Silver Nanoparticles Promote Neuronal Growth

Alon, Noa; Miroshnikov, Yana; Perkas, Nina; Nissan, Ifat; Gedanken, Aharon; Shefi, Orit

doi:10.1016/j.proeng.2013.05.089

Cited by 18 publications

(11 citation statements)

References 7 publications

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“…If the silica supports were of about 100 µm diameter, the PC 12 cells extended neurites on the Au NPs in presence of an electrical stimulation, whereas if the silica supports were lower in dimensions (20 µm) the neurite outgrowth was enhanced also without electrical stimulation [137]. Moreover, these findings were also confirmed by Alon et al [138] and Nissan et al [139]. In parallel, substrates coated with Ag NPs demonstrated to act as scaffolds to sustain the growth of neuroblastoma cells.…”

Section: Neural Applicationssupporting

confidence: 59%

Inorganic Nanomaterials in Tissue Engineering

et al. 2022

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In recent decades, the demand for replacement of damaged or broken tissues has increased; this poses the attention on problems related to low donor availability. For this reason, researchers focused their attention on the field of tissue engineering, which allows the development of scaffolds able to mimic the tissues’ extracellular matrix. However, tissue replacement and regeneration are complex since scaffolds need to guarantee an adequate hierarchical structured morphology as well as adequate mechanical, chemical, and physical properties to stand the stresses and enhance the new tissue formation. For this purpose, the use of inorganic materials as fillers for the scaffolds has gained great interest in tissue engineering applications, due to their wide range of physicochemical properties as well as their capability to induce biological responses. However, some issues still need to be faced to improve their efficacy. This review focuses on the description of the most effective inorganic nanomaterials (clays, nano-based nanomaterials, metal oxides, metallic nanoparticles) used in tissue engineering and their properties. Particular attention has been devoted to their combination with scaffolds in a wide range of applications. In particular, skin, orthopaedic, and neural tissue engineering have been considered.

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Section: Neural Applicationssupporting

confidence: 59%

Inorganic Nanomaterials in Tissue Engineering

et al. 2022

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“…Therefore, in this study we selected AgNPs as a potential alternative therapeutic agent for OvCSCs. AgNPs have a dual role: at lower concentrations, they can enhance cell survival and differentiation, and at higher concentrations, they can inhibit cell viability in neuronal cells [27]. For instance, AgNPs with an average size of 20 nm and at concentrations up to 2 µg/mL promoted osteogenic differentiation of urine-derived stem cells by inducing actin polymerization and activation of RhoA; whereas AgNO 3 had no such effects [28].…”

Section: Resultsmentioning

confidence: 99%

Differential Cytotoxic Potential of Silver Nanoparticles in Human Ovarian Cancer Cells and Ovarian Cancer Stem Cells

Choi

Park

Han

et al. 2016

IJMS

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The cancer stem cell (CSC) hypothesis postulates that cancer cells are composed of hierarchically-organized subpopulations of cells with distinct phenotypes and tumorigenic capacities. As a result, CSCs have been suggested as a source of disease recurrence. Recently, silver nanoparticles (AgNPs) have been used as antimicrobial, disinfectant, and antitumor agents. However, there is no study reporting the effects of AgNPs on ovarian cancer stem cells (OvCSCs). In this study, we investigated the cytotoxic effects of AgNPs and their mechanism of causing cell death in A2780 (human ovarian cancer cells) and OvCSCs derived from A2780. In order to examine these effects, OvCSCs were isolated and characterized using positive CSC markers including aldehyde dehydrogenase (ALDH) and CD133 by fluorescence-activated cell sorting (FACS). The anticancer properties of the AgNPs were evaluated by assessing cell viability, leakage of lactate dehydrogenase (LDH), reactive oxygen species (ROS), and mitochondrial membrane potential (mt-MP). The inhibitory effect of AgNPs on the growth of ovarian cancer cells and OvCSCs was evaluated using a clonogenic assay. Following 1–2 weeks of incubation with the AgNPs, the numbers of A2780 (bulk cells) and ALDH+/CD133+ colonies were significantly reduced. The expression of apoptotic and anti-apoptotic genes was measured by real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Our observations showed that treatment with AgNPs resulted in severe cytotoxicity in both ovarian cancer cells and OvCSCs. In particular, AgNPs showed significant cytotoxic potential in ALDH+/CD133+ subpopulations of cells compared with other subpopulation of cells and also human ovarian cancer cells (bulk cells). These findings suggest that AgNPs can be utilized in the development of novel nanotherapeutic molecules for the treatment of ovarian cancers by specific targeting of the ALDH+/CD133+ subpopulation of cells.

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“…In the present study, the SH‐SY5Y cells subjected to AgNP exposure for one week showed more significant morphological changes and neurite growth compared to control cells. A recent report also documented that AgNPs immobilized on glass substrates enhanced neurite growth [40].…”

Section: Discussionmentioning

confidence: 99%

Biologically synthesized silver nanoparticles induce neuronal differentiation of SH‐SY5Y cells via modulation of reactive oxygen species, phosphatases, and kinase signaling pathways

Dayem

Kim

Gurunathan

et al. 2014

Biotechnology Journal

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Nano-scale materials are noted for unique properties, distinct from those of their bulk material equivalents. In this study, we prepared spherical silver nanoparticles (AgNPs) with an average size of about 30 nm and tested their potency to induce neuronal differentiation of SH-SY5Y cells. Human neuroblastoma SH-SY5Y cells are considered an ideal in vitro model for studying neurogenesis, as they can be maintained in an undifferentiated state or be induced to differentiate into neuron-like phenotypes in vitro by several differentiation-inducing agents. Treatment of SH-SY5Y cells by biologically synthesized AgNPs led to cell morphological changes and significant increase in neurite length and enhanced the expression of neuronal differentiation markers such as Map-2, β-tubulin III, synaptophysin, neurogenin-1, Gap-43, and Drd-2. Furthermore, we observed an increase in generation of intracellular reactive oxygen species (ROS), activation of several kinases such as ERK and AKT, and downregulation of expression of dual-specificity phosphatases (DUSPs) in AgNPs-exposed SH-SY5Y cells. Our results suggest that AgNPs modulate the intracellular signaling pathways, leading to neuronal differentiation, and could be applied as promising nanomaterials for stem cell research and therapy.

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Silver Nanoparticles Promote Neuronal Growth

Cited by 18 publications

References 7 publications

Inorganic Nanomaterials in Tissue Engineering

Inorganic Nanomaterials in Tissue Engineering

Differential Cytotoxic Potential of Silver Nanoparticles in Human Ovarian Cancer Cells and Ovarian Cancer Stem Cells

Biologically synthesized silver nanoparticles induce neuronal differentiation of SH‐SY5Y cells via modulation of reactive oxygen species, phosphatases, and kinase signaling pathways

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