Silk gland sericin protein membranes: Fabrication and characterization for potential biotechnological applications

Silk Protein Sericin: Promising Biopolymer for Biological and Biomedical Applications

“…Centrifugation and filtration are carried out to remove all insoluble residues. The solution is then thoroughly dialyzed in distilled water using cellulose tubing (MWCO 6000-8000) for complete removal of urea from solution [22].…”

“…The gland cells are taken away from each part in water with the help of forceps, and the content of the silk gland are put into a beaker with water, which is shaken gently for 30 mins. The supernatant is used as the silk protein sericin solution from the MSG [22].…”

Biopolymeric Nanoparticles

“…These hot water soluble proteins comprise different polypeptides ranging in weight from 24 to 400 kDa [76][77][78][79] and have unusually high serine content (40%) along with significant amounts of glycine (16%) [76,79]. Sericin is comprised of 35% β-sheet and 63% random coil, and has no α-helical content-hence, its partially unfolded state [80][81][82][83]. Sericin nanoparticles, apart from the general advantages of protein nanoparticles, may also offer certain other benefits of the inherent property of sericin.…”

Preparation and Characterization of Keratin Blended Films using Biopolymers for Drug Controlled Release Application

“…Blending with sericin resulted in a tight compact and dark film, while gelatin resulted in a homogeneous texture and yellow pale films. Usually, sericin is composed of different kinds of proteins arranged from 4-350 kDa [18][19] . Therefore, the texture of the blended film should be affected by the various types of keratin and sericin proteins.…”