Silver nanoparticles mitigate Aeromonas hydrophila-induced immune suppression, oxidative stress, and apoptotic and genotoxic effects in Oreochromis niloticus

El-Houseiny, Walaa; Mansour, M.; Mohamed, Wafaa A.M.; Al-Gabri, Naif A.; El‐Sayed, Ahmed A.; Altohamy, Dalia E.; Ibrahim, Rowida E.

doi:10.1016/j.aquaculture.2021.736430

Cited by 43 publications

(23 citation statements)

References 68 publications

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“…The smallest amount of damage was seen in fish treated with the highest AgNPs concentration, 750 µg/L. This likely reflects the health‐promoting and antimicrobial properties of AgNPs, as reported by El‐Houseiny et al (2021).…”

Section: Discussionsupporting

confidence: 66%

“…The lowest levels were seen following treatment with 750 µg/L AgNPs. El‐Houseiny et al (2021) also reported favourable effects on kidney function following AgNPs treatment.…”

Section: Discussionmentioning

confidence: 97%

“…(f) Hepatopancreas of the positive control group (not treated after bacterial challenge) show severe diffuse hydropic change of the hepatocytes in addition to severe congestion and dilatation of hepatic sinusoids and portal veins [Correction added on 2 November 2021, after first online publication: Figure position has been rectified.] to genotoxins and other toxic metabolites produced during bacterial infection(El-Houseiny et al, 2021;Grasso & Frisan, 2015). In contrast, the treatment of fish with AgNPs caused a significant decrease (p < 0.05) in DNA damage in a dose-dependent manner.…”

mentioning

confidence: 98%

“…AgNPs show antibacterial activity via several pathways. Host responses may aid in enhancing these activities (El-Houseiny et al, 2021). The antimicrobial effects of nanoparticles vary depending on the physicochemical characteristics of the particles, the delivery route and the invading pathogen (Lara et al, 2011).…”

mentioning

confidence: 99%

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Antibacterial activity of silver nanoparticles against antibiotic‐resistant Aeromonas veronii infections in Nile tilapia, Oreochromis niloticus (L.), in vitro and in vivo assay

Elgendy

Shaalan

Abdelsalam

et al. 2021

Aquaculture Research

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Antibiotic-resistant Aeromonas veronii isolates (4) were identified from moribund Oreochromis niloticus by phenotypic and genotypic characterization using 16S rDNA gene sequence analysis. Antibacterial efficacy of silver nanoparticles (AgNPs) was assessed in vitro and in vivo against A. veronii isolates. A minimum inhibitory concentration (MIC) and 48 h lethal concentration 50% (LC 50 ) for AgNPs were estimated to be 3.125 μg/ml and 8.77 mg/L respectively. Four hundred fifty fish were divided into 10 groups in triplicate (15/replica). Two control groups were untreated with AgNPs:The first (G1) was not infected, whereas the second (G2) was experimentally infected with A. veronii. AgNPs were added to aquarium water with fish either noninfected (G3-G6) or infected (G7-G10) with A. veronii. AgNPs concentrations were 100, 250, 500 and 750 μg/L, respectively, for both sets of fish groups. Fish infected with A. veronii but untreated with AgNPs showed septicaemic symptoms, a high mortality rate (71%) and poorer haematological parameters (red blood cells [RBCs], haemoglobin concentration [Hb], hematocrit [HCT%], mean cell volume [MCV] and mean cell haemoglobin [MCH]); impaired nonspecific immune responses (total protein, albumin, globulin and lysozymes); reduced antioxidant capacity (catalase [CAT], peroxidase and superoxide dismutase [SOD]); a significant increase in creatinine, uric acid, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP); and genotoxic effects in gills. Treatment with AgNPs increased fish survival; improved haematological, immunological and antioxidant activities; and optimized liver and kidney function. The most favourable outcome was seen using an AgNPs concentration of 750 μg/L. Various histopathological changes were found among experimental fish.

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Section: Discussionsupporting

confidence: 66%

“…The lowest levels were seen following treatment with 750 µg/L AgNPs. El‐Houseiny et al (2021) also reported favourable effects on kidney function following AgNPs treatment.…”

Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 98%

mentioning

confidence: 99%

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Antibacterial activity of silver nanoparticles against antibiotic‐resistant Aeromonas veronii infections in Nile tilapia, Oreochromis niloticus (L.), in vitro and in vivo assay

Elgendy

Shaalan

Abdelsalam

et al. 2021

Aquaculture Research

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“…In order to replace the use of antibiotics in aquaculture, multiple strategies have been actively explored such as vaccines 21,22 , biological control [23][24][25][26] , antimicrobial peptides 27,28 , medicinal herbs extract 29,30 and nanoparticles 31,32 . However, none of these strategies meet the requirement for antibiotic substitution up to date.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Peptide Polymers as Alternatives to Antibiotics Used in Aquaculture

Jiang

et al. 2022

Preprint

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The pressure of antimicrobial resistance has forced many countries to reduce or even prohibit the use of antibiotics in feed. Therefore, it is in urgent need to develop alternatives to antibiotics to control infectious diseases in feed and aquaculture. To address this long-lasting challenge, we prepared peptide polymers that display potent and broad-spectrum activity against common pathogenic bacteria in aquaculture, low hemolysis and low cytotoxicity, and doesn’t induce bacteria to develop resistance or cross resistance to antibiotics. The optimal peptide polymer demonstrates strong in vivo therapeutic potential in an adult zebrafish infection model. Moreover, the optimal peptide polymer is biodegradable by enzyme into single amino acids and dipeptides to totally lose antibacterial activity and, therefore, will not cause antimicrobial selective pressure. Our study suggests that peptide polymers are promising alternatives to antibiotics in aquaculture and open new avenues to address the global challenge of antimicrobial resistance.

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Bio‐reductive synthesis of silver nanoparticles, its antibacterial efficiency, and possible toxicity in common carp fish (Cyprinus carpio)

Kakakhel,

Narwal,

Khan

et al. 2023

Microscopy Res & Technique

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The biological synthesis of nanoparticles is an emerging field of study that seeks to synthesize nanoparticles using non‐chemical mechanisms such as microorganisms, plants, and animal blood serum. Among these, plants have gained particular attention due to their ease of handling, availability, and ability to synthesize a wide range of nanoparticles. Therefore, the current study aimed to fabricate the silver nanoparticles (AgNPs) using Chinese medicinal plants (CMP) for their possible toxicity in common carp fish (Cyprinus carpio). For this purpose, CMP was dried, ground, and used as a bio‐reductive agent. The fabricated AgNPs were characterized and a well dispersed AgNPs were obtained. Moreover, the C. carpio was exposed to the AgNPs for bioaccumulation and histological alterations. The obtained findings revealed that the AgNPs were mostly accumulated in the intestines followed by the gills, muscles, liver, and brain. The accumulated AgNPs caused histological alterations in gills and intestines at the highest concentration (0.08 mg/L). However, very less alterations were caused by the lowest concentration, especially in the intestine. In conclusion, further in‐depth research is needed to determine the risks associated with the usage of nanoparticles to reveal their harmful impacts on fish and the aquatic environment.Highlights The biological fabrication of AgNPs is considered eco‐friendly. Chinese medicinal plants play a significant role in AgNPs synthesis. AgNPs have excellent antibacterial activity. AgNPs are bioaccumulated in various organs of fish.

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Silver nanoparticles mitigate Aeromonas hydrophila-induced immune suppression, oxidative stress, and apoptotic and genotoxic effects in Oreochromis niloticus

Cited by 43 publications

References 68 publications

Antibacterial activity of silver nanoparticles against antibiotic‐resistant Aeromonas veronii infections in Nile tilapia, Oreochromis niloticus (L.), in vitro and in vivo assay

Antibacterial activity of silver nanoparticles against antibiotic‐resistant Aeromonas veronii infections in Nile tilapia, Oreochromis niloticus (L.), in vitro and in vivo assay

Biodegradable Peptide Polymers as Alternatives to Antibiotics Used in Aquaculture

Bio‐reductive synthesis of silver nanoparticles, its antibacterial efficiency, and possible toxicity in common carp fish (Cyprinus carpio)

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