Silver nanoparticles inhibit beige fat function and promote adiposity

Yue, Lishu; Zhao, Wenjun; Wang, Dongmei; Meng, Meiyao; Zheng, Ying; Li, Yu; Qiu, Jin; Yu, Jian; Yang, Yan; Lu, Peng; Sun, Youmin; Fu, Jie; Wang, Jiqiu; Zhang, Qiang; Xu, Lingyan; Ma, Xinran

doi:10.1016/j.molmet.2019.01.005

Cited by 28 publications

(21 citation statements)

References 39 publications

(45 reference statements)

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“…The 3T3-L1 cells produce large amounts of ROS during differentiation into adipocytes, which promotes intracellular lipid accumulation and inhibits browning gene expression. , Reducing the excess generation of ROS can effectively inhibit fat accumulation in adipocytes and alleviate obesity-associated metabolic disorders. , MnNPs catalyzed the dismutation of superoxide radicals to H 2 O 2 and the subsequent reduction of H 2 O 2 to water by mimicking SOD and CAT, respectively. In this study, the 3T3-L1 cells were treated with SF@Rsg-Mn, SF@Rsg, SF@Mn, or Rsg-Mn during differentiation.…”

Section: Resultsmentioning

confidence: 99%

Synergistic Treatment of Obesity via Locally Promoting Beige Adipogenesis and Antioxidative Defense in Adipose Tissues

Ding

Chen

Tao

et al. 2021

ACS Biomater. Sci. Eng.

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Obesity is a primary risk factor for type 2 diabetes, cardiovascular diseases, cancer, and other chronic diseases. Current antiobesity medications need frequent administration and show limited efficacy with severe side effects. Herein, browning agent rosiglitazone (Rsg) and antioxidant manganese tetroxide nanoparticles (MnNPs, around 250 nm) are integrated into electrospun short fibers (SF@Rsg-Mn) with a 1.5 μm width and a 20 μm length. Upon injection into inguinal adipose tissues, SF@Rsg-Mn are well retained in the local depots to sustainably release Rsg in 30 days for adipose tissue browning, while MnNPs on the fiber surface continuously scavenge adipose reactive oxygen species (ROS) for an extended period of time. Synergistic inhibition of fat accumulation through ROS scavenging and white adipocyte browning has been demonstrated for the first time, and the optimal synergistic ratio of Rsg and MnNPs is determined to be 1/14 via combination index examination. SF@Rsg-Mn inhibit lipid accumulation through downregulation of adipogenic gene PPARγ while promoting energy expenditure through upregulation of brown-specific gene UCP1 and mitochondrial function gene COX7A1. In a diet-induced obesity mouse model, a single injection of SF@Rsg-Mn into inguinal adipose tissues has accomplished a synergistic effect on body weight loss, fat reduction, glucose, and lipid metabolic improvement while minimizing adverse effects on other tissues, thereby paving the way to efficacious, safe, and practical treatment of obesity.

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Section: Resultsmentioning

confidence: 99%

Synergistic Treatment of Obesity via Locally Promoting Beige Adipogenesis and Antioxidative Defense in Adipose Tissues

Ding

Chen

Tao

et al. 2021

ACS Biomater. Sci. Eng.

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“…At the same time, the authors [ 65 ] showed that big doses of AgNPs can cause adiposis, with the fat tissue gain rate increasing significantly starting from the seventh day after the AgNPs administration. It might be so that the same effect was observed following the administration of the 4–7.5 mg/kg AgNPs-BAC dose ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

In Vivo Study of Entero- and Hepatotoxicity of Silver Nanoparticles Stabilized with Benzyldimethyl-[3-myristoylamine)-propyl]ammonium Chloride (Miramistin) to CBF1 Mice upon Enteral Administration

et al. 2021

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Silver nanoparticles (AgNPs) are the most widely studied antimicrobial nanomaterials. However, their use in biomedicine is currently limited due to the availability of data that prove the nanosilver toxicity associated primarily with oxidative stress development in mammalian cells. The surface modification of AgNPs is a potent technique of improvement of their biocompatibility. The synthetic or natural compounds that combine zero or low toxicity towards human and animal organisms with inherent antimicrobial properties are the most promising stabilizing agents, their use would also minimize the risks of microorganisms developing resistance to silver-based materials. We used a simple technique to obtain 30–60 nm AgNPs stabilized with benzyldimethyl[3-myristoylamine)-propyl]ammonium chloride monohydrate (BAC)—a well-known active ingredient of many antibacterial drugs. The objective of the study was to assess the AgNPs-BAC entero- and hepatotoxicity to CBF1 mice upon enteral administration. The animals were exposed to 0.8–7.5 mg/kg doses of AgNPs-BAC in the acute and to 0.05–2.25 mg/kg doses of AgNPs-BAC in the subacute experiments. No significant entero- and hepatotoxic effects following a single exposure to doses smaller than 4 mg/kg were detected. Repeated exposure to the doses of AgNPs-BAC below 0.45 mg/kg and to the doses of BAC below 0.5 mg/kg upon enteral administration also led to no adverse effects. During the acute experiment, the higher AgNPs-BAC dose resulted in increased quantities of aminotransferases and urea, as well as the albumin-globulin ratio shift, which are indicative of inflammatory processes. Besides, the relative mass of the liver of mice was smaller compared to the control. During the subacute experiment, the groups treated with the 0.25–2.25 mg/kg dose of AgNPs-BAC had a lower weight gain rate compared to the control, while the groups treated with the 2.25 mg/kg dose of AgNPs-BAC showed statistically significant variations in the blood serum transaminases activity, which indicated hepatosis. It should be noted that the spleen and liver of the animals from the groups treated with the 0.45 and 2.25 mg/kg dose of AgNPs-BAC were more than two times smaller compared to the control. In the intestines of some animals from the group treated with the 2.25 mg/kg dose of AgNPs-BAC small areas of hyperemia and enlarged Peyer’s patches were observed. Histological examination confirmed the initial stages of the liver and intestinal wall inflammation.

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“…An exploration of the in vivo and in vitro role of silver nanoparticles in thermogenic beige adipocyte differentiation revealed that AgNP inhibited beige adipocyte differentiation, adipocyte thermogenesis, and mitochondrial functioning. AgNP elevated ROS levels within beige adipocytes and activated the MAPK-ERK signaling pathway which is involved in adipogenesis ( 114 ).…”

Section: Metalsmentioning

confidence: 99%

Obesogens: How They Are Identified and Molecular Mechanisms Underlying Their Action

Mohajer

Checkcinco

et al. 2021

Front. Endocrinol.

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Adult and childhood obesity have reached pandemic level proportions. The idea that caloric excess and insufficient levels of physical activity leads to obesity is a commonly accepted answer for unwanted weight gain. This paradigm offers an inconclusive explanation as the world continually moves towards an unhealthier and heavier existence irrespective of energy balance. Endocrine disrupting chemicals (EDCs) are chemicals that resemble natural hormones and disrupt endocrine function by interfering with the body’s endogenous hormones. A subset of EDCs called obesogens have been found to cause metabolic disruptions such as increased fat storage, in vivo. Obesogens act on the metabolic system through multiple avenues and have been found to affect the homeostasis of a variety of systems such as the gut microbiome and adipose tissue functioning. Obesogenic compounds have been shown to cause metabolic disturbances later in life that can even pass into multiple future generations, post exposure. The rising rates of obesity and related metabolic disease are demanding increasing attention on chemical screening efforts and worldwide preventative strategies to keep the public and future generations safe. This review addresses the most current findings on known obesogens and their effects on the metabolic system, the mechanisms of action through which they act upon, and the screening efforts through which they were identified with. The interplay between obesogens, brown adipose tissue, and the gut microbiome are major topics that will be covered.

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Silver nanoparticles inhibit beige fat function and promote adiposity

Cited by 28 publications

References 39 publications

Synergistic Treatment of Obesity via Locally Promoting Beige Adipogenesis and Antioxidative Defense in Adipose Tissues

Synergistic Treatment of Obesity via Locally Promoting Beige Adipogenesis and Antioxidative Defense in Adipose Tissues

In Vivo Study of Entero- and Hepatotoxicity of Silver Nanoparticles Stabilized with Benzyldimethyl-[3-myristoylamine)-propyl]ammonium Chloride (Miramistin) to CBF1 Mice upon Enteral Administration

Obesogens: How They Are Identified and Molecular Mechanisms Underlying Their Action

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