Silver nanoparticle based coatings enhance adipogenesis compared to osteogenesis in human mesenchymal stem cells through oxidative stress

Abstract

Silver nanoparticle based coatings preserve the osteogenesis capacity while promoting the adipogenesis of human mesenchymal stem cells through oxidative stress.

Effects of silica–gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells

“…Immunofluorescent staining was conducted according to a previous report 25 to evaluate the expression of osteogenic marker proteins, including COLI, OPN (on day 7 after induction), and OCN (on day 14 after induction). Briefly, the cells were washed twice with PBS, fixed with 4% (w/v) paraformaldehyde for 30 min, and then permeabilized with 0.2% Triton X-100 for 5 min.…”

Silver nanoparticle modified surfaces induce differentiation of mouse kidney-derived stem cells

“…In a pioneering study we demonstrated the mammalian cells can tolerate greater amount of silver than bacteria. Our nding was also conrmed by others and our follow up studies 3,6,12 which showed that amounts of silver lethal to bacteria did not signicantly affect primary human broblasts and mesenchymal stem cells (MSCs). However, mammalian cells may have different tolerance to silver and our current study demonstrate that, although capable of recovery, the viability of mKSCs was reduced in the early stages of growth.…”

“…The reduced initial viability and attachment of the cell on the silver containing samples could be due to the substantial amount of released silver ions from the surfaces. 6,12 The silver ions are the active species that make silver such a potent antibacterial agent but the element could be toxic to mammalian cells too. In a pioneering study we demonstrated the mammalian cells can tolerate greater amount of silver than bacteria.…”

“…We have recently looked at the effect of silver on the differentiation of mesenchymal stem/stromal cell (MSC) and found that oxidative stress caused by the silver led to preferential differentiation to adipocytes rather than osteoblasts. 12 This report demonstrated the need for greater attention to how silver may affect stem cells.…”

“…The method of preparation also allowed us to generate two different silver nanoparticle surface concentrations which facilitated examination of dose dependent effects. 6,12 The effect of the silver nanoparticle modied surface on the attachment, proliferation, viability, spreading and differentiation of mKSCs was evaluated via microscopic and immunostaining techniques.…”