Silk-Laponite® fibrous membranes for bone tissue engineering

Atrian, Matineh; Kharaziha, Mahshid; Emadi, Rahmatollah; Alihosseini, Farzaneh

doi:10.1016/j.clay.2019.03.038

Cited by 52 publications

(29 citation statements)

References 54 publications

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“…The surface hydrophilicity is one of the significant features for cell culturing substrates determining the protein deposition and cell attachment on them 20 . The surface hydrophilicity of the scaffolds was evaluated by measuring the water contact angle.…”

Section: Resultsmentioning

confidence: 99%

“…Sharifi et al reported PCL/CMC nanofibrous scaffolds exposed more hydrophilic surfaces (51°) comparing to PCL 5 . Atrian et al developed fibrous membranes of silk fibroin (SF)‐ LAP and found that the water contact angle of SF fibers reduced from 60° to 48°, after incorporation of LAP in SF 20 . In the present study, the water contact angle decreased to <10 °, which may be due to the immobilization of hydrophilic LAP and CMC at the nanofiber surface.…”

Section: Resultsmentioning

confidence: 99%

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Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

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The side effects and high cost of growth factors and drugs in bone tissue engineering have led to outstanding investigations of alternative osteoinductive supplements. Herein, the electrospun polycaprolactone (PCL) nanofibers were immobilized with carboxymethyl chitosan (CMC) and laponite nanoplatelets (LAP, 0.5‐3.5 wt%) to fabricate osteoinductive scaffolds for bone tissue engineering. The Fourier‐transform infrared)FTIR(and energy dispersive X‐ray (EDX) spectroscopy confirmed the chemical immobilization of CMC and LAP on the surface of PCL nanofibers. Water contact angle measurements exhibited significant improvement of surface hydrophilicity for immobilized scaffolds (<10°) comparing to the virgin PCL nanofibers (125°). The immobilization of CMC and LAP enhanced the attachment, proliferation, and osteodifferentiation of the seeded human bone marrow mesenchymal stem cells (hBM‐MSCs), as evidenced by increased calcium deposition, alkaline phosphatase (ALP) activity, and Osteonectin gene expression. Therefore, the fabricated scaffolds can serve as an appropriate substrate to support the proliferation and differentiation of stem cells to osteoplasts without any external osteoinductive stimulation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

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“…Conforming to the previous studies about the degumming treatment methods and extraction of silk fibroin 45 , 52 , 53 , first, the silkworm cocoons were cut in to small pieces. Afterwards, in a determined aqueous solution of Na 2 CO 3 (0.21% w/v), the cocoon pieces were boiled during 2 h in order to remove the glue like sericin proteins from the cocoon structure.…”

Section: Methodsmentioning

confidence: 99%

Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

Eivazzadeh‐Keihan

Radinekiyan

Aliabadi

et al. 2021

Sci Rep

125

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Herein, a novel nanobiocomposite scaffold based on modifying synthesized cross-linked terephthaloyl thiourea-chitosan hydrogel (CTT-CS hydrogel) substrate using the extracted silk fibroin (SF) biopolymer and prepared Mg(OH)2 nanoparticles was designed and synthesized. The biological capacity of this nanobiocomposite scaffold was evaluated by cell viability method, red blood cells hemolytic and anti-biofilm assays. According to the obtained results from 3 and 7 days, the cell viability of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold was accompanied by a considerable increment from 62.5 to 89.6% respectively. Furthermore, its low hemolytic effect (4.5%), and as well, the high anti-biofilm activity and prevention of the P. aeruginosa biofilm formation confirmed its promising hemocompatibility and antibacterial activity. Apart from the cell viability, blood biocompatibility, and antibacterial activity of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold, its structural features were characterized using spectral and analytical techniques (FT-IR, EDX, FE-SEM and TG). As well as, given the mechanical tests, it was indicated that the addition of SF and Mg(OH)2 nanoparticles to the CTT-CS hydrogel could improve its compressive strength from 65.42 to 649.56 kPa.

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“…Robust osteoblastic differentiation of hMSCs on attapulgite nanorod/poly(lactic‐ co ‐glycolic acid) electrospun nanocomposite scaffolds without any growth factors like dexamethasone was reported by Wang et al [ 74b ] Montmorillonite [ 9b ] and laponite [ 120 ] (up to 10 wt%) were also utilized to improve the mechanical strength, hydrophilicity, cell viability, mineralization and biodegradation ability of electrospun silk fibrous membranes of silkworm silk ( B. mori ) for bone tissue engineering applications. [ 9b,120 ] Poly(glycerol‐ co ‐sebacate)/laponite scaffolds were prepared by salt leaching for improving ALP activity, mechanically durability, osteogenesis, in vivo biocompatibility as well as biodegradability and mineral deposition for potential bone repair applications. [ 115c ] Interestingly, fibrous structures in combination with high aspect ratio nanoclays such as halloysite and sepiolite have been used more regularly for the preparation of scaffolds or membranes.…”

Section: Biomedical Applications Of Copolymer/clay Nanocompositesmentioning

confidence: 99%

Copolymer/Clay Nanocomposites for Biomedical Applications

Murugesan

Scheibel

2020

Adv Funct Materials

154

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Nanoclays still hold a great strength in biomedical nanotechnology applications due to their exceptional properties despite the development of several new nanostructured materials. This article reviews the recent advances in copolymer/clay nanocomposites with a focus on health care applications. In general, the structure of clay comprises aluminosilicate layers separated by a few nanometers. Recently, nanoclay-incorporated copolymers have attracted the interest of both researchers and industry due to their phenomenal properties such as barrier function, stiffness, thermal/flame resistance, superhydrophobicity, biocompatibility, stimuli responsiveness, sustained drug release, resistance to hydrolysis, outstanding dynamic mechanical properties including resilience and low temperature flexibility, excellent hydrolytic stability, and antimicrobial properties. Surface modification of nanoclays provides additional properties due to improved adhesion between the polymer matrix and the nanoclay, high surface free energy, a high degree of intercalation, or exfoliated morphology. The architecture of the copolymer/clay nanocomposites has great impact on biomedical applications, too, by providing various cues especially in drug delivery systems and regenerative medicine.

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Silk-Laponite® fibrous membranes for bone tissue engineering

Cited by 52 publications

References 54 publications

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

Copolymer/Clay Nanocomposites for Biomedical Applications

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