Silver-gold-apoferritin nanozyme for suppressing oxidative stress during cryopreservation

Dashtestani, Fariba; Ghourchian, Hedayatollah; Najafi, Ali

doi:10.1016/j.msec.2018.10.008

Cited by 40 publications

(23 citation statements)

References 61 publications

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“…The former includes superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx) and catalase (CAT), while the latter includes reduced glutathione (GSH), vitamins, plant extracts (e.g., cinnamtannin B-1), minerals, amino acids, proteins and other exogenous compounds (e.g., resveratrol or quercetin) with antioxidant properties [123,139]. Recent studies investigated the addition of different nanoparticles to the freezing medium to overcome the main drawbacks that conventional antioxidants could present, like the low durability to harsh conditions [140][141][142][143]. Nanotechnology advances have contributed to the design of novel nano-compounds that possess antioxidant properties, such as selenium, zinc oxide and apoferritin containing gold-silver nanoparticles.…”

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

“…Such properties improved viability, membrane integrity and motility of cryopreserved sperm while decreasing apoptosis. Another nanoparticle recently designed to mimic SOD, CAT and GPx activity consisted in a silver-gold nanohybrid in apoferritin cage [143]. Enrichment of semen extender with apoferritin containing gold-silver nanoparticles resulted in a greater sperm viability and motility after thawing due to the reduction of ROS levels and apoptosis during cryopreservation.…”

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

See 1 more Smart Citation

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

147

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

147

118

View full text Add to dashboard Cite

show abstract

“…Interestingly, AuNPs could also be used for ROS protective effects [162][163][164][165]. It might seem contradictory to what has just been explained, but as mentioned before, the nanozyme activity of AuNPs can be tuned or modulated depending on the surface chemistry of the nanoparticle or the microenvironment in which they are being used.…”

Section: Cell Protectionmentioning

confidence: 99%

Gold Nanozymes: From Concept to Biomedical Applications

et al. 2020

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In recent years, gold nanoparticles have demonstrated excellent enzyme-mimicking activities which resemble those of peroxidase, oxidase, catalase, superoxide dismutase or reductase. This, merged with their ease of synthesis, tunability, biocompatibility and low cost, makes them excellent candidates when compared with biological enzymes for applications in biomedicine or biochemical analyses. Herein, over 200 research papers have been systematically reviewed to present the recent progress on the fundamentals of gold nanozymes and their potential applications. The review reveals that the morphology and surface chemistry of the nanoparticles play an important role in their catalytic properties, as well as external parameters such as pH or temperature. Yet, real applications often require specific biorecognition elements to be immobilized onto the nanozymes, leading to unexpected positive or negative effects on their activity. Thus, rational design of efficient nanozymes remains a challenge of paramount importance. Different implementation paths have already been explored, including the application of peroxidase-like nanozymes for the development of clinical diagnostics or the regulation of oxidative stress within cells via their catalase and superoxide dismutase activities. The review also indicates that it is essential to understand how external parameters may boost or inhibit each of these activities, as more than one of them could coexist. Likewise, further toxicity studies are required to ensure the applicability of gold nanozymes in vivo. Current challenges and future prospects of gold nanozymes are discussed in this review, whose significance can be anticipated in a diverse range of fields beyond biomedicine, such as food safety, environmental analyses or the chemical industry.

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“…The loss of sperm membrane integrity is also tightly correlated with reduced sperm motility [83]. Indeed, the compromise of both of these parameters has been well documented in the context of cryopreservation [84][85][86][87] in which post-thaw sperm samples typically experience a burst of ROS and high levels of OS [85,86,88]. Sperm motility is also highly sensitive to pH and ion concentration (reviewed in [89]).…”

Section: Compromised Sperm Membrane Integritymentioning

confidence: 99%

Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility

2020

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A state of oxidative stress (OS) and the presence of reactive oxygen species (ROS) in the male reproductive tract are strongly correlated with infertility. While physiological levels of ROS are necessary for normal sperm functioning, elevated ROS production can overwhelm the cell’s limited antioxidant defenses leading to dysfunction and loss of fertilizing potential. Among the deleterious pleiotropic impacts arising from OS, sperm motility appears to be particularly vulnerable. Here, we present a mechanistic account for how OS contributes to altered sperm motility profiles. In our model, it is suggested that the abundant polyunsaturated fatty acids (PUFAs) residing in the sperm membrane serve to sensitize the male germ cell to ROS attack by virtue of their ability to act as substrates for lipid peroxidation (LPO) cascades. Upon initiation, LPO leads to dramatic remodeling of the composition and biophysical properties of sperm membranes and, in the case of the mitochondria, this manifests in a dissipation of membrane potential, electron leakage, increased ROS production and reduced capacity for energy production. This situation is exacerbated by the production of cytotoxic LPO byproducts such as 4-hydroxynonenal, which dysregulate molecules associated with sperm bioenergetic pathways as well as the structural and signaling components of the motility apparatus. The impact of ROS also extends to lesions in the paternal genome, as is commonly seen in the defective spermatozoa of asthenozoospermic males. Concluding, the presence of OS in the male reproductive tract is strongly and positively correlated with reduced sperm motility and fertilizing potential, thus providing a rational target for the development of new therapeutic interventions.

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Silver-gold-apoferritin nanozyme for suppressing oxidative stress during cryopreservation

Cited by 40 publications

References 61 publications

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Gold Nanozymes: From Concept to Biomedical Applications

Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility

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