Silkworm plasmatocytes are more resistant than other hemocyte morphotypes to Bombyx mori nucleopolyhedrovirus infection

Hori, Takuma; Kiuchi, Takashi; Shimada, Toru; Nagata, Michio; Katsuma, Susumu

doi:10.1016/j.jip.2012.09.004

Cited by 11 publications

(8 citation statements)

References 11 publications

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“…Haemolymph immune responses such as phagocytosis, encapsulation, and melanisation are associated with resistance to Bt (Rahman et al, 2004;Dubovskiy et al, 2008), whereas phenoloxidase, FAD glucose dehydrogenase, encapsulation, and melanisation have been associated with resistance to baculoviruses (Shelby & Popham, 2006;McNeil et al, 2010;Hori et al, 2013). Our findings here and previously (Shikano et al, 2010) bring into question whether constitutive levels of some of the commonly measured immune factors in the haemolymph are actually good indicators of resistance to Bt and baculoviruses when comparing the effects of plant species.…”

Section: Discussionmentioning

confidence: 50%

Impact of non‐pathogenic bacteria on insect disease resistance: importance of ecological context

Shikano

Olson

Cory

2015

Ecological Entomology

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1. The aerial surface of plants is a habitat for large and diverse microbial communities; termed the phyllosphere. These microbes are unavoidably consumed by herbivores, and while the entomopathogens are well studied, the impact of non-pathogenic bacteria on herbivore life history is less clear.2. Previous work has suggested that consumption of non-entomopathogenic bacteria induces a costly immune response that might decrease the risk of infection. However, we hypothesised that insect herbivores should be selective in how they respond to commonly encountered non-pathogenic bacteria on their host plants to avoid unnecessary and costly immune responses.3. An ecologically realistic scenario was used in which we fed cabbage looper, Trichoplusia ni Hübner, larvae on cabbage or cucumber leaves treated with the common non-entomopathogenic phyllosphere bacteria, Pseudomonas fluorescens and P. syringae. Their constitutive immunity and resistance to a pathogenic bacterium (Bacillus thuringiensis; Bt) and a baculovirus (T. ni single nucleopolyhedrovirus) were then examined.4. While feeding on bacteria-treated leaves reduced the growth rate and condition of T. ni, there was no effect on immunity (haemolymph antibacterial and phenoloxidase activities and haemocyte numbers). Phyllosphere bacteria weakly affected the resistance of T. ni to Bt but the direction of this effect was concentration dependent; resistance to the virus was unaffected. Host plant had an impact, with cucumber-fed larvae being more susceptible to Bt.5. The lack of evidence for a costly immune response to non-entomopathogenic bacteria suggests that T. ni are probably adapted to consuming common phyllosphere bacteria, and highlights the importance of the evolutionary history of participants in multi-trophic interactions.

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Section: Discussionmentioning

confidence: 50%

Impact of non‐pathogenic bacteria on insect disease resistance: importance of ecological context

Shikano

Olson

Cory

2015

Ecological Entomology

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“…To our knowledge, the current study is the first to report an ovary-derived cell line which is resistant to BmNPV infection. BmNPV has previously shown distinct tissue or cell tropism (i.e., BmNPV replicating poorly in the anterior silkglands, midgut, Malpighian tubule, salivary glands and plasmatocytes) [4], [26], [27]. In this study, expression patterns of several genes were analyzed to identify the ovarian cell lines.…”

Section: Discussionmentioning

confidence: 99%

Differential Susceptibilities to BmNPV Infection of Two Cell Lines Derived from the Same Silkworm Ovarian Tissues

Zhang

et al. 2014

PLoS ONE

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We previously established and characterized two insect cell lines (BmN-SWU1 and BmN-SWU2) from Bombyx mori ovaries. Here, we examined their differential susceptibilities to Bombyx mori nucleopolyhedrovirus (BmNPV) despite having originated from the same tissue source. BmN-SWU1 cells were susceptible and supported high titers of BmNPV replication, while BmN-SWU2 cells were resistant to BmNPV infection. Subcellular localization analysis demonstrated that very few BmNPV particles could be imported into BmN-SWU2 cells. However, initiation of BmNPV DNA replication but not amplification was detected in BmN-SWU2 cells after transfection with vA4prm-VP39-EGFP bacmid DNA. BmNPV transcription assays showed that late and very late but not early viral genes apparently were blocked in BmNSWU2 cells by unknown mechanisms. Further syncytium formation assays demonstrated that the BmNPV envelope fusion protein GP64 could not mediate BmN-SWU2 host cell-cell membrane fusion. Taken together, these results indicate that these two cell lines represent optimal tools for investigating host-virus interactions and insect antiviral mechanisms.

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“…Hori et al, 2013;McNeil et al, 2010;Shelby and Popham, 2006). However, any unmeasured potential transgenerational effects on offspring immunity clearly did not enhance offspring resistance to AcMNPV.…”

Section: Discussionmentioning

confidence: 93%

Baculovirus-challenge and poor nutrition inflict within-generation fitness costs without triggering transgenerational immune priming

Shikano

Hua

Cory

2016

Journal of Invertebrate Pathology

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Silkworm plasmatocytes are more resistant than other hemocyte morphotypes to Bombyx mori nucleopolyhedrovirus infection

Cited by 11 publications

References 11 publications

Impact of non‐pathogenic bacteria on insect disease resistance: importance of ecological context

Impact of non‐pathogenic bacteria on insect disease resistance: importance of ecological context

Differential Susceptibilities to BmNPV Infection of Two Cell Lines Derived from the Same Silkworm Ovarian Tissues

Baculovirus-challenge and poor nutrition inflict within-generation fitness costs without triggering transgenerational immune priming

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