Silver‐doped 58S bioactive glass as an anti‐Leishmania agent

Abstract

Bioactive glasses (BG) incorporating antimicrobial agents can be effectively used against microorganisms. In this work, the in vitro effectiveness of silver-doped 58S BG (BGAg) against Leishmania species was studied. BG, BGAg1, and BGAg2 belonging to the system 58SiO 2 •(36-x) CaOÁ6P 2 O 5 ÁxAg 2 O, where x=0, 1, and 2 mol.% Ag, were synthesized via sol-gel, and characterized by scanning electron (SEM) and atomic force (AFM) microscopy, thermogravimetric analyses (TGA), X-ray diffraction (XRD), Fourier-transform infrared (FTIR), and surfaceenhanced Raman (Raman-SERS) spectroscopy. Cytotoxicity was assessed in A549 lung adenocarcinoma cells. Leishmania amazonensis and Leishmania braziliensis cultures were exposed to all groups, and C57BL/6 macrophages were infected by over metacyclic form L. amazonensis under the exposure of BGAg particles. SEM and AFM images showed an irregular and network arranged surface. TGA, XRD, FTIR, and RAMAN-SERS analyses confirmed silver inclusion within BG. None of the BG and BGAg presented toxicity. BGAg2 was effective in controlling promastigote forms under 150 and 300 lg/mL concentrations of both evaluated species. On macrophage invasion assay, BGAg2 presented reduction in metacyclic forms. For 72 hours, BGAg1 (150 lg/mL), BGAg1 (300 lg/

Potential of Bioactive Glasses for Cardiac and Pulmonary Tissue Engineering

“…Up to now, most of these studies have been conducted on the skin and nerve tissues with satisfactory results [ 102 , 103 , 104 , 105 ]. Furthermore, the use of melt-derived and sol-gel BGs in the repair and regeneration of cardiac and lung tissues has also been reported with early promising results [ 73 , 74 , 75 , 76 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 ]. In this pioneering set of studies, BGs have been usually incorporated in polymeric matrices that match better the physico-mechanical properties of soft and delicate cardiac/lung tissues.…”

“…It was also reported that doping of 58S sol-gel glass with moderate amounts of silver (2 mol % Ag 2 O max.) to obtain an antibacterial BG elicited no toxic effects towards A549 cells in vitro [ 87 ].…”

Osteogenic potential of sol–gel bioactive glasses containing manganese

“…Ions such as silver and gallium have demonstrated antibacterial properties [19,20], whereas cobalt can stimulate new blood vessel formation [21,22], similar effect observed in copper-doped glasses [23]. Those ions can be released during the glass dissolution process, improving the glass biological effect.…”

Calcium phosphate submicrometric fibers produced by solution blow spinning

“…Other samples presented cell viability rates of about 70%, with no significant difference between these values. The test depicted in Figure 7 suggests that the material was highly biocompatible 33 , but the mechanism of cell/fiber interaction will be examined in future studies. Figure 8 depicts the in vitro bioactivity of fibers immersed in SBF solution for 7 days and 14 days.…”

“…Due to its nanofibrous structure, the surface of the fibers allows biomimicry to accommodate and induce the formation of apatite. These nanofibers act as nuclei for the crystallization process 33 . This is confirmed by high surface area nanometric and submicrometric fibers used in place of commonly agglomerated powders 12,[35][36][37][38] .…”