Silver Nanoparticles and Mitochondrial Interaction

Bressan, Eriberto; Ferroni, Letizia; Gardin, Chiara; Rigo, Chiarafrancesca; Stocchero, Michele; Vindigni, Vincenzo; Cairns, Warren R. L.; Zavan, Barbara

doi:10.1155/2013/312747

Cited by 94 publications

(55 citation statements)

References 28 publications

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“…100] and calcium sequestration. 101,102 To date, a small number of studies have investigated direct mitochondrial uptake of AgNPs, 45,103,104 and a subset of these studies have found evidence of AgNP entry into the mitochondrion. 45,103 The magnitude of AgNP exposure resulting in mitochondria-specific dysfunction (such as decreased ATP production, observed in fibroblasts and glioblastoma 103 and oral squamous carcinoma cells 81 ) in the cell as a whole varies and is dependent upon factors such as the rate of cellular turnover and age of the organism.…”

Section: Discussionmentioning

confidence: 99%

A systematic review of evidence for silver nanoparticle-induced mitochondrial toxicity

Maurer

Meyer

2016

Environ. Sci.: Nano

106

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Section: Discussionmentioning

confidence: 99%

A systematic review of evidence for silver nanoparticle-induced mitochondrial toxicity

Maurer

Meyer

2016

Environ. Sci.: Nano

106

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“…A recent study on patients with burns showed that fibroblasts deep under burned skin could have nAg uptake released from Acticoat (a nanocrystalline silver dressing), and nAg was located close to the outer membrane of mitochondria. 55 Mitochondrial disruption includes reduction of the membrane potential and subsequent disorders of the oxidationÀreduction system linked to energy metabolism. To this end, we closely looked at alterations of mitochondrial membrane potential.…”

Section: Articlementioning

confidence: 99%

Nanosilver Incurs an Adaptive Shunt of Energy Metabolism Mode to Glycolysis in Tumor and Nontumor Cells

Chen

Wang

et al. 2014

ACS Nano

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anomaterials are currently used in a wide range of products with more prospective applications; however, people raised concerns about their safety profiles. This is because nanomaterials have novel physicochemical properties that can interact with biological systems to generate toxicity. 1 To date, most studies have focused on cytotoxicity of nanomaterials at high concentrations that incur significant injuries to cells in vitro and in animals; however, the high dosage used is often not realistic and fails to consider the potentially detrimental effects on human health under chronic lowdose exposure settings, such as everyday and environmental exposure. 2 Thus, research is urgently needed to study the biological effects at sublethal or even nontoxic concentrations. Nanosilver (nAg), a material that is well-known for its antimicrobial properties, has been extensively used in a wide range of biomedical and consumer products. 3À6 Although many studies have been performed to investigate the cytotoxicity of nAg under different settings, 7À9 relatively little study has been conducted to understand the biological effects of nAg under nontoxic concentrations, which likely precede the toxicological processes or can be differentiated from cytotoxic effects but could perturb cellular homeostasis.Under normal conditions, cells maintain a balanced energy homeostasis through concertedly regulated signaling and metabolic pathways. Namely, there is a perfect equilibrium between anabolism and catabolism

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“…They have been associated with increased adherence to bacterial cell wall, increasing membrane permeability and subsequently bacterial death (17,21). AgNP enhance the release of silver ions, affecting the bacterial respiratory chain because of reactive oxygen species (ROS) and reactive nitrogen species (RNS) production (22,23) which leads to cell damage and finally apoptosis (13). AgNP also affect bacterial DNA and RNA through denaturation and hamper bacterial replication process (24,25).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

Rodríguez

Ramírez-Mora

Montero

et al. 2016

Odovtos - Int J Dent Sc

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The purpose of this study was to measure the antibacterial efficacy of a dispersion of silver nanoparticles (AgNP) in a citrate medium tested in two E. faecalis strains. AgNP were synthesized, and AgNP citrate medium (AgNP-CM) dispersion was prepared at a concentration of 100 μg/mL. The antibacterial efficacy of AgNP-CM dispersion was evaluated over two E. faecalis strains: ATCC29212 and a wild strain collected from human necrotic teeth. 5% sodium hypochlorite (NaOCl) and sterile saline solution were used as positive and negative controls. 5 and 30-minute contact tests were conducted and each experimental group were replicated 10 times. After 24 hours of incubation, the Log CFU/ mL were calculated. The AgNP obtained showed spherical shapes and had 30-60nm size. 5% NaOCl was able to completely eliminate both E. faecalis strains in all groups, showing a significant statistical difference when compared to AgNP-CM dispersion and negative control groups. AgNP-CM dispersion showed a statistically significant decrease in Log CFU/mL averages (p=0,0006) when compared to the sterile saline solution for the ATCC29212 strain during the 30-minute time. Between the 5-minute and 30-minute groups, a significant bacterial count decrease was also observed (p= 0,0128). The antibacterial efficacy of the dispersion was greater for the ATCC29212 strain than the wild strain, were the effect diminished. AgNP-CM dispersion showed a significantly lower antibacterial efficacy against E. faecalis than the 5% NaOCl at the tested times. RESUMENEl propósito del presente estudio fue medir la eficacia antibacteriana de una dispersión de nanopartículas de plata (AgNP) en medio de citrato ante dos cepas de E. faecalis. Las AgNP fueron sintetizadas y se preparó una dispersión en medio de citrato (AgNP-CM) en una concentración de 100 μg/mL. La eficacia antibacteriana de la dispersión de AgNP-CM fue evaluada ante dos cepas de E. faecalis: la ATCC29212 pura y una cepa silvestre aislada de un tratamiento endodóntico fallido. Se utilizó hipoclorito de sodio al 5% de concentración (NaOCl) y solución salina estéril como controles positivos y negativos. Se realizaron pruebas de contacto durante 5 y 30 minutos y cada grupo experimental se replicó 10 veces. Después de incubar las muestras durante 24 horas, se calcularon las concentraciones Log CFU/mL. Las AgNP obtenidas mostraron formas esféricas y un tamaño entre los 30 y 60nm. El NaOCl 5% logró eliminar por completo las cepas bacterianas en todos los grupos, mostrando una diferencia estadísticamente significativa al compararse con las dispersiones de AgNP-CM y los controles negativos. La dispersión de AgNP-CM mostró una disminución estadísticamente significativa en los promedios Log CFU/mL al compararse con la solución salina estéril para la cepa ATCC29212 a los 30 minutos de contacto. Entre los grupos de 5 y 30 minutos de la dispersión, se observó también una disminución en el conteo bacteriano (p=0,0128). La eficacia antibacteriana de la dispersión fue mayor para la cepa ATCC29212 que para la c...

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Silver Nanoparticles and Mitochondrial Interaction

Cited by 94 publications

References 28 publications

A systematic review of evidence for silver nanoparticle-induced mitochondrial toxicity

A systematic review of evidence for silver nanoparticle-induced mitochondrial toxicity

Nanosilver Incurs an Adaptive Shunt of Energy Metabolism Mode to Glycolysis in Tumor and Nontumor Cells

Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

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