Silver nanoparticles: a potential nanocatalyst for the rapid degradation of starch hydrolysis by α-amylase

Ernest, Vinita; Shiny, Punalur John; Mukherjee, Amitava; Chandrasekaran, N.

doi:10.1016/j.carres.2012.02.009

Cited by 60 publications

(31 citation statements)

References 16 publications

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“…Increased activity of glucose oxidase was noticed when immobilized onto AgNPs [7]. AgNPs, a potential nanocatalyst for the rapid degradation of starch hydrolysis by -amylase, were studied by researchers [8]. The functionlised AuNPs have been extensively used for the detection of various enzymes and also for measurement of their activity [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Saware

Aurade

Jayanthi

et al. 2015

Journal of Nanoparticles

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Amylase is one of the important digestive enzymes involved in hydrolysis of starch. In this paper, we describe a novel approach to study the interaction of amylase enzyme with gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) and checked its catalytic function. AuNPs are synthesized using citrate reduction method and AgNPs were synthesized using biological route employing Ficus benghalensis and Ficus religiosa leaf extract as a reducing and stabilizing agent to reduce silver nitrate to silver atoms. A modulatory effect of nanoparticles on amylase activity was observed. Gold nanoparticles are excellent biocompatible surfaces for the immobilization of enzymes. Immobilized amylase showed 1-to 2-fold increase of activity compared to free enzyme. The biocatalytic activity of amylase in the bioconjugate was marginally enhanced relative to the free enzyme in solution. The bioconjugate material also showed significantly enhanced pH and temperature stability. The results indicate that the present study paves way for the modulator degradation of starch by the enzyme with AuNPs and biogenic AgNPs, which is a promising application in the medical and food industry.

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Section: Introductionmentioning

confidence: 99%

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Saware

Aurade

Jayanthi

et al. 2015

Journal of Nanoparticles

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“…al. 6 seems to be a simple and cheap way to produce silver nanoparticles. Nanosilver particles are likely to agglomerate, but the solution with starch synthesized nanosilver was stable for one month.…”

Section: Resultsmentioning

confidence: 99%

“…It proves that the presence of AgNPs increases the rate of enzymatic degradation of starch by -amylase. It is considered that -amylase was immobilized onto the surface of nanoparticles 6 .…”

Section: Starch Degradationmentioning

confidence: 99%

Nanosilver: A Catalyst in Enzymatic Hydrolysis of Starch

Falkowska

Molga

2014

Polish Journal of Chemical Technology

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Silver nanoparticles are widely used, because of their antimicrobial properties. In this paper, the rate of starch digestion in the presence of nanocatalyst was compared with the rate of reaction without nanosilver. The rate of enzymatic degradation of starch was found to be increased in the presence of silver nanoparticles. It is considered that α-amylase was immobilized onto the surface of nanoparticles.

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“…Lysozyme and amylase from different sources were functionalized over the AgNP, and the corresponding substrates were added and assessed. An increase in the enzymatic activity of enzymes was reported, and lysozyme along with AgNPs was found to have higher bactericidal properties than the free/unbound enzyme [6].In all these studies recorded using various spectroscopic techniques, the structure of enzymes showed few modifications, whereas the function (enzyme activity) was unchanged while being pronounced in the presence of AgNPs [7]. Thus, bio-functionalization not only prevents aggregation but also helps in the immobilization of nanoparticles thereby improving the overall enzyme efficiency [8].…”

mentioning

confidence: 99%

“…In all these studies recorded using various spectroscopic techniques, the structure of enzymes showed few modifications, whereas the function (enzyme activity) was unchanged while being pronounced in the presence of AgNPs [7]. Thus, bio-functionalization not only prevents aggregation but also helps in the immobilization of nanoparticles thereby improving the overall enzyme efficiency [8].…”

mentioning

confidence: 99%

Bio-Functionalized Nanoparticles – A Boon for Nano-drug Therapy

Ernest¹,

M²,

Nagarajan³

2017

J Nanomed Nanotechnol

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Bio-functionalization of nanoparticles has received enormous attention due to reduced toxicity as well as stability in colloidal state which prevents the nanoparticles from aggregation [1,2]. The biomolecules being used for functionalization vary from simple to complex macromolecules [3]. Starch, a natural polymer widely used in many industries as raw material was chosen for synthesis of silver nanoparticles as well as for functionalization. The one-pot synthesis was an easy procedure for both synthesis and functionalization [4]. The aldehyde group of starch serves as the functional group which attaches to the synthesized nanoparticles, thereby preventing aggregation. This stabilizes the nanoparticles, and no aggregation was visible for more than three months. A study was done to check the function and structure of starch using hydrolysis. The results were quite interesting in that the structure had no major deformities while still maintaining functionality [5].Since the substrate starch is being fixed onto the surface of silver nanoparticles (AgNPs), the enzyme-substrate complex reaction was proceeding faster than expected. The blue-black colour disappeared 1.5X faster in the presence of AgNPs. The AgNPs were also functionalized with other enzymes to assess structural and functional properties. Lysozyme and amylase from different sources were functionalized over the AgNP, and the corresponding substrates were added and assessed. An increase in the enzymatic activity of enzymes was reported, and lysozyme along with AgNPs was found to have higher bactericidal properties than the free/unbound enzyme [6].In all these studies recorded using various spectroscopic techniques, the structure of enzymes showed few modifications, whereas the function (enzyme activity) was unchanged while being pronounced in the presence of AgNPs [7]. Thus, bio-functionalization not only prevents aggregation but also helps in the immobilization of nanoparticles thereby improving the overall enzyme efficiency [8].In summary, the immobilization of enzymes over the surface of AgNP has yielded a nano-bio-conjugate with synergistic antibacterial and catalytic properties. This strategy opens the door for the development of potential nano-drug delivery systems wherein the biofunctionalized nanoparticles are an immediate option availed in administration of drugs.

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Silver nanoparticles: a potential nanocatalyst for the rapid degradation of starch hydrolysis by α-amylase

Cited by 60 publications

References 16 publications

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Modulatory Effect of Citrate Reduced Gold and Biosynthesized Silver Nanoparticles on α-Amylase Activity

Nanosilver: A Catalyst in Enzymatic Hydrolysis of Starch

Bio-Functionalized Nanoparticles – A Boon for Nano-drug Therapy

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