Silver nitrate modulates organogenesis in Brassica juncea (L.) through differential antioxidant defense and hormonal gene expression

Paladi, Radha Krishna; Archana, N.; Suprasanna, Penna

doi:10.1016/j.scienta.2017.08.038

Cited by 18 publications

(10 citation statements)

References 39 publications

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“…Shoot regeneration from leaf discs or hypocotyl segments of transgenic lines of mustard (Table 2), expressing antisense ACO, was greatly enhanced compared to wild-type as reported by Pua and Lee [99]. Shoot regeneration (%) in these transgenic lines was similar (about 90%, Table 1) to that reported when a chemical approach using silver nitrate and AVG was used [98,99]. In lemon, ACC and ETH treatments seems to decrease shoot regeneration in adult nodal segments, while silver thiosulfate treatments enhanced it [92].…”

Section: Organogenesissupporting

confidence: 71%

“…Explants of wild-type or transgenic lines treated with ETH showed a highly decrease in regeneration capacity and, at the higher ETH concentration (100 µM) a complete inhibition of regeneration was reported. A considerable increase of shoot regeneration capacity from hypocotyls of mustard in presence of 3 mg.L -1 silver nitrate was also detected [98]. Also in mustard, both ethylene inhibitors AVG and silver nitrate enhanced the shoot regeneration in leaf discs and petioles [99], whereas combined treatments with ethylene inhibitors and ETH decreased the regeneration capacity in leaf explants.…”

Section: Organogenesismentioning

confidence: 90%

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Modulation of Organogenesis and Somatic Embryogenesis by Ethylene: An Overview

Neves¹,

Correia²,

Cavaleiro³

et al. 2021

Preprint

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Ethylene is a plant hormone controlling physiological and developmental processes such fruit maturation, hairy root formation and leaf abscission. Its effect on regeneration systems, such as organogenesis and somatic embryogenesis (SE), has been studied and progresses in molecular biology techniques have contributed to unveil mechanisms behind its effects. This compound affects regeneration differently, depending on the species, genotype and explant. In some species, ethylene seems to revert recalcitrance in genotypes with low regeneration capacity. However, its effect is not addictive, since in genotypes with high regeneration capacity this ability decreases in the presence of ethylene precursors, suggesting that regeneration is modulated by ethylene. Several lines of evidence have shown that the role of ethylene on regeneration is markedly connected to biotic and abiotic stresses as well as to hormonal-crosstalk, in particular with key regeneration hormones and growth regulators of the auxin and cytokinin families. Transcriptional factors of the ethylene response factor (ERF) family are regulated by ethylene and strongly connected to SE induction. Thus, an evident connection between ethylene, stress responses and regeneration capacity is markedly established. In this review the effect of ethylene and the way it interacts with other players during organogenesis and somatic embryogenesis is discussed.

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Section: Organogenesissupporting

confidence: 71%

Section: Organogenesismentioning

confidence: 90%

Modulation of Organogenesis and Somatic Embryogenesis by Ethylene: An Overview

Neves¹,

Correia²,

Cavaleiro³

et al. 2021

Preprint

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“…Silver ions affect the number of green regenerants obtained via anther culture ( Jha et al, 2007 ); induce embryogenic callus formation ( Vain et al, 1989 ; Songstad et al, 1991 ); stimulate shoot induction, shoot formation ( Purnhauser et al, 1987 ), and in vitro rooting ( Wu et al, 2006 ; Jha et al, 2007 ; Würschum et al, 2015 ); inhibit callus necrosis ( Wu et al, 2006 ); and affect organogenesis ( Paladi et al, 2017 ). Silver ions can also compete with copper ions to bind to the mitochondrial complex IV ( Beyer, 1976 ; Costa et al, 2010 ) and to function as a cofactor of ethylene receptors for ethylene binding ( Light et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Copper Ions Induce DNA Sequence Variation in Zygotic Embryo Culture-Derived Barley Regenerants

2021

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In vitro tissue culture could be exploited to study cellular mechanisms that induce sequence variation. Altering the metal ion composition of tissue culture medium affects biochemical pathways involved in tissue culture-induced variation. Copper ions are involved in the mitochondrial respiratory chain and Yang cycle. Copper ions may participate in oxidative mutations, which may contribute to DNA sequence variation. Silver ions compete with copper ions to bind to the complex IV subunit of the respiratory chain, thus affecting the Yang cycle and DNA methylation. The mechanisms underlying somaclonal variation are unknown. In this study, we evaluated embryo-derived barley regenerants obtained from a single double-haploid plant via embryo culture under varying copper and silver ion concentrations and different durations of in vitro culture. Morphological variation among regenerants and the donor plant was not evaluated. Methylation-sensitive Amplified Fragment Length Polymorphism analysis of DNA samples showed DNA methylation pattern variation in CG and CHG (H = A, C, or T) sequence contexts. Furthermore, modification of in vitro culture conditions explained DNA sequence variation, demethylation, and de novo methylation in the CHG context, as indicated by analysis of variance. Linear regression indicated that DNA sequence variation was related to de novo DNA methylation in the CHG context. Mediation analysis showed the role of copper ions as a mediator of sequence variation in the CHG context. No other contexts showed a significant sequence variation in mediation analysis. Silver ions did not act as a mediator between any methylation contexts and sequence variation. Thus, incorporating copper ions in the induction medium should be treated with caution.

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“…It improves the efficiency of shoot induction and regeneration as well as in vitro rooting [ 28 , 29 , 30 ]. Silver ions prevent callus necrosis [ 29 ], modulate organogenesis [ 31 ], intensify shoot formation [ 32 ], and green plant regeneration [ 28 ]. Therefore, in vitro tissue culture may utilize copper and silver ions as ingredients added to the medium to improve the efficiency of androgenesis or somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

CG Demethylation Leads to Sequence Mutations in an Anther Culture of Barley Due to the Presence of Cu, Ag Ions in the Medium and Culture Time

Bednarek

Orłowska

2020

IJMS

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During plant tissue cultures the changes affecting regenerants have a broad range of genetic and epigenetic implications. These changes can be seen at the DNA methylation and sequence variation levels. In light of the latest studies, DNA methylation change plays an essential role in determining doubled haploid (DH) regenerants. The present study focuses on exploring the relationship between DNA methylation in CG and CHG contexts, and sequence variation, mediated by microelements (CuSO4 and AgNO3) supplemented during barley anther incubation on induction medium. To estimate such a relationship, a mediation analysis was used based on the results previously obtained through metAFLP method. Here, an interaction was observed between DNA demethylation in the context of CG and the time of culture. It was also noted that the reduction in DNA methylation was associated with a total decrease in the amount of Cu and Ag ions in the induction medium. Moreover, the total increase in Cu and Ag ions increased sequence variation. The importance of the time of tissue culture in the light of the observed changes resulted from the grouping of regenerants obtained after incubation on the induction medium for 28 days. The present study demonstrated that under a relatively short time of tissue culture (28 days), the multiplication of the Cu2+ and Ag+ ion concentrations (‘Cu*Ag’) acts as a mediator of demethylation in CG context. Change (increase) in the demethylation in CG sequence results in the decrease of ‘Cu*Ag’, and that change induces sequence variation equal to the value of the indirect effect. Thus, Cu and Ag ions mediate sequence variation. It seems that the observed changes at the level of methylation and DNA sequence may accompany the transition from direct to indirect embryogenesis

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Silver nitrate modulates organogenesis in Brassica juncea (L.) through differential antioxidant defense and hormonal gene expression

Cited by 18 publications

References 39 publications

Modulation of Organogenesis and Somatic Embryogenesis by Ethylene: An Overview

Modulation of Organogenesis and Somatic Embryogenesis by Ethylene: An Overview

Copper Ions Induce DNA Sequence Variation in Zygotic Embryo Culture-Derived Barley Regenerants

CG Demethylation Leads to Sequence Mutations in an Anther Culture of Barley Due to the Presence of Cu, Ag Ions in the Medium and Culture Time

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