.Silver Nanoparticles Attenuate Inflammation aggravation in Hepatocellular Carcinoma in Rats.

Abstract

Using silver nanoparticles is a promising tactic in healthcare and cancer therapy, either alone or as an adjuvant in traditional chemotherapy. In addition, using gamma radiation in nanoparticles preparation is simple, powerful, and environmentally friendly. The present study aims at assessing the impact of silver nanoparticles (AgNPs) prepared by gamma radiation (γ-radiation) on hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DEN). MTT Cytotoxicity assay of AgNPs has been detected in vitro on Wi38 normal fibroblast and HepG2 hepatocellular carcinoma cell lines. Twenty-eight adult male albino rats were divided into four groups. After HCC development, rats received (10µg/g b. wt.) of AgNPs, three times a week for four weeks. Liver enzymes were investigated. TNF-α, TGF-β, and IL-1β inflammatory markers were detected. Furthermore, the transcriptional factor (NF-κB) was assayed. The obtained results confirmed the selective influence of AgNPs on the HCC cell line in a time and dosedependent manner. Moreover, the role of AgNPs is documented in diminishing the inflammation accompanied by HCC development through decreased inflammatory markers and increased apoptosis.