Silkworm disease diagnosis through molecular approach and their management

Bebitha, B.; Mohanraj, Prashanth; Manimegalai, S.; Mahalingam, C. A.

doi:10.15740/has/ijpp/9.1/343-352

Cited by 5 publications

(2 citation statements)

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“…Pebrine disease is one of the most important known diseases in silkworms, which has caused a decrease in silk production (1). Therefore, the development of more reliable diagnostic tools to identify the causative agent of pebrine disease has always been one of the most important and necessary strategies to combat this disease (14). Several methods have been proposed and used in practice for the diagnosis, among which, PCRbased methods are more accurate.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Pebrine Disease in Silkworm Using Molecular Methods

2022

ARI

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Since pebrine disease, as the most important and dangerous disease in silkworms, spreads horizontally through the spores and vertically through the eggs, combating the disease and eliminating it completely from livestock production has been associated with numerous problems. This project aimed to identify the molecular cause of pebrine disease in silkworms using a sensitive, specific, and accurate method. To this purpose, a 136 bp fragment was selected based on the Nosema bombycis partial SSU rDNA sequence, and a pair of primers was designed. Afterward, using the conventional polymerase chain reaction (PCR) method, the target fragment was amplified and sequenced. After that, to determine the detection sensitivity, using the Real-Time PCR method, 5-fold serial dilutions of N. bombycis DNA were prepared, and the last dilution that produced a fluorescent signal was considered the minimum detection limit. All tests were performed in duplicates. Based on the results of the sensitivity test, the standard curve including Ct values and DNA concentration was used for analysis. Moreover, 80 unknown samples examined by light microscope were evaluated using conventional PCR and Real-Time PCR. Both PCR results showed no amplification for the negative control samples. The findings demonstrated that the lowest detection limit for N. bombycis was less than 6 pg of DNA, while, this amount was 8 ng for conventional PCR. Out of 80 samples examined, 55, 60, and 62 samples were positive for light microscope, conventional PCR, and Real-Time PCR methods, respectively. The findings suggested that the Real-Time PCR method had a higher ability to detect the causative agent of pebrine disease than the conventional PCR method, and both methods were superior to light microscopy. Therefore, due to the fewer steps and higher accuracy of Real-Time PCR, it can be introduced as a suitable method for diagnosing pebrine disease.

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Section: Discussionmentioning

confidence: 99%

Diagnosis of Pebrine Disease in Silkworm Using Molecular Methods

2022

ARI

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“…Pada tingkat petani bermanfaat untuk mengetahui perlakuan apa kurang diperhatikan oleh petani di saat pemeliharaan berlangsung. Menurut Bebitha et al (2016), Pemeliharaan ulatsutera yang berlangsung secara terus menerus dengan periode yang rapat seharusnya disertai pula tindakan preventif disinfeksi yang intensif.…”

Section: Pola Penyebaran Grasserieunclassified

Tingkat Serangan dan Kehilangan Hasil Akibat Penyakit Grasserie pada Sentra Persuteraan Alam Sulawesi Selatan

Nuraeni¹,

Arif²,

Nl³

2018

Jhm

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Grasserie disease is one of the serious diseases caused by viruses. Its symptoms generally appear in the last instar of silkworm larvae or just before the formation of the cocoon. This study aimed to determine the level of attack, yield losses and dispersal patterns of grasserie at three sericulture center in South Sulawesi, Districs (Wajo, Soppeng and Enrekang). At each location 10 farmers were selected purposively as respondents. Direct observation was conducted to the farmer’s rearing rack (on farmer’s rearing bed) of silkworm using five replications. The results showed that the attack rates of grasserie were categorrised as very slight, ranging from 3.06 to 3.72 kgs of yield losses per each box with a uniform pattern of disease spread. Reared of silkworms in accordance with standard operating procedures can reduce the rate of grasserie disease to avoid losing larger cocoon yields.

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