Silver<b>nanoparticles biosynthesised</b>using<i>Centella asiatica</i>leaf extract: apoptosis induction in MCF‐7 breast cancer cell line

Fard, Shima Edalat; Tafvizi, Farzaneh; Torbati, Maryam Bikhof

doi:10.1049/iet-nbt.2018.5069

Cited by 40 publications

(14 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cytotoxicity of AgNPs against MCF-7 cells was also reported in several articles. 1 , 41 , 44 , 48 , 51 , 52 , 54 However, the long-term effect of Ag/AgCl-NPs on MCF-7 cell line was not reported. In the present study, the colony of MCF-7 cells was not observed in the synthesized Ag/AgCl-NP-treated wells after two weeks as compared with the non-treated wells.…”

Section: Discussionmentioning

confidence: 99%

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

et al. 2020

View full text Add to dashboard Cite

Recently, green synthesis of silver/silver chloride nanoparticles (Ag/AgCl-NPs) has gained a lot of interest because of the usage of natural resources, rapidness, eco-friendliness, and benignancy. Several researchers reported that silver-based biogenic NPs have both antimicrobial and anticancer properties. In the present study, Ag/AgCl-NPs were synthesized from Zizyphus mauritiana fruit extract, and their antibacterial, antifungal, and antiproliferative mechanisms against human MCF-7 cell lines were evaluated. Synthesis of Ag/AgCl-NPs from the Z. mauritiana fruit extract was confirmed by the changes of color and a peak of the UV–visible spectrum at 428 nm. The nanoparticles were characterized by transmission electron microscopy, energy dispersive X-ray, X-ray powder diffraction, thermal gravimetric analysis, atomic force microscope, and Fourier transform infrared. Antibacterial activity was checked against four pathogenic bacteria and two fungi. Cytotoxicity was checked against human breast cancer cell line (MCF-7) and mice Ehrlich ascites carcinoma (EAC) cells by MTS assay and clonogenicity assay. Cell morphology of the control and nanoparticle-treated MCF-7 cells were checked by Hoechst 33342, YF488-Annexin V, and caspase-3 substrates. The level of reactive oxygen species (ROS) was studied by using 2′,7′-dichlorofluorescein-diacetate staining. Real-time polymerase chain reaction was used for gene expression. Synthesized nanoparticles were heat stable cubic crystals with an average size of 16 nm that contain silver and chlorine with various functional groups. The synthesized Ag/AgCl-NPs inhibited the growth of three pathogenic bacteria ( Bacillus subtilis , Shigella boydii, and Escherichia coli ) and two fungi ( Aspergillus niger and Trichoderma spp.). Ag/AgCl-NPs inhibited the growth of MCF-7 and EAC cells with the IC 50 values of 28 and 84 μg/mL, respectively. No colony was formed in MCF-7 cells in the presence of these nanoparticles as compared with control. Ag/AgCl-NPs induced apoptosis and generated ROS in MCF-7 cells. The expression level of FAS, FADD, and caspase-8 genes increased several folds with the decrease of PARP gene expression. These results demonstrated that the anti-proliferation activity of Ag/AgCl-NPs against MCF-7 cells resulted through ROS generation and induction of apoptosis through the Fas-mediated pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In addition, studies found that asiatic acid improved the developmental ability of early embryos in pigs; the main underlying mechanism was amelioration of oxidative stress and downregulation of the expression of apoptosis-related genes (Qi et al, 2020). The potential therapeutic value of C. asiatica for breast cancer mainly reflected in the promotion of apoptosis of breast cancer cells (Fard et al, 2018).…”

Section: Effects On Gynecological Diseasesmentioning

confidence: 99%

Therapeutic Potential of Centella asiatica and Its Triterpenes: A Review

Sun

et al. 2020

Front. Pharmacol.

186

View full text Add to dashboard Cite

Centella asiatica (also known as Centella asiatica (L.) Urb. or Gotu kola) is a traditional Chinese medicine with extensive medicinal value, which is commonly used in Southeast Asian countries. This study aimed to summarize the effects of C. asiatica and its main components on neurological diseases, endocrine diseases, skin diseases, cardiovascular diseases, gastrointestinal diseases, immune diseases, and gynecological diseases, as well as potential molecular mechanisms, to study the pathological mechanism of these diseases based on the changes at the molecular level. The results showed that C. asiatica and its triterpenoids had extensive beneficial effects on neurological and skin diseases, which were confirmed through clinical studies. They exhibited anti-inflammatory, antioxidative stress, anti-apoptotic effects, and improvement in mitochondrial function. However, further clinical studies are urgently required due to the low level of evidence and lack of patients.

show abstract

“…The total phenol content and avonoid content of C.subradiata aqueous extract and AgNPs green synthesized using the same extract were evaluated by different spectroscopic methods. Different concentrations of the lichen extract (20,40,60, 80 and 100 µg/mL) and AgNPs (2, 4, 6, 8 and 10 µg/mL) were used to perform the analysis. The TPC in the samples were determined using Folin-ciocalteau method [36] and TFC was determined using Aluminium chloride method [37].…”

Section: Determination Of Total Phenol Content (Tpc) and Total Avonoid Content (Tfc)mentioning

confidence: 99%

“…The antioxidant activity of C. subradiata extract and AgNPs were determined using different in-vitro assays. Different concentrations of C. subradiata extract (20,40,60, 80 and 100 µg/mL) and AgNPs (2, 4, 6, 8 and 10 µg/mL) were used. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay was performed by adding 2 ml of DPPH solution in methanol to the aliquots of the samples.…”

Section: In-vitro Antioxidant Activity Of C Subradiata Extract and Agnpsmentioning

confidence: 99%

See 1 more Smart Citation

Bio-Inspired Fabrication of Silver Nanoparticles Using High Altitude Squamulose Lichen Extract and Evaluation of its Antioxidant, Anticandida and Cytotoxic properties

Shanthi¹,

Nallal²,

Anand³

et al. 2021

Preprint

View full text Add to dashboard Cite

Bio-inspired nanoparticle synthesis has attracted substantial interest among the scientific society owing to its eco-friendly and non-toxic nature. In the present study, Silver nanoparticles (AgNPs) were synthesized using high altitude squamulose lichen – Cladonia subradiata and characterized using different techniques. The antioxidant and anticandida activity of AgNPs were evaluated using multiple in-vitro assays. In-silico molecular docking analysis and in-vitro cytotoxic assay was performed to determine the anti-cancer potential of synthesized AgNPs. The results of the spectroscopic studies revealed the successful synthesis of AgNPs and the presence of different functional groups suggesting the involvement of phytocompounds in the reduction and capping of AgNPs. The average size of the AgNPs was 20 nm and predominantly spherical in shape. AgNPs demonstrated excellent DPPH free radicals scavenging activity with an IC50 value of 7.51 ± 0.4 µg/mL. C.albicans was identified as the most susceptible strain from the anticandida studies. Usnic acid and Pulvinic acid exhibited low binding energies and showed excellent inhibition interaction with EGFR lung cancer protein. The in-vitro cytotoxic results were impressive with an IC50 value of 28.75 µg/mL for A549 lung cancer cells treated with AgNPs. Thus, the study demonstrates the effective and non-toxic synthesis of AgNPs using a less explored lichen extract as a promising anticandida and anticancer agent in the field of nano-medicine.

show abstract

Silvernanoparticles biosynthesisedusingCentella asiaticaleaf extract: apoptosis induction in MCF‐7 breast cancer cell line

Cited by 40 publications

References 32 publications

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

Therapeutic Potential of Centella asiatica and Its Triterpenes: A Review

Bio-Inspired Fabrication of Silver Nanoparticles Using High Altitude Squamulose Lichen Extract and Evaluation of its Antioxidant, Anticandida and Cytotoxic properties

Contact Info

Product

Resources

About