Silver Nanoparticles Synthesized Using Wild Mushroom Show Potential Antimicrobial Activities against Food Borne Pathogens

Abstract

The present study demonstrates an economical and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) using the wild mushroom Ganoderma sessiliforme. The synthesis of AgNPs was confirmed and the products characterized by UV-visible spectroscopy, dynamic light scattering spectroscopy and X-ray diffraction analysis. Furthermore, Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed to identify the viable biomolecules involved in the capping and active stabilization of AgNPs. Moreover, the average sizes and morphologies of AgNPs were analyzed by field emission scanning electron microscopy (FE-SEM). The potential impacts of AgNPs on food safety and control were evaluated by the antimicrobial activity of the synthesized AgNPs against common food-borne bacteria, namely, Escherichia coli, Bacillus subtilis, Streptococcus faecalis, Listeria innocua and Micrococcus luteus. The results of this study revealed that the synthesized AgNPs can be used to control the growth of food-borne pathogens and have potential application in the food packaging industry. Moreover, the AgNPs were evaluated for antioxidant activity (DPPH), for biocompatibility (L-929, normal fibroblast cells), and for cytotoxic effects on human breast adenosarcoma cells (MCF-7 & MDA-MB231) to highlight their potential for use in a variety of bio-applications.

Microwave-assisted green synthesis of silver nanoparticles using dried extracts of Chlorella vulgaris and antibacterial activity studies

“…Owing to the SPR of AgNPs, the bands outlined above are ordinary absorption bands of these NPs. Noteworthy is the fact that the absorption of AgNPs was also reported between 410 and 440 nm in other research studies [2,16]. Figure 3 reveals that λ max of the synthesized AgNPs was obtained at 431 nm with a concentration of 59.22 ppm, an indication of the successfully formed AgNPs, which was in the desirable range for AgNPs.…”

“…Being nature friendly [2], affordable [12], maintainable [13], and free from chemical pollution [14], biological agents have become excellent candidates for the production of NPs. Furthermore, the green synthesis of NPs using microorganisms has become the center of attention over the past few years due to remarkable properties, such as lower synthesis time [15], and it is a low-priced, safe, reliable [16], and single-step [14] procedure. Due to the capping feature of components in microorganisms, addition of extra stabilizers is not required [11].…”

Insect wing extract: A novel source for green synthesis of nanoparticles of antioxidant and antimicrobial potential

“…The XRD pattern of MMAgNPs showed the diffraction peaks at 2θ values of 32.6°, 46.57°, 67.8° and 77.04° which further confirmed crystalline nature of MMAgNPs (Figure 4b) and corresponded to standard JCPDS file No. 04-0783 [28]. Broadening in peaks occurred due to smaller particle size, which reflects the experimental conditions on nucleation and growth of crystal nuclei [29].…”

Nanosilver‐Enabled Food Storage Container Tradeoffs: Environmental Impacts Versus Food Savings Benefit, Informed by Literature

“…Antimicrobial packaging is currently used or is under investigation for use in industries in applications such as meat and milk storage (Quintavalla and Vicini 2002;Mauriello et al 2005). Antimicrobial agents used include acetic acid, benzoic acid, benzoic anhydride, sodium benzoate, lactic acid, lauric acid, propionic acid, sorbic acid, potassium sorbate, glucose oxidase, lysozyme, nisin, pediocin, silver (both bulk and nanoscale), lysozyme, zinc oxide (nanoscale), parabens, and single-walled carbon nanotubes (Quintavalla and Vicini 2002;Cooksey 2005;Mauriello et al 2005;Jin and Zhang 2008;Gemili et al 2009;Espitia et al 2012;Kuorwel et al 2015;Cheng et al 2018;Mohanta et al 2018;Pellerito et al 2018).…”