Silk fibroin regulated mineralization of hydroxyapatite nanocrystals

Kong, Xiangdong; Cui, Fuzhai; Wang, X.M.; Zhang, M.; Zhang, W.

doi:10.1016/j.jcrysgro.2004.06.007

Cited by 161 publications

(109 citation statements)

References 22 publications

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“…In order to clearly reflect conformation transition of the SF in the blend membranes, the spectral ranges of 1800-600 cm -1 were shown in Figure 1B. The amide I and amide II bands for the PASP/SF composite films were shifted from 1658 to 1662 and 1550 to 1542 cm -1 , respectively, and a small sharp peak at 1649 cm -1 appeared, indicating that a partial conformation transition from silk I to silk II structure occurred [11]. After treated with ethanol, the amide I bands were shifted to 1634 cm -1 with a shoulder at 1694 cm -1 , which could be assigned to β-turns [20,21].…”

Section: Results and Discussion 31 Ftirmentioning

confidence: 99%

“…Silk fibroin (SF), used as an appealing alternative polymer, possesses impressive mechanical properties and appreciable bioactivity that make it attractive for artificial bones [9,10]. Kong et al [11] employed SF to regulate the mineralization of HA . The effects of SF content on the microstructure and physico-chemical properties of the composite had also been studied [12].…”

Section: Introductionmentioning

confidence: 99%

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Effect of polyaspartic acid on hydroxyapatite deposition in silk fibroin blend films

L.¹,

Li²,

Ru³

et al. 2010

Express Polym. Lett.

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Abstract. Polyaspartic acid/silk fibroin/hydroxyapatite (PASP/SF-HA) composites have been synthesized by biomimetic processing. SF solution was mixed with different contents of PASP to prepare the PASP/SF blend membranes. After ethanol treatment and premineralization process, the blend membranes were immersed into 1.5 simulated body fluid (1.5 SBF) for 24 h to induce apatite deposition at 37±0.5°C. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) results revealed that a conformation transition of SF occurred after the addition of PASP and ethanol treatment. The FTIR and XRD results also confirmed that the main component of apatite deposition was HA. Scanning electron microscopy (SEM) showed that the content of HA increased with increasing PASP concentration .Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP) results revealed that the Ca/P molar ratio could reach 1.45, which was close to the Ca/P ratio of apatite. It was appropriate to conclude that the increasing content of PASP had a distinct effect on HA deposition in the blend films.

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Section: Results and Discussion 31 Ftirmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of polyaspartic acid on hydroxyapatite deposition in silk fibroin blend films

L.¹,

Li²,

Ru³

et al. 2010

Express Polym. Lett.

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“…In addition, characteristic band at 873 cm -1 noticed in 3D silk matrices only confirmed that the phosphate sites were partially replaced by carbonate confirming the nature of the deposition as carbonated HA ( Figure 8B). Though we used carbonate-free solution in our study [22], the appearance of carbonate peak in apatite is usually an outcome of atmospheric CO 2 . However, the presence of this carbonate-specific band could not be detected in 2D planar films.…”

Section: Atr-ftirmentioning

confidence: 99%

“…Also, the reason as to why mineralisation could not be observed on methanol treated pure fibroin films (without calcium chloride) which contained at least some amount of â-sheet content remained unexplained. Nevertheless, most studies have reported an additional requirement of either pre-exposure of fibroin or its association with calcium-and phosphate-based solutions to promote nucleation and growth of HA nanocrystals [12,13,16,22]. Whether it is the processing parameters during dissolution and reconstitution that disrupt the native structure of the fibroin chain; firstly by dissociating the specific amino acid chains and secondly by hampering their tendency to re-assemble the necessary â-sheet conformation, or if it is the inherent inert nature of fibroin chain that modulates the extent of mineralisation is still under wraps.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of differential mineralisation on native and regenerated silk matrices

Midha¹,

Tripathi²,

Geng

et al. 2016

Materials Science and Engineering: C

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Bone mineralisation is a well-orchestrated procedure triggered by a protein-based template inducing the nucleation of hydroxyapatite (HA) nanocrystals on the matrix. In an attempt to fabricate superior nanocomposites from silk fibroin, textile braided structures made of natively spun fibres of Bombyx mori silkworm were compared against regenerated fibroin (lyophilised and films) underpinning the influence of intrinsic properties of fibroin matrices on HA nucleation. We found that native braids could bind Ca 2+ ions through electrostatic attraction, which initiated the nucleation and deposition of HA, as evidenced by discrete shift in amide peaks via ATR-FTIR. This phenomenon also suggests the involvement of amide linkages in promoting HA nucleation on fibroin. Moreover, CaCl 2 -SBF immersion of native braids resulted in preferential growth of HA along the c-axis, forming needle-like nanocrystals and possessing Ca/P ratio comparable to commercial HA. Though regenerated lyophilised matrix also witnessed prominent peak shift in amide linkages, HA growth was restricted to (211) plane only, albeit at a significantly lower intensity than braids. Regenerated films, on the other hand, provided no crystallographic evidence of HA deposition within 7 days of SBF immersion. The present work sheds light on the primary fibroin structure of B.mori which probably plays a crucial role in regulating template-induced biomineralisation on the matrix. We also found that intrinsic material properties such as surface roughness, geometry, specific surface area, tortuosity and secondary conformation exert influence in modulating the extent of mineralisation. Thus our work generates useful insights and warrants future studies to further investigate the potential of bone mimetic, silk/mineral nanocomposite matrices for orthopaedic applications.

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“…Natural bone is an innate example of inorganic-organic biocomposites, with a composition of approximately 70 wt% inorganic crystals hydroxyapatite (HAP) with a chemical formula of Ca 10 (PO 4 ) 6 (OH) 2 and 30 wt% of organic matrix. 1,2 The organic matrix is mainly type I collagen which provides bone with flexibility and resilience, and the inorganic phase is responsible for the stiffness and strength of bone.…”

Section: Introductionmentioning

confidence: 99%

Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering

Zhou¹,

Yao²,

Wang³

et al. 2015

IJN

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In bone tissue engineering, collagen/hydroxyapatite (HAP) fibrous composite obtained via electrospinning method has been demonstrated to support the cells’ adhesion and bone regeneration. However, electrospinning of natural collagen often requires the use of cytotoxic organic solvents, and the HAP crystals were usually aggregated and randomly distributed within a fibrous matrix of collagen, limiting their clinical potential. Here, an effective and greener method for the preparation of collagen/HAP composite fibers was developed for the first time, and this green product not only had 40 times higher mechanical properties than that previously reported, but also had an excellent microstructure similar to that of natural bone. By dissolving type I collagen in environmentally friendly phosphate buffered saline/ethanol solution instead of the frequently-used cytotoxic organic solvents, followed with the key step of desalination, co-electrospinning the collagen solution with the HAP sol, generates a collagen/HAP composite with a uniform and continuous fibrous morphology. Interestingly, the nano-HAP needles were found to preferentially orient along the longitudinal direction of the collagen fibers, which mimicked the nanostructure of natural bones. Based on the characterization of the related products, the formation mechanism for this novel phenomenon was proposed. After cross-linking with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/ N -hydroxysuccinimide, the obtained composite exhibited a significant enhancement in mechanical properties. In addition, the biocompatibility of the obtained composite fibers was evaluated by in vitro culture of the human myeloma cells (U2-OS). Taken together, the process outlined herein provides an effective, non-toxic approach for the fabrication of collagen/HAP composite nanofibers that could be good candidates for bone tissue engineering.

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Silk fibroin regulated mineralization of hydroxyapatite nanocrystals

Cited by 161 publications

References 22 publications

Effect of polyaspartic acid on hydroxyapatite deposition in silk fibroin blend films

Effect of polyaspartic acid on hydroxyapatite deposition in silk fibroin blend films

Elucidation of differential mineralisation on native and regenerated silk matrices

Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering

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