Silk Fibroin/Gelatin Blend Films Crosslinked with Enzymes for Biomedical Applications

Abstract

Microbial transglutaminase (mTG) and mushroom tyrosinase (MT) are used to crosslink B. mori silk fibroin/gelatin (SF/G) films. Crosslinked and uncrosslinked SF/G films show no phase separation. The thermal behavior and the conformational structure of SF/G films are strongly affected by blending and enzymatic treatment. Formation of high thermally stable crosslinked macromolecular species is observed, suggesting the occurrence of strong intermolecular interactions between the two polymers as confirmed by FT-Raman spectroscopy. Preliminary in vitro tests show that MT-crosslinked blends with G amounts ≥40% and mTG-crosslinked SF/G 60/40 films support C2C12 cardiomyocyte adhesion and proliferation.

Influence of grafting with acrylate compounds on the conformational rearrangements of silk fibroin upon electrospinning and treatment with aqueous methanol

“…The Silk I content in the nanofibres was small, as indicated by the low value of the I 1416 /I 1454 intensity ratio (i.e. 0.47 ± 0.04), which has been used for a semiquantitative evaluation of the Silk I content …”

“…0.47 ± 0.04), which has been used for a semiquantitative evaluation of the Silk I content. [26,44,45] Going from the fabric to the nanofibres, the I 850 /I 830 intensity ratio (sensitive to the average hydrogen-bonding state of Tyr) [44,46,47] increased (Fig. S6, Supporting Information); this trend is consistent with a more unordered conformation of the fibres upon electrospinning and, according to Thomas, [48] indicates that in the nanofibres the Tyr residues experience a hydrophobic environment and are not involved in hydrogen bonds.…”

Construction and Characterization of Three-Dimensional Silk Fibroin-Gelatin Scaffolds

“…It show completely polygonal or triangular cell morphology. These observations confirmed the cell survival and proliferation on SF-G scaffolds, assuring its biocompatibility 25,26) . Levels of cell growth and proliferation on the scaffolds control and scaffolds group were evaluated by MTT and ALP assays in vitro.…”

Enzymatic processing of protein-based fibers

“…In addition, the potential for using tyrosinase to graft the bovine lactoferrin onto Bombyx mori silk fibroin and gelatin was also examined based on the similar mechanism shown in Fig. 9 (Taddei et al 2013;Wang et al 2014). With regard to other polyphenol oxidase for silk modification, Guo et al (2013) investigated the structure and properties of ε-polylysinegrafted silk fibers catalyzed by laccase.…”