Silver nanoparticles <i>Clinacanthus Nutans</i> leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines

Yakop, Fahmi Bin; Ghafar, Siti Aisyah Abd; Yong, Yoke Keong; Yazan, Latifah Saiful; Hanafiah, Rohazila Mohamad; Lim, Vuanghao; Eshak, Zolkapli

doi:10.1080/21691401.2018.1452750

Cited by 47 publications

(39 citation statements)

References 29 publications

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“…This indicated that the AgNPs become more potent after reduction of silver ions by the extracts utilized, showing high mortality rate (45%) when applied to HeLa cells ( Figure 7(B,Cviii)). Our findings are in line with the previous studies, suggesting potential use of metallic NPs against cancer cells [38][39][40].…”

Section: Anticancer Activity Using Hela Cell Linesupporting

confidence: 93%

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Zada

Ahmad

Khan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Utilizing novel approaches for the green synthesis of metal nanoparticles are of great importance. Therefore, we reported biogenic synthesis of silver nanoparticles (AgNPs) using extracts of Leptolyngbya strain JSC-1, and their significant applications against pathogenic bacteria and cancerous HeLa cell line. The biofabricated AgNPs were characterized by UV-visible spectroscopy, FTIR, SEM, TEM, DLS and zeta-potential. The as prepared AgNPs were assessed for inhibition of bacterial growth and induction of apoptosis in HeLa cells by different doses of AgNPs was evaluated. UV-visible spectroscopy and FTIR of AgNPs demonstrated the surface plasmon resonance at 413 nm and interaction among extract and nanoparticles, respectively. Electron microscopy revealed the morphology and DLS demonstrated size distribution of the particles (10-100 nm). Zeta potential values were between -47 and 0 mV, indicating stability of the particles. Proliferation of HeLa cells was significantly inhibited and severe cytotoxicity with higher intracellular uptake were observed after applying high concentration of AgNPs. Efficient inhibition zones (17 ± 2 and 21 ± 2 mm) were produced at maximum concentration (100 µl from 1 mg ml stock of AgNPs) for Staphylococcus aureus and Escherichia coli, respectively. These findings reveal that the biofabricated AgNPs possess strong antibacterial activity and ability to induce apoptosis in cancer cell line (HeLa).

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Section: Anticancer Activity Using Hela Cell Linesupporting

confidence: 93%

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Zada

Ahmad

Khan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…Identifying the size and morphology of the plant-mediated syntheses of metallic nanoparticles is important because it will allow for the engineering of nanoparticles with proper shapes and sizes for the maximal accumulation in a malignant tumour. Although nanoparticle size and morphology have significant effects on cytotoxicity potency, other parameters are important as well, such as surface properties, aggregation/agglomeration state, solubility, and surface properties; attached functional groups, surface area, and surface charges also play important roles in influencing the resultant pharmacokinetics and pharmacodynamics of nanoparticles [ 22 , 82 , 83 ].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Plant-Mediated Synthesis of Metallic Nanoparticles: A Systematic Review

Hanan¹,

Chiu

Raoov

et al. 2018

IJMS

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In the field of medicine, nanomaterials, especially those derived using the green method, offer promise as anti-cancer agents and drug carriers. However, the biosafety of metallic nanoparticles used as anti-cancer agents remains a concern. The goal of this systematic review was to compare the cytotoxicity of different plant-mediated syntheses of metallic nanoparticles based on their potency, therapeutic index, and cancer cell type susceptibility in the hopes of identifying the most promising anti-cancer agents. A literature search of electronic databases including Science Direct, PubMed, Springer Link, Google Scholar, and ResearchGate, was conducted to obtain research articles. Keywords such as biosynthesis, plant synthesis, plant-mediated, metallic nanoparticle, cytotoxicity, and anticancer were used in the literature search. All types of research materials that met the inclusion criteria were included in the study regardless of whether the results were positive, negative, or null. The therapeutic index was used as a safety measure for the studied compound of interest. Data from 76 selected articles were extracted and synthesised. Seventy-two studies reported that the cytotoxicity of plant-mediated synthesis of metallic nanoparticles was time and/or dose-dependent. Biosynthesised silver nanoparticles demonstrated higher cytotoxicity potency compared to gold nanoparticles synthesised by the same plants (Plumbago zeylanica, Commelina nudiflora, and Cassia auriculata) irrespective of the cancer cell type tested. This review also identified a correlation between the nanoparticle size and morphology with the potency of cytotoxicity. Cytotoxicity was found to be inversely proportional to nanoparticle size. The plant-mediated syntheses of metallic nanoparticles were predominantly spherical or quasi-spherical, with the median lethal dose of 1–20 µg/mL. Nanoparticles with other shapes (triangular, hexagonal, and rods) were less potent. Metallic nanoparticles synthesised by Abutilon inducum, Butea monosperma, Gossypium hirsutum, Indoneesiella echioides, and Melia azedarach were acceptably safe as anti-cancer agents, as they had a therapeutic index of >2.0 when tested on both cancer cells and normal human cells. Most plant-mediated syntheses of metallic nanoparticles were found to be cytotoxic, although some were non-cytotoxic. The results from this study suggest a focus on a selected list of potential anti-cancer agents for further investigations of their pharmacodynamic/toxicodynamic and pharmacokinetic/toxicokinetic actions with the goal of reducing the Global Burden of Diseases and the second leading cause of mortality.

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“…The cells were centrifuged (Heraeus Megafuge 16 centrifuge, Thermo Scientific) at 1000 rpm for 5 min, and the supernatant was aspirated. Lastly, 5 mL of fresh warm medium was added and pipetted up and down with the pellet, then 50 µL of the cells were added to 50 µL of trypan blue to calculate the cell using hemocytometer (Yakop et al, 2018).…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Biocompatible Nutmeg Oil-Loaded Nanoemulsion as Phyto-Repellent

et al. 2020

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Plant essential oils are widely used in perfumes and insect repellent products. However, due to the high volatility of the constituents in essential oils, their efficacy as a repellent product is less effective than that of synthetic compounds. Using a nanoemulsion as a carrier is one way to overcome this disadvantage of essential oils. Nutmeg oil-loaded nanoemulsion (NT) was prepared using a high speed homogenizer and sonicator with varying amounts of surfactant, glycerol, and distilled water. Using a phase diagram, different formulations were tested for their droplet size and insect repellent activity. The nanoemulsion containing 6.25% surfactant and 91.25% glycerol (NT 6) had the highest percentage of protection (87.81%) in terms of repellent activity among the formulations tested for the 8 h duration of the experiment. The droplet size of NT 6 was 217.4 nm, and its polydispersity index (PDI) was 0.248. The zeta potential value was-44.2 mV, and the viscosity was 2.49 Pa.s at pH 5.6. The in vitro release profile was 71.5%. When the cytotoxicity of NT 6 at 400 µg/mL was tested using the MTS assay, cell viability was 97.38%. Physical appearance and stability of the nanoemulsion improved with the addition of glycerol as a co-solvent. In summary, a nutmeg oil-loaded nanoemulsion was successfully formulated and its controlled release of the essential oil showed mosquito repellent activity, thus eliminating the disadvantages of essential oils.

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Silver nanoparticles Clinacanthus Nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines

Cited by 47 publications

References 29 publications

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Cytotoxicity of Plant-Mediated Synthesis of Metallic Nanoparticles: A Systematic Review

Biocompatible Nutmeg Oil-Loaded Nanoemulsion as Phyto-Repellent

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