Silkworm Silk Fiber Bundles as Improved <i>In Vitro</i> Scaffolds for Skeletal Muscle

Clegg, Matthew H.; Harris, Thomas I.; Zhang, Xiaoli; Barney, Jacob T; Jones, Justin A.; Vargis, Elizabeth

doi:10.1021/acsbiomaterials.0c00987

Cited by 3 publications

(1 citation statement)

References 68 publications

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“…The mechanical characteristics and protein secondary structure composition of silk were correlated with myosin gene expression. This increase in contractile protein gene expression implies that proteinaceous, suspended silk substrates may be desirable for in vitro muscle modelling [ 156 ]. Scaffolds with 3D porous architectures may also be utilized as carriers to provide an appropriate milieu for transplanted cells for their survival and proper functioning.…”

Section: Bioinspiration Of Silk For Biomedical and Tissue Engineering...mentioning

confidence: 99%

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

et al. 2023

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Biomaterial research has led to revolutionary healthcare advances. Natural biological macromolecules can impact high-performance, multipurpose materials. This has prompted the quest for affordable healthcare solutions, with a focus on renewable biomaterials with a wide variety of applications and ecologically friendly techniques. Imitating their chemical compositions and hierarchical structures, bioinspired based materials have elevated rapidly over the past few decades. Bio-inspired strategies entail extracting fundamental components and reassembling them into programmable biomaterials. This method may improve its processability and modifiability, allowing it to meet the biological application criteria. Silk is a desirable biosourced raw material due to its high mechanical properties, flexibility, bioactive component sequestration, controlled biodegradability, remarkable biocompatibility, and inexpensiveness. Silk regulates temporo-spatial, biochemical and biophysical reactions. Extracellular biophysical factors regulate cellular destiny dynamically. This review examines the bioinspired structural and functional properties of silk material based scaffolds. We explored silk types, chemical composition, architecture, mechanical properties, topography, and 3D geometry to unlock the body’s innate regenerative potential, keeping in mind the novel biophysical properties of silk in film, fiber, and other potential forms, coupled with facile chemical changes, and its ability to match functional requirements for specific tissues.

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Section: Bioinspiration Of Silk For Biomedical and Tissue Engineering...mentioning

confidence: 99%

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

et al. 2023

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Emerging Materials in Cultivated Meat: Engineering Sustainable Food Solutions – A Review

Wang,

Li,

et al. 2024

Adv Funct Materials

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As the global population grows, the demand for protein‐enriched foods like meats is rising rapidly. Traditional farming practices face challenges including animal welfare, waste management, and carbon emissions, harming the environment. Consequently, meat substitutes have emerged as a promising area of research and as an alternative to traditional livestock‐sourced meats. Cultivated meat, produced through cell culture techniques, is a key area in cellular agriculture and offers a safe and well‐controlled production process for such future foods. The manufacturing of cultivated meat involves several disciplines, including cell culture, media optimization, scaffold design, and advanced manufacturing, along with nutrition, taste and safety topics to meet consumer acceptance and regulatory approvals. Although several review articles have addressed various aspects of cultivated meat, they tend to focus on specific domains rather than a comprehensive analysis of this transformative technology. This review highlights innovative and applied research findings in the field of cultivated meat, with a focus on critical aspects such as nutrition, cells, materials, and scaffold manufacturing technologies. Furthermore, the socio‐political and economic impacts of cultivated meat are explored, and practical recommendations for low‐cost and large‐scale production. Finally, the review also addresses existing challenges and outlines future directions for the development of cultivated meat.

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Bioengineered Silk Protein‐Based 3D In Vitro Models for Tissue Engineering and Drug Development: From Silk Matrix Properties to Biomedical Applications

Shuai,

Zheng,

Kundu

et al. 2024

Adv Healthcare Materials

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Abstract3D in vitro model has emerged as a valuable tool for studying tissue development, drug screening, and disease modeling. 3D systems can accurately replicate tissue microstructures and physiological features, mirroring the in vivo microenvironment departing from conventional 2D cell cultures. Various 3D in vitro models utilizing biomacromolecules like collagen and synthetic polymers have been developed to meet diverse research needs and address the complex challenges of contemporary research. Silk proteins, bearing structural and functional similarities to collagen, have been increasingly employed to construct advanced 3D in vitro systems, surpassing the limitations of 2D cultures. This review examines silk proteins' composition, structure, properties, and functions, elucidating their role in 3D in vitro models. Furthermore, recent advances in biomedical applications involving silk‐based organoid models are discussed. In particular, the unique physiological attributes of silk matrix constituents in in vitro tissue constructs are highlighted, providing a meticulous evaluation of their importance. Additionally, it outlines the current research hurdles and complexities while contemplating future avenues, thereby paving the way for developing complex and biomimetic silk protein‐based microtissues.

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Silkworm Silk Fiber Bundles as Improved In Vitro Scaffolds for Skeletal Muscle

Cited by 3 publications

References 68 publications

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

Emerging Materials in Cultivated Meat: Engineering Sustainable Food Solutions – A Review

Bioengineered Silk Protein‐Based 3D In Vitro Models for Tissue Engineering and Drug Development: From Silk Matrix Properties to Biomedical Applications

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