Silver percutaneous absorption after exposure to silver nanoparticles: A comparison study of three human skin graft samples used for clinical applications

Bianco, Carlotta; Adami, Gianpiero; Crosera, Matteo; Larese, F; Casarin, Stefania; Castagnoli, Carlotta; Stella, Maurizio; Maina, Giovanni

doi:10.1016/j.burns.2014.02.003

Cited by 41 publications

(18 citation statements)

References 36 publications

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“…21 Each piece of skin was clamped between the donor and the receptor compartment; the exposed skin area was 1.77 cm 2 and the average skin thickness was 1 mm (percentage relative error of 1% and repeatability expressed as relative standard deviation [RSD] of 5%). Skin integrity was assessed using electrical conductibility before and after the experiment as previously described, cells with a resistance ,3.95±0.27 kΩ cm -2 were considered to be damaged and rejected as suggested by Bianco et al and Davies et al 22,23 Percutaneous penetration was investigated using static diffusion cells according to the Franz method. 24 Each donor chamber was filled with 1.5 mL of synthetic sweat (Milli-Q water, 0.5% NaCl, 0.1% lactic acid, and 0.1% urea, with ammonium hydroxide to reach a pH of 4.5) in which a sample of silver material (1.77 cm 2 ) was soaked.…”

Section: In Vitro Testmentioning

confidence: 99%

In vitro percutaneous penetration and characterization of silver from silver-containing textiles

Bianco

Kežić

Crosera

et al. 2015

IJN

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The objective of this study was to determine the in vitro percutaneous penetration of silver and characterize the silver species released from textiles in different layers of full thickness human skin. For this purpose, two different wound dressings and a garment soaked in artificial sweat were placed in the donor compartments of Franz cells for 24 hours. The concentration of silver in the donor phase and in the skin was determined by an electrothermal atomic absorption spectrometer (ET-AAS) and by inductively coupled plasma mass spectrometer (ICP-MS). The characterization of silver species in the textiles and in the skin layers was made by scanning electron microscopy with integrated energy dispersive X-ray spectroscopy (SEM-EDX). Additionally, the size distribution of silver nanoparticles in the textiles was performed by atomic force microscopy (AFM). On the surface of all investigated materials, silver nanoparticles of different size and morphology were found. Released silver concentrations in the soaking solutions (ie, exposure concentration) ranged from 0.7 to 4.7 µg/mL (0.6–4.0 µg/cm2), fitting the bactericidal range. Silver and silver chloride aggregates at sizes of up to 1 µm were identified both in the epidermis and dermis. The large size of these particles suggests that the aggregation occurred in the skin. The formation of these aggregates likely slowed down the systemic absorption of silver. Conversely, these aggregates may form a reservoir enabling prolonged release of silver ions, which might lead to local effects

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Section: In Vitro Testmentioning

confidence: 99%

In vitro percutaneous penetration and characterization of silver from silver-containing textiles

Bianco

Kežić

Crosera

et al. 2015

IJN

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“…Silver (Ag) NPs, on the other hand, are one of the most broad spectrum antibacterial agents. AgNPs demonstrate more effective antimicrobial properties than ionic Ag owing to their better permeation/retention effects and are effectively used for wound and burn dressings, surgical instruments, and skin grafts . Depending on their size, shape, dielectric environment, and mutual electromagnetic interactions among particles in close proximity, AgNPs can also demonstrate localized surface plasmon resonance (LSPR) effects, which enables their applications in photothermal and thermolytic laser therapies …”

Section: Introductionmentioning

confidence: 99%

Metal nanoparticle‐hydrogel nanocomposites for biomedical applications – An atmospheric pressure plasma synthesis approach

Nolan

Sun

Falzon

et al. 2018

Plasma Processes & Polymers

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The development of multifunctional nanocomposite materials is of great interest for various biomedical applications. A popular approach to produce tailored nanocomposites is to incorporate functional nanoparticles into hydrogels. Here, a benign atmospheric pressure microplasma synthesis approach has been deployed for the synthesis of metal and alloy NPs in-situ in a poly (vinyl alcohol) hydrogel.The formation of gold, silver, and gold-silver alloy NPs was confirmed via spectroscopic and microscopic characterization techniques. The properties of the hydrogel were not compromised during formation of the composites. Practical applications of the NP/PVA nanocomposites has been demonstrated by anti-bacterial testing. This establishes AMP processing as a viable one-step technique for the fabrication of NP/hydrogel composites, with potential multifunctionality for a range of biomedical applications.

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“…Skin samples were stored at −25 °C for a period up to, but not exceeding, four months. It has been demonstrated that this procedure does not damage skin barrier properties [25,26]. On the day of the experiment, skin samples were defrosted in a physiological solution at room temperature for a 30-min period and 2 × 2 cm 2 pieces were cut from each skin specimen and mounted separately on the diffusion cells.…”

Section: Methodsmentioning

confidence: 99%

Cerium Oxide Nanoparticles Absorption through Intact and Damaged Human Skin

et al. 2019

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Cerium oxide (CeO2) nanoparticles (NPs) are used in polishing products and absorbents, as promoters in wound healing, and as organopesticide decontaminants. While systemic bioaccumulation and organ toxicity has been described after inhalation, data on CeO2 NPs’ transdermal permeation are lacking. Our study was an in vitro investigation of the permeation of 17-nm CeO2 NPs dispersed in synthetic sweat (1 g L−1) using excised human skin on Franz cells. Experiments were performed using intact and needle-abraded skin, separately. The average amount of Ce into intact and damaged skin samples was 3.64 ± 0.15 and 7.07 ± 0.78 µg cm−2, respectively (mean ± SD, p = 0.04). Ce concentration in the receiving solution was 2.0 ± 0.4 and 3.3 ± 0.7 ng cm−2 after 24 h (p = 0.008). The Ce content was higher in dermal layers of damaged skin compared to intact skin (2.93 ± 0.71 µg cm−2 and 0.39 ± 0.16 µg cm−2, respectively; p = 0.004). Our data showed a very low dermal absorption and transdermal permeation of cerium, providing a first indication of Ce skin uptake due to contact with CeO2.

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Silver percutaneous absorption after exposure to silver nanoparticles: A comparison study of three human skin graft samples used for clinical applications

Cited by 41 publications

References 36 publications

In vitro percutaneous penetration and characterization of silver from silver-containing textiles

In vitro percutaneous penetration and characterization of silver from silver-containing textiles

Metal nanoparticle‐hydrogel nanocomposites for biomedical applications – An atmospheric pressure plasma synthesis approach

Cerium Oxide Nanoparticles Absorption through Intact and Damaged Human Skin

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