Silver nitrate (AgNO3) and Pyrazinamide (PZA) as ethylene inhibitors ameliorate in vitro salt tolerance of tomato (Lycopersicon esculentum L) plant.

Zarei, Somayeh; Ehsanpour, Ali Akbar

doi:10.21203/rs.3.rs-2577831/v1

Cited by 3 publications

(2 citation statements)

References 38 publications

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“…Ethylene, a phytohormone, is essential for regulating various aspects of plant growth and development. Several studies have shown that the buildup of ethylene in culture media can lead to reduced growth and morphological changes in plants [22][23][24]. To counteract these effects, compounds such as AgNO3, a silver salt, have been utilized as anti-ethylene agents in vitro cultures [25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization

Kovács,

Portocarrero,

Honfi

et al. 2024

Plants

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The endangered plant species Adenophora liliifolia faces threats to its survival in the wild, necessitating the development of effective micropropagation techniques for potential reintroduction efforts. This study demonstrates that Adenophora liliifolia effectively reproduces on MS synthetic medium with diverse plant growth regulators (PGR) and natural extracts, facilitating swift micropropagation for potential future reintroduction endeavors. It highlights the substantial impact of PGR composition and natural extracts on the growth and development of A. liliifolia. The ideal growth medium for A. liliifolia was determined to be ½ MS with specific treatments. Additionally, incorporating silver nitrate (AgNO3) at 5 mg L−1 into the medium led to enhanced root formation and shoot length, albeit excessive concentrations adversely affected root development. Varying concentrations of NAA significantly affected different plant growth parameters, with the 0.1 mg L−1 treatment yielding comparable plant height to the control. Moreover, 50 mL L−1 of coconut water bolstered root formation, while 200 mL L−1 increased shoot formation during in vitro propagation. However, elevated doses of coconut water (CW) impeded root development but stimulated shoot growth. Experiments measuring chlorophyll a + b and carotenoid content indicated higher concentrations in the control group than differing levels of applied coconut water. Optimizing pH levels from 6.8–7 to 7.8–8.0 notably enhanced plant height and root formation, with significant carotenoid accumulation observed at pH 6.8–7. Soil samples from A. liliifolia’s natural habitat exhibited a pH of 6.65. Ultimately, the refined in vitro propagation protocol effectively propagated A. liliifolia, representing a pioneering effort and setting the stage for future restoration initiatives and conservation endeavors.

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Section: Discussionmentioning

confidence: 99%

Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization

Kovács,

Portocarrero,

Honfi

et al. 2024

Plants

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“…Inhibiting ethylene biosynthesis and signaling has emerged as a promising approach to alleviate the negative effects of salinity stress in plants. The ability to modulate ethylene responses by ethylene inhibitors, such as PZA and AgNO3, has been reported in some plant species (Zarei and Ehsanpour 2023). PZA as a competitive inhibitor of ACS and ACO enzymes responsible for the rate-limiting step in ethylene biosynthesis, while AgNO3 interferes with ethylene signaling by forming a nonfunctional complex with ethylene receptors.…”

Section: Introductionmentioning

confidence: 99%

Impact of in vitro treatment of Pyrazinamide (PZA), AgNO3 and salinity on the Expression of ACS3, ACO1, EIN2 and EIL1 genes in Tomato (Lycopersicon esculentumL.) plants

Zarei,

Ehsanpour,

Javadirad

2023

Preprint

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Salinity stress is a major environmental factor that negatively impacts plant growth and agricultural productivity. Ethylene, a crucial plant hormone, plays a vital role in regulating various physiological processes in response to environmental stresses, including salinity stress. In this study, we investigated the effect of two ethylene inhibitors, PZA and AgNO3, on the expression of key ethylene synthesis and signaling genes in tomato plants (Lycopersicon esculentum L.) under salinity stress in in vitro conditions. Tomato seedlings were subjected to salinity stress by supplementing the growth medium with NaCl, and different concentrations of PZA and AgNO3 were applied. The expression patterns of ACS3, ACO1, EIN2, and EIL1 genes, involved in ethylene biosynthesis and signaling, were analyzed using real-time quantitative PCR (qPCR). Under salinity stress, the expression of ACS3 and ACO1 genes was upregulated, indicating increased ethylene production. However, the application of PZA and AgNO3 treatments effectively suppressed ACS3 and ACO1 gene expression by 23% and 14%, respectively. Similarly, the ethylene signaling genes, EIN2 and EIL1, exhibited increased expression under salinity stress, but their expression was significantly reduced by 60–88% with PZA and AgNO3 treatments. The study shows that PZA and AgNO3 inhibitors effectively improve stress tolerance in tomato plants under salinity stress. These inhibitors offer insights into ethylene-mediated responses and potential strategies for developing stress-resilient crop varieties. Further research is needed to explore interactions between inhibitors and the ethylene signaling pathway and validate their practical application in agriculture.

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The effect of pyrazinamide and AgNO3 as ethylene inhibitors on some growth and biochemical parameters of in vitro potato (Solanum tuberosum L.) culture

Delavari,

Ehsanpour,

Moazzami Farida

2024

jct

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Silver nitrate (AgNO3) and Pyrazinamide (PZA) as ethylene inhibitors ameliorate in vitro salt tolerance of tomato (Lycopersicon esculentum L) plant.

Cited by 3 publications

References 38 publications

Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization

Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization

Impact of in vitro treatment of Pyrazinamide (PZA), AgNO3 and salinity on the Expression of ACS3, ACO1, EIN2 and EIL1 genes in Tomato (Lycopersicon esculentumL.) plants

The effect of pyrazinamide and AgNO3 as ethylene inhibitors on some growth and biochemical parameters of in vitro potato (Solanum tuberosum L.) culture

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