010c

Cited by 18 publications

(23 citation statements)

References 52 publications

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“…Cooling a solution from all directions, generally resulting in isotropic ice crystals, e.g., freezing solution in a standard freezer Unidirectional freezing Cooling a single surface of an insulated material, promoting ice crystal growth toward the cooling element -terminology variations include freeze-casting [13] Hydrogel Cross-linked hydrophilic polymer network that swells in water without dissolution; [25] in this review, we refer to hydrated polymer networks Sponge Frozen polymer solution that has been lyophilized (freeze-dried) and possibly cross-linked postdrying to induce water stability [12,26] Cryogel A scaffold that was cross-linked during the freezing process and either lyophilized or thawed afterward [19] Lyogel As scaffold that was cross-linked prior to freezing and was then lyophilized [27] Figure 1. Basic principles behind ice templating.…”

Section: Multidirectional Freezingmentioning

confidence: 99%

“…For example, lyogels can inherit the base physical properties of the constituent hydrogel and have the advantage of larger pores, therefore exhibiting higher potential for vascularization and tissue infiltration than the original constituent hydrogel. [27] When fabricating lyogels, the choice of cross-linker is dictated largely by the polymer properties rather than the ice templating process, due to freezing occurring once the hydrogel has set. In cryogels, on the other hand, cross-linker choice is selected based on both polymer properties and cross-linker sensitivity to subzero temperatures, since cryogels are cross-linked during the freezing step.…”

Section: Cryogels and Lyogels Novel Ice Templated Biomaterials With New Considerationsmentioning

confidence: 99%

Section: Analysis Of Ice Templated Materialsmentioning

confidence: 99%

“…(B-Lyogel) Adapted with permission. [27] Copyright 2020, Royal Society of Chemistry. therefore cannot provide information on pore structure distribution (anisotropic vs isotropic), lamellae thickness, and interconnectivity.…”

Section: Analysis Of Ice Templated Materialsmentioning

confidence: 99%

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Ice Templating Soft Matter: Fundamental Principles and Fabrication Approaches to Tailor Pore Structure and Morphology and Their Biomedical Applications

et al. 2021

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Porous scaffolds are widely used in biomedical applications where pore size and morphology influence a range of biological processes, including mass transfer of solutes, cellular interactions and organization, immune responses, and tissue vascularization, as well as drug delivery from biomaterials. Ice templating, one of the most widely utilized techniques for the fabrication of porous materials, allows control over pore morphology by controlling ice formation in a suspension of solutes. By fine-tuning freezing and solute parameters, ice templating can be used to incorporate pores with tunable morphological features into a wide range of materials using a simple, accessible, and scalable process. While soft matter is widely ice templated for biomedical applications and includes commercial and clinical products, the principles underpinning its ice templating are not reviewed as well as their inorganic counterparts. This review describes and critically evaluates fundamental principles, fabrication and characterization approaches, and biomedical applications of ice templating in polymer-based biomaterials. It describes the utility of porous scaffolds in biomedical applications, highlighting biological mechanisms impacted by pore features, outlines the physical and thermodynamic mechanisms underpinning ice templating, describes common fabrication setups, critically evaluates complexities of ice templating specific to polymers, and discusses future directions in this field.

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Section: Multidirectional Freezingmentioning

confidence: 99%

Section: Cryogels and Lyogels Novel Ice Templated Biomaterials With New Considerationsmentioning

confidence: 99%

Section: Analysis Of Ice Templated Materialsmentioning

confidence: 99%

Section: Analysis Of Ice Templated Materialsmentioning

confidence: 99%

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Ice Templating Soft Matter: Fundamental Principles and Fabrication Approaches to Tailor Pore Structure and Morphology and Their Biomedical Applications

et al. 2021

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“…Pore size was measured using at least eight random areas from each sample (two per day point) using ImageJ software (NIH, Bethesda) as previously described. [23] 2.6 Cell Culture and Spheroid Formation Different cells were cultured and used: i) adult human cardiac fibroblasts (hCFs) which were cultured in DMEM with 1% (v/v) Penicillin-Streptomycin (50 units/mL penicillin and 50 µg streptomycin/mL), 1% (v/v) 200 mM L-Glutamine and 10% (v/v) Fetal Bovine Serum (FBS); ii) adult human coronary artery endothelial cells (HCAEC) which were cultured in MesoEndo Cell Growth Medium (Cell Applications, Inc); and iii) Cor.4U SC-derived cardiac myocytes, which were cultured in Cor.4U media according to manufacturer's instructions (Ncardia). Spheroids were formed using a 384-hanging drop culture plate (Perfecta3D, 3D Biomatrix, Inc.) as previously described.…”

Section: Pore Size Measurements In Bioprinted Hydrogels Using Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Towards engineering heart tissues from bioprinted cardiac spheroids

et al. 2021

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Current in vivo and in vitro models fail to accurately recapitulate the human heart microenvironment for biomedical applications. This study explores the use of cardiac spheroids (CSs) to biofabricate advanced in vitro models of the human heart. CSs were created from human cardiac myocytes, fibroblasts and endothelial cells (ECs), mixed within optimal alginate/gelatin hydrogels and then bioprinted on a microelectrode plate for drug testing. Bioprinted CSs maintained their structure and viability for at least 30 d after printing. Vascular endothelial growth factor (VEGF) promoted EC branching from CSs within hydrogels. Alginate/gelatin-based hydrogels enabled spheroids fusion, which was further facilitated by addition of VEGF. Bioprinted CSs contracted spontaneously and under stimulation, allowing to record contractile and electrical signals on the microelectrode plates for industrial applications. Taken together, our findings indicate that bioprinted CSs can be used to biofabricate human heart tissues for long term in vitro testing. This has the potential to be used to study biochemical, physiological and pharmacological features of human heart tissue.

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Photocrosslinked Silk Fibroin Microgel Scaffolds for Biomedical Applications

Karimi,

Farbehi,

Ziaee

et al. 2024

Adv Funct Materials

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Silk fibroin hydrogels are extensively explored for tissue engineering and regenerative medicine as an artificial extracellular matrix (ECM) that can support tissue growth. However, the nanometer pore size of hydrogels limits adequate cell, tissue, and vascular infiltration. Microgel scaffolds are an emerging class of microporous biomaterials formed by annealing small microscale hydrogels (microgels) into a 3D construct. In this work, silk microgels are generated using a microfluidic device that allows tuning of the microgel diameter (100–400 µm) and are stabilized via visible light‐initiated photo‐crosslinking of native tyrosine residues in silk. Microgels are then covalently annealed using silk solution as glue and the same cytocompatible visible light‐initiated crosslinking to form microgel scaffolds. Unlike the nano‐porosity of bulk photo‐crosslinked silk hydrogels, the microgel scaffolds have an average pore diameter of 29 ± 17 or 192 ± 81 µm depending on the microgel size, with enhanced mechanical properties compared to bulk hydrogels. This microporosity supports enhanced cell spreading and proliferation in vitro and increases scaffold remodeling in vivo, encouraging improved tissue infiltration and matrix deposition. The microgel size and material format also affect inflammatory responses in vivo. This work demonstrates that silk microgels and microgel scaffolds are promising candidates for tissue engineering and regenerative medicine applications.

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Silk fibroin photo-lyogels containing microchannels as a biomaterial platform for in situ tissue engineering

Abstract: The biophysical properties of biomaterials are key to directing the biological responses and biomaterial integration and function in in situ tissue engineering approaches. We present silk photo-lyogels, a biomaterial format...

Cited by 18 publications

References 52 publications

Ice Templating Soft Matter: Fundamental Principles and Fabrication Approaches to Tailor Pore Structure and Morphology and Their Biomedical Applications

Ice Templating Soft Matter: Fundamental Principles and Fabrication Approaches to Tailor Pore Structure and Morphology and Their Biomedical Applications

Towards engineering heart tissues from bioprinted cardiac spheroids

Photocrosslinked Silk Fibroin Microgel Scaffolds for Biomedical Applications

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