Silver oxide nanoparticles alleviate indomethacin-induced gastric injury: a novel antiulcer agent

Abstract

Silver and silver oxides are gaining interest in medical applications for their prominent antibacterial and antimicrobial potentials. Recent studies suggest that nanosilver oxide has remarkable anti-inflammatory effects and enhances wound healing. Nevertheless, its effect on gastric ulcer has not yet been illustrated. Thus the current study aimed to explore the prospect protective effect of nanosilver oxide against indomethacin-induced gastric ulcer. A new approach has been followed to synthesize nanosilver oxide. X-ray diffraction, UV-Vis spectroscopy and transition electron microscope techniques have been successfully used to characterize the synthesized nanoparticles. Treatment of ulcerated rats with different doses of nanosilver oxide especially (175 and 350 ppm/p.o.) alleviated adverse effects of indomethacin-induced gastric injury as demonstrated by decreasing ulcer index and elevating % of ulcer inhibition. These positive effects excelled those exerted by the reference antiulcer drug omeprazole. Nanosilver oxide suppressed gastric inflammation by reducing myeloperoxidase, tumor necrosis alpha, interleukin 1beta and interferon gamma. Moreover, nanosilver oxide halted gastric oxidative stress via inhibiting lipid peroxidation and enhancing glutathione and paraoxonase-1. Regarding gastric apoptosis, nanosilver oxide down regulated the expression of caspase 9, tumor protein 53, and nuclear factor kappa B and allograft inflammatory factor-1 genes. These findings emphasize the antiulcerogenic potential of nanosilver oxide against indomethacin-induced gastric ulcers which are multi-factorial including anti-inflammatory, antioxidant and antiapoptotic effects.

Green Synthesis of Silver Nanoparticles in the Presence of Polysaccharide: Optimization and Characterization

“…Peaks at 2θ 38, 1, 44.9, and 77.5°can be attributed to the (111), (200), and (311) crystalline planes of the facecentered cubic crystalline structure of metallic silver. Such data are in agreement with data presented in works [20,21]. A radiograph of the dispersed phase after 5 minutes of treatment with a plasma discharge without stabilizer is presented.…”

“…Journal of Nanomaterials and 67.48 can be indexed to (211), (220), and (222) planes of face-center cubic silver, respectively. These peaks corroborate with the standard Ag 2 O (JCPDS 76-1393)[21,22]. The possibility of forming Ag 2 O structures is also reported.Figure 4(с) shows a typical XRD pattern of silver nanoparticles obtained in the aqueous solution: peaks at 2θ values of 38.1, 44.9, and 77.5 deg can be attributed to the (111),…”

“…It has been shown in work [21] that, when a NP is obtained under the action of a plasma discharge without a stabilizer, the pH of the initial solution of silver nitrate Journal of Nanomaterials decreases from 6.6 to 2.5. In Figure 9, the change in the pH of the solution in the presence of sodium alginate during the formation of silver nanoparticles for two concentrations (0.3 mmol/l and 3.0 mmol/l Ag + ) is shown.…”

Antioxidant capacity of Nitraria retusa leaf extracts against mitomycin C-induced genetic toxicity in male mice

“…Additionally, integrity was assured with ethidium bromide-stain analysis of 28S and 18S bands by formaldehyde-containing agarose gel electrophoresis. Aliquots were used immediately for reverse transcription (Linjawi, Khalil, Hassanane, & Ahmed, 2015;Salem, Wahba, Eisa, El-Shamarka, & Khalil, 2017).…”

Gene expression, DNA and kidney damage induced by pirimiphos-methyl in male mice: Chemical composition and ameliorative role of Origanum majorana leaves extract

“…The pellet of RNA was stored in DEPC treated water and digest the DNA residues of isolated RNA by treated with RNAse-free DNAse kit (Invitrogen, Germany) according to kits instruction. RNA aliquots were stored at -20°C or utilized immediately for reverse transcription [22].…”