Silver nanoparticles induce lipid peroxidation and morphological changes in human lymphocytes surface

Zhornik, E. V.; Baranova, L. A.; Drozd, E. S.; Sudas, Margarita; Châu, Nguyễn Ngọc; Buu, Ngo Quoc; Dung, Trần Thị Ngọc; Чижик, С. А.; Volotovski, I. D.

doi:10.1134/s0006350914030282

Cited by 21 publications

(13 citation statements)

References 20 publications

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“…Similarly, exposure of experimental animals to SNP has been shown to cause anemia, cardiac enlargement, growth retardation and degenerative changes in the liver (Butler et al 2015). With regard to the immune system, the viability of human PBL was decreased in time-and dose-dependent manners upon exposure to SNP in vitro (Ghosh et al 2012;Vergallo et al 2014;Zhornik et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Immunotoxic effects of gold and silver nanoparticles: Inhibition of mitogen-induced proliferative responses and viability of human and murine lymphocytesin vitro

Devanabanda

Latheef

Ramanadham

2016

Journal of Immunotoxicology

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Understanding the effects of nanoparticles (NP) on immune cell functions is essential in designing safe and effective NP-based in vivo drug delivery systems. The immunomodulatory potential of gold nanoparticles (GNP) and silver nanoparticles (SNP) was investigated in vitro using murine splenic and human peripheral blood lymphocytes (PBL) in terms of effects on viability and mitogen-induced proliferation. Hydrodynamic size and number of NP were characterized using NP tracking analysis (NTA); modal diameters of GNP and SNP were 28 (±1.5) and 66 (± 2.7) nm, respectively, with a unimodal distribution. Lymphocytes were incubated with GNP or SNP in the presence/absence of B-or T-cell mitogens and proliferative responses then determined using [3 H]-thymidine incorporation. Concanavalin A (T-cell-specific) and lipopolysaccharide-(B-cell-specific) stimulated responses of murine splenic lymphocytes, as well as phytohemagglutinin (T-cell-specific) and pokeweed mitogen-(B-and T-cell specific) induced responses of human lymphocytes, were significantly inhibited by GNP (25-200 lg/ml) and SNP (12.5-50 lg/ml). However, [3 H]-thymidine incorporation by unstimulated lymphocytes was unaffected in the presence of GNP or SNP. Viability of lymphocytes was determined using trypan blue dye exclusion and was significantly inhibited only at 200 lg GNP/ml and 25 or 50 lg SNP/ml. As mitogen responses are most useful to provide supportive mechanistic information on primary immunotoxicologic functional observations, and so far more comprehensive data (in vivo and in vitro) is still needed, the results nevertheless suggest to us that GNP and SNP might potentially be able to modulate immune responses by impacting on lymphocyte activation. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Immunotoxic effects of gold and silver nanoparticles: Inhibition of mitogen-induced proliferative responses and viability of human and murine lymphocytesin vitro

Devanabanda

Latheef

Ramanadham

2016

Journal of Immunotoxicology

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“…Besides the membrane damage caused by nanoparticles, the status of cell membranes is highly linked to cytoskeleton regulation, because the membrane and cytoskeleton are tightly associated with phosphoinositides [48,49]. Nanoparticle-induced lipid peroxidation reportedly alters cell morphology and membrane roughness in human lymphocytes [50]. Cell aspect ratio and cell spread area were decreased in MNPs@SiO 2 (RITC)-treated cells with round morphology, suggesting that focal adhesion was impaired owing to the imbalance between adhesion and tension caused by MNPs@SiO 2 (RITC) treatment.…”

Section: Discussionmentioning

confidence: 99%

Decrease in Membrane Fluidity and Traction Force Induced by Silica-Coated Magnetic Nanoparticles

Shin

Ketebo

Lee

et al. 2020

Preprint

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Background Nanoparticles are being used increasingly due to their unique physical and chemical properties and small size. It is well-known that nanoparticles cause side effects, however their biophysical assessment remains challenging. We addressed this issue by investigating the effects of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate [MNPs@SiO2(RITC)] on the biophysical aspects, such as membrane fluidity and traction force of human embryonic kidney 293 (HEK293) cells. We further extended our understanding on the biophysical effects of nanoparticles on cells using a combination of metabolic profiling and transcriptomic network analysis. Results Overdose (1.0 μg/µl) treatment of MNPs@SiO2(RITC) induced lipid peroxidation and decreased membrane fluidity in HEK293 cells. During membrane damage, HEK293 cells were morphologically shrunk and aspect ratio of the cells were significantly decreased upon MNPs@SiO2(RITC) treatment. Each of traction force (measured in micropillar) was found to be increased, thereby increasing the total traction force in MNPs@SiO2(RITC)-treated HEK293 cells. Due to the reduction in membrane fluidity and elevation of traction force, velocity of the cell movement was significantly decreased in MNPs@SiO2(RITC)-treated HEK293 cells. Moreover, intracellular ATP also decreased in a dose dependent manner upon MNPs@SiO2(RITC) treatment. To understand the biophysical changes in cells, we analysed transcriptome and metabolic profiles and generated metabotranscriptomics network. The network showed relationships among peroxidation of lipid, focal adhesion, cell movement, and related genes and metabolites. Furthermore, in silico prediction of the network showed increment in the peroxidation of lipid and suppression of focal adhesion and cell movement.Conclusion Taken together, our results demonstrate that overdosage of MNPs@SiO2(RITC) impairs cellular movement, followed by changes in the biophysical properties of cells, thus highlighting the need for biophysical assessment of nanoparticle-induced side effects.

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“…Excessive uses of these particles also increase discharge into aquatic habitats and exist in colloidal form. These particles persuade toxicity to invertebrate or vertebrate cell lines by production of reactive oxygen species (Ali 2014), apoptosis (Piao et al 2011), reduced mitochondrial function (Ahamed et al 2010), lipid peroxidation of membranes (Khan et al 2017b;Zhornik et al 2014), and oxidative stress marker depletion (Arora et al 2009). Furthermore, Ag-NPs generate oxidative stress in target organisms (Devi et al 2015;Khan et al 2017b).…”

Section: Introductionmentioning

confidence: 99%

Ameliorative role of nano-ceria against amine coated Ag-NP induced toxicity in Labeo rohita

2018

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Silver nanoparticles (Ag-NPs) and its byproducts can spread pollution in aquatic habitat. Liver and gills are key target for toxicity. Oxidative stress, tissue alterations, and hemotoxicity are assumed to be associated with Ag-NPs in target animals. Cerium oxide nanoparticles (nano-ceria) show antioxidant potential in scavenging the free radicals generated in Ag-NPinduced oxidative stress. We determined ameliorated role of nano-ceria against Ag-NP-induced toxicity in fresh water Labeo rohita (L. rohita). Four groups were used in study including control, nano-ceria, Ag-NPs, and Ag-NPs + nano-ceria. Ag-NPs (30 mg l −1) and nano-ceria (50 µg kg −1) were given through water and prepared feed, respectively. The samples were taken after 28 days. Results demonstrated that pre-treatment of nano-ceria recovered L. rohita from Ag-NP-induced toxicity and oxidative stress. Nano-ceria pre-treatment actively mimics the activity of GST, GSH, CAT, and SOD. Furthermore, Ag-NPs' treatment caused severe inflammation and necrosis in hepatic parenchyma which leaded to congestion of blood in hepatic tissues. Accumulation of a yellow pigment in hepatic tissue was also seen due to necrosis of affected cells. In nano-ceria pre-treatment, there was no congestion in hepatic tissue. Vacuolization of cells and necrosis in some area was recorded in nano-ceria pre-treated group, but the gill and hepatic tissue showed improvement against Ag-NP-induced damage. Nano-ceria pre-treatment also improved hematological parameters in Ag-NP-treated fish. This study concluded that Ag-NP-induced toxicity in treated fish and pre-treatment of nano-ceria show ameliorative role.

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Silver nanoparticles induce lipid peroxidation and morphological changes in human lymphocytes surface

Cited by 21 publications

References 20 publications

Immunotoxic effects of gold and silver nanoparticles: Inhibition of mitogen-induced proliferative responses and viability of human and murine lymphocytesin vitro

Immunotoxic effects of gold and silver nanoparticles: Inhibition of mitogen-induced proliferative responses and viability of human and murine lymphocytesin vitro

Decrease in Membrane Fluidity and Traction Force Induced by Silica-Coated Magnetic Nanoparticles

Ameliorative role of nano-ceria against amine coated Ag-NP induced toxicity in Labeo rohita

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