Silk fibroin scaffolds with a micro-/nano-fibrous architecture for dermal regeneration

Li, Xiufang; You, Renchuan; Luo, Zuwei; Chen, Guo; Li, Mingzhong

doi:10.1039/c6tb00213g

Cited by 60 publications

(35 citation statements)

References 36 publications

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“…Porous SF materials have potential as scaffolds for dermal tissue regeneration (X. Li et al, ; M. Zhu et al, ). Promoting the vascularization of scaffolds is still the vital challenge in effectively inducing dermal tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…Constructing a scaffold that vascularizes rapidly is the key challenge in dermal tissue regeneration (Hegen et al, ; Xiong et al, ). Three‐dimensional porous scaffolds were designed and modified to promote vascularization by various methods, such as modification of the physical structure (X. Li, You, Luo, Chen, & Li, ), coculturing with vessel‐forming cells (Edwards et al, ; Eke, Mangir, Hasirci, MacNeil, & Hasirci, ), and the incorporation of growth factors (Chiu, Weisel, Li, & Radisic, ; Qu et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, in contrast to biodegradable natural materials such as collagen and gelatin, SF purification does not require cell fragmentation, which reduces the risk of contamination, and SF therefore shows low antigenicity (Kundu et al, ; Tallawi et al, ). Three‐dimensional porous SF scaffolds can be used as a temporary artificial extracellular matrix to provide space for cell proliferation, differentiation, and migration (Zhang, Yan, & Li, ; M. Zhu et al, ), and they exhibit potential for dermal tissue regeneration (X. Li et al, ; Yan et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Wang

Jiang

et al. 2019

J Tissue Eng Regen Med

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Vascularization remains a critical challenge in dermal tissue regeneration. In this study, a vascular endothelial growth factor (VEGF165) and angiopoietin‐1 (Ang‐1) dual gene coexpression vector that encoded green fluorescent protein (GFP) was constructed from an arginine–glycine–aspartic acid‐modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze‐drying method. In vitro, the human endothelial‐derived cell line EA.hy926 was infected with adenovirus vectors and then expressed GFP, secreted VEGF165 and Ang‐1, and promoted cell proliferation effectively. The VEGF165 and Ang‐1 genes loaded in the SF scaffolds significantly promoted the formation of abundant microvascular networks in the chick embryo chorioallantoic membrane. In vivo, angiogenic genes loaded in the scaffolds promoted vascularization and collagen deposition in scaffolds, thus effectively accelerating dermal tissue regeneration in a dorsal full‐thickness skin defect wound model in Sprague–Dawley rats. In conclusion, SF scaffolds loaded with arginine–glycine–aspartic acid‐modified adenovirus vectors encoding VEGF165 and Ang‐1 could stimulate the formation of vascular networks through the effective expression of target genes in vascular endothelial cells, thereby accelerating the regeneration of dermal tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Wang

Jiang

et al. 2019

J Tissue Eng Regen Med

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“…Cells can respond to the surrounding environment and show different behaviors. Micro- and nanostructures of biomaterials have shown great importance in guiding cell migration, as well as in influencing cell adhesion, proliferation, and differentiation [96,97]. …”

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

404

241

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The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomaterials. Silk fibroin (SF), extracted mainly from silkworms, has become a popular biomaterial due to its excellent biocompatibility, exceptional mechanical properties, tunable degradation, ease of processing, and sufficient supply. As a material with excellent processability, SF can be processed into various forms with different structures, including particulate, fiber, film, and three-dimensional (3D) porous scaffolds. This review discusses and summarizes the various constructions of SF-based materials, from single structures to multi-level structures, and their applications. In combination with single structures, new techniques for creating special multi-level structures of SF-based materials, such as micropatterning and 3D-printing, are also briefly addressed.

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“…Silk fibroin (SF) is a natural protein produced by the domestic Bombyx mori or wild silkworm. SF holds great potential for tissue engineering because of its excellent biocompatibility and biodegradability . Unfortunately, although natural silk fibers possesses excellent mechanical properties, regenerated SF fibers are known to have poor mechanical properties, mainly due to the degradation of fibroin molecule and the destruction of crystal structure after regeneration processing .…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of electrospun PCL/Antheraea pernyisilk fibroin nanofibrous scaffolds

Zhang

et al. 2016

Polym Eng Sci

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To engineer tissue restoration, it is necessary to provide a bioactive, mechanically robust scaffold. Electrospun poly(ε‐caprolactone) (PCL) nanofiber is a promising biomaterial candidate with excellent mechanical properties, but PCL scaffolds are inert and lack natural cell recognition sites. To overcome this problem we investigated the incorporation of Antheraea pernyi silk fibroin (ASF) containing inherent RGD tripeptides with PCL in electrospinning process. The mixing ratios showed remarkable impact on the properties of hybrid nanofibers. Increasing PCL content significantly enhanced the mechanical properties of nanofibers. In particular, the mechanical properties were remarkably enhanced when PCL content increased from 50 wt% to 70 wt%. Moreover, the biological assays based on endothelial cells showed promoted cell viability when ASF content reached to 30 wt%. The data demonstrated that the nanofiber containing 70% of PCL and 30% of ASF achieved the most balanced performances for integrating the mechanical properties of PCL and the bioactivity of ASF. Furthermore, biomimetic alignment of 70PCL/30ASF nanofibers was achieved, which could support PC12 neuron‐like cell growth and guide neurite outgrowth, providing a potentially useful option for the engineering of oriented tissues. The results show that the PCL/ASF hybrid nanofibers can be considered as a promising candidate for tissue engineering scaffolds. POLYM. ENG. SCI., 57:206–213, 2017. © 2016 Society of Plastics Engineers

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Silk fibroin scaffolds with a micro-/nano-fibrous architecture for dermal regeneration

Abstract: A novel silk fibroin scaffold containing micro-/nano-fibers promoted dermal regeneration by providing 3D topographic cues.

Cited by 60 publications

References 36 publications

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Fabrication and characterization of electrospun PCL/Antheraea pernyisilk fibroin nanofibrous scaffolds

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