Silver nanowires-based signal amplification for CdSe quantum dots electrochemiluminescence immunoassay

Huang, Teng Yi; Meng, Qingmin; Jie, Guifen

doi:10.1016/j.bios.2014.11.011

Cited by 34 publications

(11 citation statements)

References 24 publications

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“…In immunosensing and immunoassay, various materials such as enzymes [16,17], quantum dots [28], oligonucleotides [29] and dyes [30], have been used as signal tags for signal enhancement. Utilizing the direct electrochemical signal of metallic nanoparticles including gold nanoparticles (AuNPs) [31,32] and silver NPs [33,34] as labels, several substitute strategies have also been developed for ultrasensitive detection for protein marker and DNA hybridization.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical non-enzymatic immunosensor for ultrasensitive detection of microcystin-LR using carbon nanofibers as the matrix

Zhang

Sun

Dong

et al. 2016

Sensors and Actuators B: Chemical

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a b s t r a c tThis work designed an ultrasensitive non-enzymatic immunosensor for microcystin-LR (MC-LR) detection using carbon nanofibers (CNFs) as immobilization matrix and gold nanoparticles (AuNPs) as an electrochemical label. The carboxylic group functionalized CNFs could well disperse in water for at least three months, and CNFs modified electrode showed high conductivity and enhanced electron transfer. The immunosensor was constructed by immobilizing MC-LR antigen on CNFs modified electrode. With a competitive immunoassay format, the coated MC-LR antigen competed for MC-LR antibody with added target MC-LR to form antibody-antigen immunocomplex. Subsequently, the immunocomplex reacted with AuNPs labeled secondary antibodies, and the bound AuNPs were finally electro-oxidized in 0.1 M HCl to produce AuCl 4 − for differential pulse voltammetric detection of MC-LR. Under optimal conditions, the immunosensor exhibited a wide linear response to MC-LR ranging from 0.0025 to 5 g L −1 with a detection limit of 1.68 ng L −1 at a signal to noise of 3. This method showed good accuracy, acceptable precision and reproducibility. The assay results of MC-LR in polluted water were in a good agreement with the reference values. The proposed ultrasensitive immunoassay showed the promising application in environmental monitoring and public health.

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Section: Introductionmentioning

confidence: 99%

An electrochemical non-enzymatic immunosensor for ultrasensitive detection of microcystin-LR using carbon nanofibers as the matrix

Zhang

Sun

Dong

et al. 2016

Sensors and Actuators B: Chemical

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“…Up to now, electrochemiluminescence (ECL) with the advantages of wide dynamic concentration response range, high sensitivity, spatial controllability and low cost, is a light emission that motivated by the high-energy electron transfer reaction (Richter, 2004). Therefore, the ECL technique has become a very powerful method and been extensively used in the biorelated species analysis such as protein Huang et al, 2015), DNA Lou et al, 2015), toxins (Zamolo et al, 2012), and bacteria (Yang et al, 2012;Ahmed et al, 2013). Especially, the nucleic acid amplification strategy-based ECL biosensors have been widely developed to achieve the sensitive detection of miRNAs (Ding et al, 2015).…”

mentioning

confidence: 99%

Target-catalyzed hairpin assembly and intramolecular/intermolecular co-reaction for signal amplified electrochemiluminescent detection of microRNA

Wang

Zhao

et al. 2016

Biosensors and Bioelectronics

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“…For example, CdSe QD ECL with amplification from a novel silver nanowire has been applied to immunoglobulin G (IgG) detection in serum samples [76]. IgG is an antibody that plays an important role in the immune system.…”

Section: Proteins Enzymes and Disease Diagnosticsmentioning

confidence: 99%

“…In the presence of DA, the CdTeQDs were captured by the electrode and produced ECL with O 2 coreactant. IgG Sandwich immunoassay with CdSe SWNTs and Ag nanowires Serum 1.0 pg/ml 1.0 pg/ml-0.5 ng/ml [76] Alkaline phosphatase…”

Section: Small Moleculesmentioning

confidence: 99%

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A Review of Electrogenerated Chemiluminescent Biosensors for Assays in Biological Matrices

2016

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Electrogenerated chemiluminescence (ECL) is the production of light via electron transfer reactions between electrochemically produced reagents. ECL-based biosensors use specific biological interactions to recognize an analyte and produce a luminescent signal. Biosensors fabricated with novel biorecognition species have increased the number of analytes detected. Some of these analytes include peptides, cells, enzymes and nucleic acids. ECL biosensors are selective, simple, sensitive and have low detection limits. Traditional methods use ruthenium complexes or luminol to generate ECL. Nanomaterials can be incorporated into ECL biosensors to improve efficiency, but also represent a new class of ECL emitters. This article reviews the application of ruthenium complex, luminol and nanomaterial-based ECL biosensors to making measurements in biological matrices over the past 4 years.

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Silver nanowires-based signal amplification for CdSe quantum dots electrochemiluminescence immunoassay

Cited by 34 publications

References 24 publications

An electrochemical non-enzymatic immunosensor for ultrasensitive detection of microcystin-LR using carbon nanofibers as the matrix

An electrochemical non-enzymatic immunosensor for ultrasensitive detection of microcystin-LR using carbon nanofibers as the matrix

Target-catalyzed hairpin assembly and intramolecular/intermolecular co-reaction for signal amplified electrochemiluminescent detection of microRNA

A Review of Electrogenerated Chemiluminescent Biosensors for Assays in Biological Matrices

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